IPO8 and FBXL10: New Reference Genes for Gene Expression Studies in Human Adipose Tissue

• Published in: Obesity (Silver Spring, Md.) Vol. 18; no. 5; pp. 897 - 903
• Main Authors: Pozo, Carmen Hurtado, Calvo, Rosa M., Vesperinas‐García, Gregorio, Gómez‐Ambrosi, Javier, Frühbeck, Gema, Corripio‐Sánchez, Ramón, Rubio, Miguel A., Obregon, Maria‐Jesus
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.05.2010
• Subjects: Adipocytes; Adipocytes - cytology; Adipocytes - metabolism; Adipose Tissue - metabolism; Arrays; beta Karyopherins - genetics; beta Karyopherins - metabolism; Body fat; Cell Differentiation; Cells, Cultured; F-Box Proteins - genetics; F-Box Proteins - metabolism; Female; Gastrointestinal surgery; Gene expression; Hormones; Humans; Jumonji Domain-Containing Histone Demethylases - genetics; Jumonji Domain-Containing Histone Demethylases - metabolism; Male; Obesity; Oligonucleotide Array Sequence Analysis; Oxidoreductases, N-Demethylating; Proteins; Reverse Transcriptase Polymerase Chain Reaction; Statistical methods; Thyroid gland; Triglycerides; Madrid Spain; Spain

Housekeeping genes frequently used in gene expression studies are highly regulated in human adipose tissue. To ensure a correct interpretation of results, it is critical to select appropriate reference genes. Subcutaneous (SC) and omental (OM) adipose tissue expression was analyzed from lean and obe...