Effect of luteinizing hormone on follicle stimulating hormone-activated paracrine signalling in rat ovary
'Pure' follicle stimulating hormone (FSH) and luteinizing hormone (LH) are expected shortly to become available for pharmaceutical use in the clinical setting. To test the contribution of LH to optimal ovarian responsiveness to FSH, 21-day-old hypophysectomized, immature, female rats recei...
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Published in | Human reproduction (Oxford) Vol. 10; no. 1; p. 33 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
01.01.1995
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Abstract | 'Pure' follicle stimulating hormone (FSH) and luteinizing hormone (LH) are expected shortly to become available for pharmaceutical use in the clinical setting. To test the contribution of LH to optimal ovarian responsiveness to FSH, 21-day-old hypophysectomized, immature, female rats received four s.c. injections of recombinant human LH (rhLH; total dose 1-10 IU) and/or rhFSH (total dose 30-72 IU) given at 12-hourly intervals. At 48 h after the first injection, ovaries were removed, weighed and used to isolate granulosa and thecal/interstitial cells for assessment of basal and gonadotrophin-responsive steroidogenesis in vitro, or homogenized to extract total RNA for Northern analysis of 17-hydroxylase/C17-20-lyase (cytochrome P-450c17 alpha) mRNA. Serum oestradiol and uterine weight were measured as indices of ovarian oestrogen production; androstenedione was measured to reflect ovarian androgen production. Consistent with the two-cell, two-gonadotrophin model of oestrogen synthesis, increased ovarian oestrogen secretion only occurred if both rhFSH and rhLH were given simultaneously. Treatment with rhFSH alone stimulated ovarian weight gain and granulosa cell aromatase activity without oestrogen secretion, whereas rhLH alone stimulated thecal androgen synthesis and androgen secretion. When the total rhLH dose was fixed at 1 IU, giving rise to an unmeasurably low serum concentration of rhLH, additional treatment with rhFSH (30-72 IU) dose-dependently stimulated serum androgen concentrations as well as oestrogen concentrations. The approximately 2.0 kb-sized P-450c17 alpha mRNA transcript was undetectable in the ovaries of untreated control animals but was abundant in the ovaries of positive controls treated with 15 IU of pregnant mare serum gonadotrophin. |
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AbstractList | 'Pure' follicle stimulating hormone (FSH) and luteinizing hormone (LH) are expected shortly to become available for pharmaceutical use in the clinical setting. To test the contribution of LH to optimal ovarian responsiveness to FSH, 21-day-old hypophysectomized, immature, female rats received four s.c. injections of recombinant human LH (rhLH; total dose 1-10 IU) and/or rhFSH (total dose 30-72 IU) given at 12-hourly intervals. At 48 h after the first injection, ovaries were removed, weighed and used to isolate granulosa and thecal/interstitial cells for assessment of basal and gonadotrophin-responsive steroidogenesis in vitro, or homogenized to extract total RNA for Northern analysis of 17-hydroxylase/C17-20-lyase (cytochrome P-450c17 alpha) mRNA. Serum oestradiol and uterine weight were measured as indices of ovarian oestrogen production; androstenedione was measured to reflect ovarian androgen production. Consistent with the two-cell, two-gonadotrophin model of oestrogen synthesis, increased ovarian oestrogen secretion only occurred if both rhFSH and rhLH were given simultaneously. Treatment with rhFSH alone stimulated ovarian weight gain and granulosa cell aromatase activity without oestrogen secretion, whereas rhLH alone stimulated thecal androgen synthesis and androgen secretion. When the total rhLH dose was fixed at 1 IU, giving rise to an unmeasurably low serum concentration of rhLH, additional treatment with rhFSH (30-72 IU) dose-dependently stimulated serum androgen concentrations as well as oestrogen concentrations. The approximately 2.0 kb-sized P-450c17 alpha mRNA transcript was undetectable in the ovaries of untreated control animals but was abundant in the ovaries of positive controls treated with 15 IU of pregnant mare serum gonadotrophin. |
Author | Hillier, S G Howles, C M Smyth, C D Miró, F |
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CitedBy_id | crossref_primary_10_1016_S1472_6483_10_61991_8 crossref_primary_10_1155_2015_925691 crossref_primary_10_1093_humrep_del408 crossref_primary_10_1095_biolreprod63_4_1214 crossref_primary_10_1210_jc_2009_0262 crossref_primary_10_1016_S0303_7207_01_00469_5 crossref_primary_10_1002_mrd_10107 crossref_primary_10_1016_j_fertnstert_2013_04_016 crossref_primary_10_1016_S0950_3552_97_80036_3 crossref_primary_10_1038_jhg_2016_82 crossref_primary_10_1093_molehr_gap086 crossref_primary_10_1210_me_2011_1341 crossref_primary_10_1016_j_jep_2003_12_024 |
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SubjectTerms | Aldehyde-Lyases - genetics Androstenedione - biosynthesis Animals Aromatase - metabolism Cytochrome P-450 Enzyme System - genetics Drug Interactions Female Follicle Stimulating Hormone - administration & dosage Granulosa Cells - drug effects Granulosa Cells - enzymology Hormones - blood Humans Luteinizing Hormone - administration & dosage Organ Size - drug effects Ovary - anatomy & histology Ovary - drug effects Ovary - metabolism Rats Rats, Wistar Recombinant Proteins - administration & dosage RNA, Messenger - genetics RNA, Messenger - metabolism Steroid 17-alpha-Hydroxylase Theca Cells - drug effects Theca Cells - metabolism Uterus - anatomy & histology Uterus - drug effects |
Title | Effect of luteinizing hormone on follicle stimulating hormone-activated paracrine signalling in rat ovary |
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