A rapid, sensitive and selective colorimetric method for detection of ascorbic acid
•Developed a new colorimetric method for ascorbic acid detection.•The colorimetric detection of ascorbic acid is rapid, sensitive and selective.•Photo-synthesized silver NCs are powerful for growth of silver nanoparticles. In this work, we report a colorimetric method for detection of ascorbic acid...
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| Published in | Sensors and actuators. B, Chemical Vol. 221; pp. 708 - 716 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier B.V
01.12.2015
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| Subjects | |
| Online Access | Get full text |
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| Abstract | •Developed a new colorimetric method for ascorbic acid detection.•The colorimetric detection of ascorbic acid is rapid, sensitive and selective.•Photo-synthesized silver NCs are powerful for growth of silver nanoparticles.
In this work, we report a colorimetric method for detection of ascorbic acid based on growth of silver nanoparticles by a simple and green photo-catalytic route. This route contains two steps, photo-synthesis of silver nanoclusters (NCs) with papain under UV irradiation, and catalytic growth of silver nanoparticles (NPs) in the presence of ascorbic acid. The produced silver NCs at the first step is excellent catalyzer that could quickly catalyze the growth of silver NPs by Ag+ and ascorbic acid in 1min at room temperature. And thus, in the second step, the color of the mixture changed from colorless to yellow and a strong absorption band near 420nm could be found in their absorption spectra owing to localized surface plasmon resonance (LSPR) of produced silver NPs. We found that the absorbance changes at 420nm have a good relationship with ascorbic acid concentration, and established a spectrophotometric method for the sensing of ascorbic acid in the range from 0.25 to 50.0μM, with a limit of detection (LOD) as low as 79.2nM. We also established a colorimetric assay of ascorbic acid by analyzing the yellow value (Y%) of the silver NPs photographs using cyan, magenta and yellow (CMY) color mode. The lowest detection concentration of ascorbic acid for colorimetric assay by the photographs could be estimated to ∼1μM. Moreover, the method for ascorbic acid detection also has high selectivity. Potential interferes, such as glucose, dopamine, uric acid and cysteine will not affect the detection of ascorbic acid. |
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| AbstractList | In this work, we report a colorimetric method for detection of ascorbic acid based on growth of silver nanoparticles by a simple and green photo-catalytic route. This route contains two steps, photo-synthesis of silver nanoclusters (NCs) with papain under UV irradiation, and catalytic growth of silver nanoparticles (NPs) in the presence of ascorbic acid. The produced silver NCs at the first step is excellent catalyzer that could quickly catalyze the growth of silver NPs by Ag+ and ascorbic acid in 1min at room temperature. And thus, in the second step, the color of the mixture changed from colorless to yellow and a strong absorption band near 420nm could be found in their absorption spectra owing to localized surface plasmon resonance (LSPR) of produced silver NPs. We found that the absorbance changes at 420nm have a good relationship with ascorbic acid concentration, and established a spectrophotometric method for the sensing of ascorbic acid in the range from 0.25 to 50.0 mu M, with a limit of detection (LOD) as low as 79.2nM. We also established a colorimetric assay of ascorbic acid by analyzing the yellow value (Y%) of the silver NPs photographs using cyan, magenta and yellow (CMY) color mode. The lowest detection concentration of ascorbic acid for colorimetric assay by the photographs could be estimated to 1 mu M. Moreover, the method for ascorbic acid detection also has high selectivity. Potential interferes, such as glucose, dopamine, uric acid and cysteine will not affect the detection of ascorbic acid. •Developed a new colorimetric method for ascorbic acid detection.•The colorimetric detection of ascorbic acid is rapid, sensitive and selective.•Photo-synthesized silver NCs are powerful for growth of silver nanoparticles. In this work, we report a colorimetric method for detection of ascorbic acid based on growth of silver nanoparticles by a simple and green photo-catalytic route. This route contains two steps, photo-synthesis of silver nanoclusters (NCs) with papain under UV irradiation, and catalytic growth of silver nanoparticles (NPs) in the presence of ascorbic acid. The produced silver NCs at the first step is excellent catalyzer that could quickly catalyze the growth of silver NPs by Ag+ and ascorbic acid in 1min at room temperature. And thus, in the second step, the color of the mixture changed from colorless to yellow and a strong absorption band near 420nm could be found in their absorption spectra owing to localized surface plasmon resonance (LSPR) of produced silver NPs. We found that the absorbance changes at 420nm have a good relationship with ascorbic acid concentration, and established a spectrophotometric method for the sensing of ascorbic acid in the range from 0.25 to 50.0μM, with a limit of detection (LOD) as low as 79.2nM. We also established a colorimetric assay of ascorbic acid by analyzing the yellow value (Y%) of the silver NPs photographs using cyan, magenta and yellow (CMY) color mode. The lowest detection concentration of ascorbic acid for colorimetric assay by the photographs could be estimated to ∼1μM. Moreover, the method for ascorbic acid detection also has high selectivity. Potential interferes, such as glucose, dopamine, uric acid and cysteine will not affect the detection of ascorbic acid. |
| Author | Zhang, Ling-Yan Ling, Jian Ding, Zhong-Tao Peng, Jun Zhang, Xiu-Qing Cao, Qiu-E |
| Author_xml | – sequence: 1 givenname: Jun surname: Peng fullname: Peng, Jun – sequence: 2 givenname: Jian surname: Ling fullname: Ling, Jian email: lingjian@ynu.edu.cn – sequence: 3 givenname: Xiu-Qing surname: Zhang fullname: Zhang, Xiu-Qing – sequence: 4 givenname: Ling-Yan surname: Zhang fullname: Zhang, Ling-Yan – sequence: 5 givenname: Qiu-E surname: Cao fullname: Cao, Qiu-E email: qecao@ynu.edu.cn – sequence: 6 givenname: Zhong-Tao surname: Ding fullname: Ding, Zhong-Tao |
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| Snippet | •Developed a new colorimetric method for ascorbic acid detection.•The colorimetric detection of ascorbic acid is rapid, sensitive and... In this work, we report a colorimetric method for detection of ascorbic acid based on growth of silver nanoparticles by a simple and green photo-catalytic... |
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| SubjectTerms | Absorption spectra Ascorbic acid Assaying Catalytic-growth Color Colorimetric detection Colorimetry Nanoclusters Nanoparticles Silver Silver nanoparticles |
| Title | A rapid, sensitive and selective colorimetric method for detection of ascorbic acid |
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