Nuclear accumulation of HMG1 protein is correlated to DNA synthesis

The subcellular localization of HMG1 protein was studied by immunoelectron microscopy during growth of CV1 cells in culture and in confluent CV1 cells subsequently lytically infected with SV40. HMG1 was always detected in the cytoplasm of both non-infected and infected cells. On the other hand, this...

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Bibliographic Details
Published inBiology of the cell Vol. 58; no. 3; p. 185
Main Authors Bonne-Andrea, C, Harper, F, Puvion, E, Delpech, M, De Recondo, A M
Format Journal Article
LanguageEnglish
Published England 1986
Subjects
Animals
Cell Division
Cell Line
Cell Nucleus - metabolism
Cell Nucleus - ultrastructure
Cell Transformation, Viral
DNA - biosynthesis
DNA Replication
High Mobility Group Proteins - metabolism
Immunoenzyme Techniques
Microscopy, Electron
Simian virus 40 - genetics
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Summary:The subcellular localization of HMG1 protein was studied by immunoelectron microscopy during growth of CV1 cells in culture and in confluent CV1 cells subsequently lytically infected with SV40. HMG1 was always detected in the cytoplasm of both non-infected and infected cells. On the other hand, this protein displayed a nuclear localization only in those cells active in cellular and/or viral DNA replication, that is, in actively dividing non-infected cells and in confluent cells following SV40 infection. The combination of electron microscope immunocytochemistry and autoradiography revealed that during SV40 lytic infection, HMG1 accumulates at sites of active viral DNA replication. Since HMG1 is a single-stranded DNA binding protein and acts in vitro as a physiological nucleosome assembly factor, we suggest that its presence in the nucleus is related to its requirement in the DNA replication process.
ISSN:0248-4900
1768-322X
DOI:10.1111/j.1768-322X.1986.tb00505.x