Effects of prolonged fasting on AMPK signaling, gene expression, and mitochondrial respiratory chain content in skeletal muscle from lean and obese individuals

• Published in: American journal of physiology: endocrinology and metabolism Vol. 304; no. 9; pp. E1012 - E1021
• Main Authors: Wijngaarden, Marjolein A., van der Zon, Gerard C., van Dijk, Ko Willems, Pijl, Hanno, Guigas, Bruno
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.05.2013
• Subjects: Adult; AMP-Activated Protein Kinases - metabolism; AMP-Activated Protein Kinases - physiology; Antigens, CD - biosynthesis; Antigens, CD - genetics; Blotting, Western; Body Composition - physiology; Body weight; Body Weight - physiology; Calorimetry; Calorimetry, Indirect; Electron Transport - physiology; Fasting - metabolism; Female; Gene expression; Gene Expression - physiology; Glucose - metabolism; Heat-Shock Proteins - metabolism; Humans; Lipid Peroxidation - physiology; Male; Metabolism; Mitochondria, Muscle - metabolism; Muscle, Skeletal - metabolism; Musculoskeletal system; Obesity; Obesity - metabolism; Oxidation; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; Real-Time Polymerase Chain Reaction; Receptor, Insulin - biosynthesis; Receptor, Insulin - genetics; Signal transduction; Signal Transduction - physiology; Transcription Factors - metabolism; Weight control; AMPK; metabolic flexibility; fasting; skeletal muscle; mitochondria

Obesity in humans is often associated with metabolic inflexibility, but the underlying molecular mechanisms remain incompletely understood. The aim of the present study was to investigate how adaptation to prolonged fasting affects energy/nutrient-sensing pathways and metabolic gene expression in sk...