Evaluation and mechanism study of Pien Tze Huang against EV-A71 infection

Hand, foot, and mouth disease (HFMD) caused by enterovirus A71 (EV-A71) infection, currently lacks specific preventive and therapeutic interventions. Here, we demonstrated that Pien Tze Huang (PZH) could dose-dependently inhibit EV-A71 replication at the cellular level, resulting in significant redu...

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Published inFrontiers in pharmacology Vol. 14; p. 1251731
Main Authors Wang, Huiqiang, Chen, Fenbei, Wang, Shicong, Li, Yuhuan, Liu, Ting, Li, Yinghong, Deng, Hongbin, Dong, Jingwen, Pang, Jing, Song, Danqing, Zhang, Dousheng, Yu, Juan, Wang, Yanxiang
Format Journal Article
LanguageEnglish
Published Frontiers Media S.A 26.10.2023
Subjects
cytokine
enterovirus A71
host
multicomponent drug
Pharmacology
Pien Tze Huang
proteomics
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Abstract Hand, foot, and mouth disease (HFMD) caused by enterovirus A71 (EV-A71) infection, currently lacks specific preventive and therapeutic interventions. Here, we demonstrated that Pien Tze Huang (PZH) could dose-dependently inhibit EV-A71 replication at the cellular level, resulting in significant reductions in EV-A71 virus protein 1 (VP1) expression and viral yields in Vero and human rhabdomyosarcoma cells. More importantly, we confirmed that PZH could protect mice from EV-A71 infection for the first time, with Ribavirin serving as a positive control. PZH treatment reduced EV-A71 VP1 protein expression, viral yields in infected muscles, and improved muscle pathology. Additionally, we conducted a preliminary mechanism study using quantitative proteomics. The results suggested that the suppression of the PI3K/AKT/mTOR and NF-κB signaling pathways may contribute to the anti-EV-A71 activity of PZH. These findings provide strong evidence supporting the potential therapeutic application of PZH for EV-A71 infection management.
AbstractList Hand, foot, and mouth disease (HFMD) caused by enterovirus A71 (EV-A71) infection, currently lacks specific preventive and therapeutic interventions. Here, we demonstrated that Pien Tze Huang (PZH) could dose-dependently inhibit EV-A71 replication at the cellular level, resulting in significant reductions in EV-A71 virus protein 1 (VP1) expression and viral yields in Vero and human rhabdomyosarcoma cells. More importantly, we confirmed that PZH could protect mice from EV-A71 infection for the first time, with Ribavirin serving as a positive control. PZH treatment reduced EV-A71 VP1 protein expression, viral yields in infected muscles, and improved muscle pathology. Additionally, we conducted a preliminary mechanism study using quantitative proteomics. The results suggested that the suppression of the PI3K/AKT/mTOR and NF-κB signaling pathways may contribute to the anti-EV-A71 activity of PZH. These findings provide strong evidence supporting the potential therapeutic application of PZH for EV-A71 infection management.
Author Chen, Fenbei
Wang, Shicong
Zhang, Dousheng
Pang, Jing
Wang, Yanxiang
Wang, Huiqiang
Song, Danqing
Yu, Juan
Dong, Jingwen
Li, Yinghong
Li, Yuhuan
Liu, Ting
Deng, Hongbin
AuthorAffiliation 1 Beijing Key Laboratory of Antimicrobial Agents , Institute of Medicinal Biotechnology , Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , China
2 Fujian Pien Tze Huang Enterprise Key Laboratory of Natural Medicine Research and Development , Zhangzhou , China
3 Institute for Drug Control , National Institute for Food and Drug Control , Beijing , China
AuthorAffiliation_xml – name: 3 Institute for Drug Control , National Institute for Food and Drug Control , Beijing , China
– name: 1 Beijing Key Laboratory of Antimicrobial Agents , Institute of Medicinal Biotechnology , Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , China
– name: 2 Fujian Pien Tze Huang Enterprise Key Laboratory of Natural Medicine Research and Development , Zhangzhou , China
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Edited by: Karunakaran Kalesh, Teesside University, United Kingdom
Reviewed by: Wei Ye, Air Force Medical University, China
Durbadal Ojha, National Institute of Allergy and Infectious Diseases (NIH), United States
These authors have contributed equally to this work and share first authorship
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  doi: 10.1186/s12929-019-0560-7
  contributor:
    fullname: Lin
– volume: 2016
  start-page: 5697571
  year: 2016
  ident: B16
  article-title: Efficacy of scutellaria baicalensis for the treatment of hand, foot, and mouth disease associated with encephalitis in patients infected with EV71: a multicenter, retrospective analysis
  publication-title: Biomed. Res. Int.
  doi: 10.1155/2016/5697571
  contributor:
    fullname: Lin
– volume: 244
  start-page: 111856
  year: 2019
  ident: B37
  article-title: Pien-Tze-Huang ameliorates hepatic fibrosis via suppressing NF-κB pathway and promoting HSC apoptosis
  publication-title: J. Ethnopharmacol.
  doi: 10.1016/j.jep.2019.111856
  contributor:
    fullname: Zheng
SSID ssj0000399364
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Snippet Hand, foot, and mouth disease (HFMD) caused by enterovirus A71 (EV-A71) infection, currently lacks specific preventive and therapeutic interventions. Here, we...
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StartPage 1251731
SubjectTerms cytokine
enterovirus A71
host
multicomponent drug
Pharmacology
Pien Tze Huang
proteomics
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Title Evaluation and mechanism study of Pien Tze Huang against EV-A71 infection
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