A Cyp1a2-Luciferase Transgenic CD-1 Mouse Model: Responses to Aryl Hydrocarbons Similar to the Humanized AhR Mice

• Published in: Toxicological sciences Vol. 82; no. 1; pp. 297 - 307
• Main Authors: Zhang, Weisheng, Moorthy, Bhagavatula, Chen, Min, Muthiah, Kathirvel, Coffee, Richard, Purchio, Anthony F., West, David B.
• Format: Journal Article
• Language: English
• Published: United States Oxford University Press 01.11.2004
• Subjects: Amino Acid Sequence; Animals; Animals, Outbred Strains; aryl hydrocarbon receptor; Cytochrome P-450 CYP1A2 - biosynthesis; Cytochrome P-450 CYP1A2 - genetics; cytochrome P450 1A2; drug metabolism; Female; Gene Expression Regulation - drug effects; gene regulation; Genes, Reporter - genetics; Humans; Hydrocarbons, Aromatic - toxicity; imaging; luciferase; Luciferases - biosynthesis; Luciferases - genetics; Male; Mice; Mice, Transgenic; Models, Animal; Molecular Sequence Data; Polymorphism, Genetic; Receptors, Aryl Hydrocarbon - metabolism; reporter; RNA, Messenger - metabolism; transgenic mice; xenobiotics
• ISSN: 1096-6080; 1096-0929; 1096-0929
• DOI: 10.1093/toxsci/kfh260

Here we describe a transgenic mouse model [Crl:CD-1(ICR)BR-Tg(Cyp1a2-luc)Xen] using luciferase as a reporter for Cyp1a2 gene regulation. An 8.4-kilobase mouse Cyp1a2 promoter driving the firefly luciferase gene was microinjected into single-cell-stage CD-1 mouse embryos. A transgenic mouse line was selected based on basal and induced levels of the transgene in mouse liver by an in vivo bioluminescent imaging method. The basal levels of the luciferase reporter in liver were expressed much higher than other tissues, which correlated well with the endogenous Cyp1a2 mRNA tissue distribution. Male signals were about 23-fold higher than females in liver. However, the Cyp1a2 mRNA showed no gender difference. When mice were challenged with xenobiotics, the liver luciferase signal was induced to various degrees. At the doses we used, the relative effects were phenobarbital > 2,3,7,8-tetrachlorodibenzo-p-dioxin > 3-methylcholanthrene > benzo[a]pyrene and β-naphthoflavone. Induction of the Cyp1a2-luc reporter was generally consistent with the endogenous Cyp1a2 mRNA. However, phenobarbital induction was unexpectedly higher, while β-naphthoflavone induction of the reporter was much lower than that of the endogenous Cyp1a2 gene. Induction of the Cyp1a2-luc transgene by aryl hydrocarbons (Ah) in the CD-1 background was much less than that found in the Ah responsive C57BL/6 mice, while being similar to the nonresponsive DBA/2 strain. Sequence analysis of the CD-1 Ah receptor (AhR) cDNA clones demonstrated that consensus sequence was identical to some of the Ah-responsive strains such as BALB/C and CBA/J mice. The 104-kD AhR protein was not detectable in CD-1 mice, while the 97-kD AhR was detected in the C57BL/6 mice by Western blot using an AhR antibody. Low expression of the AhR in CD-1 mice could be in part responsible for low responsiveness to Ah compounds. The findings demonstrated the outbred CD-1 mouse is a low-responsive strain, and the Cyp1a2-luc transgenic CD-1 mice can be used for studying the regulation of the mouse Cyp1a2 gene in an Ah low-responsive strain in real time using the bioluminescent imaging approach.