A Cyp1a2-Luciferase Transgenic CD-1 Mouse Model: Responses to Aryl Hydrocarbons Similar to the Humanized AhR Mice

• Published in: Toxicological sciences Vol. 82; no. 1; pp. 297 - 307
• Main Authors: Zhang, Weisheng, Moorthy, Bhagavatula, Chen, Min, Muthiah, Kathirvel, Coffee, Richard, Purchio, Anthony F., West, David B.
• Format: Journal Article
• Language: English
• Published: United States Oxford University Press 01.11.2004
• Subjects: Amino Acid Sequence; Animals; Animals, Outbred Strains; aryl hydrocarbon receptor; Cytochrome P-450 CYP1A2 - biosynthesis; Cytochrome P-450 CYP1A2 - genetics; cytochrome P450 1A2; drug metabolism; Female; Gene Expression Regulation - drug effects; gene regulation; Genes, Reporter - genetics; Humans; Hydrocarbons, Aromatic - toxicity; imaging; luciferase; Luciferases - biosynthesis; Luciferases - genetics; Male; Mice; Mice, Transgenic; Models, Animal; Molecular Sequence Data; Polymorphism, Genetic; Receptors, Aryl Hydrocarbon - metabolism; reporter; RNA, Messenger - metabolism; transgenic mice; xenobiotics

Here we describe a transgenic mouse model [Crl:CD-1(ICR)BR-Tg(Cyp1a2-luc)Xen] using luciferase as a reporter for Cyp1a2 gene regulation. An 8.4-kilobase mouse Cyp1a2 promoter driving the firefly luciferase gene was microinjected into single-cell-stage CD-1 mouse embryos. A transgenic mouse line was...