Simultaneous detection of potato viruses, PLRV, PVA, PVX and PVY from dormant potato tubers by TaqMan ® real-time RT-PCR

• Published in: Journal of virological methods Vol. 142; no. 1; pp. 1 - 9
• Main Authors: Agindotan, Bright O., Shiel, Patrick J., Berger, Philip H.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01.06.2007; Amsterdam Elsevier; New York, NY
• Subjects: Biological and medical sciences; DNA Primers; Fundamental and applied biological sciences. Psychology; Microbiology; Multiple virus detection; Multiplex detection; Plant Diseases - virology; Plant Viruses - classification; Plant Viruses - genetics; Plant Viruses - isolation & purification; Potato tubers; Potexvirus - genetics; Potexvirus - isolation & purification; Potyvirus - genetics; Potyvirus - isolation & purification; Real-time RT-PCR; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA Viruses - classification; RNA Viruses - genetics; RNA Viruses - isolation & purification; RNA, Viral - analysis; RNA, Viral - isolation & purification; Sensitivity and Specificity; Solanum tuberosum - virology; Taq Polymerase; TaqMan; Techniques used in virology; Virology; Real-time RT-PCR; Multiple virus detection; Potato tubers; TaqMan; Multiplex detection; Plant pathogen; Microbiology; Dicotyledones; Angiospermae; Luteoviridae; Potyvirus; Solanaceae; Potato leaf roll virus; Reverse transcription polymerase chain reaction; Solanum tuberosum; Method; Real time; Polerovirus; Virology; Virus; Potato virus Y; Spermatophyta; Tuber plant; Detection; Potyviridae

The requirements of sprouting dormant potato tubers for biological or serological assays or RNA extraction for nucleic acid and PCR assays add to the cost of virus screening. Recently, cheaper, reliable and more rapid methods for the screening of potato tuber-seed pieces for viruses have been develo...