A novel intracellular signal amplification strategy for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection

• Published in: Chemical communications (Cambridge, England) Vol. 56; no. 48; pp. 6579 - 6582
• Main Authors: Chen, Shengyu, Zhao, Jingjin, Yang, Xing, Zhao, Shulin, Liu, Yi-Ming
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 16.06.2020
• Subjects: adenosine triphosphate; Adenosine Triphosphate - analysis; Adenosine Triphosphate - metabolism; Amplification; Aptamers, Nucleotide - chemistry; Aptamers, Nucleotide - metabolism; Cell Line, Tumor; chemical compounds; chemical reactions; Electrophoresis; Electrophoresis, Microchip - methods; Firefly Luciferin - chemistry; fluorescence; Humans; Laser induced fluorescence; Lasers; Liposomes - chemistry; microchip technology; Microscopy, Confocal; Reproducibility of Results; Semiconductors; Spectrometry, Fluorescence

An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection. By using the method proposed, intracellular ATP levels in single HeLa, HepG2 and HL-7702 cells were found to be in the ra...