Experimental scrapie in the mouse: electrophoretic and sedimentation properties of the partially purified agent

• Published in: Journal of neurochemistry Vol. 35; no. 3; p. 574
• Main Authors: Prusiner, S B, Garfin, D E, Cochran, S P, McKinley, M P, Groth, D F, Hadlow, W J, Race, R E, Eklund, C M
• Format: Journal Article
• Language: English
• Published: England 01.09.1980
• Subjects: Animals; Centrifugation, Density Gradient; Detergents - pharmacology; DNA, Viral - analysis; Electrophoresis, Polyacrylamide Gel; Female; Hot Temperature; Mice; Prions - analysis; Prions - drug effects; RNA, Viral - analysis; Viral Proteins - analysis

Some biochemical and biophysical properties of the scrapie agent in a partially purified fraction P5 from murine spleen are described in this communication. The agent was stable in the nonionic detergents Triton X-100 and Nonidet P40 and stable in the nondenaturing, anionic detergents sodium cholate...