A novel intracellular signal amplification strategy for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection

An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection. By using the method proposed, intracellular ATP levels in single HeLa, HepG2 and HL-7702 cells were found to be in the ra...

Full description

Saved in:
Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 56; no. 48; pp. 6579 - 6582
Main Authors Chen, Shengyu, Zhao, Jingjin, Yang, Xing, Zhao, Shulin, Liu, Yi-Ming
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 16.06.2020
Subjects
adenosine triphosphate
Adenosine Triphosphate - analysis
Adenosine Triphosphate - metabolism
Amplification
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - metabolism
Cell Line, Tumor
chemical compounds
chemical reactions
Electrophoresis
Electrophoresis, Microchip - methods
Firefly Luciferin - chemistry
fluorescence
Humans
Laser induced fluorescence
Lasers
Liposomes - chemistry
microchip technology
Microscopy, Confocal
Reproducibility of Results
Semiconductors
Spectrometry, Fluorescence
Online AccessGet full text

Cover

Abstract An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection. By using the method proposed, intracellular ATP levels in single HeLa, HepG2 and HL-7702 cells were found to be in the range of 30-150, 30-140, and 19-120 fmol per cell, respectively. An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis laser-induced fluorescence detection.
AbstractList An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection. By using the method proposed, intracellular ATP levels in single HeLa, HepG2 and HL-7702 cells were found to be in the range of 30–150, 30–140, and 19–120 fmol per cell, respectively.
An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection. By using the method proposed, intracellular ATP levels in single HeLa, HepG2 and HL-7702 cells were found to be in the range of 30-150, 30-140, and 19-120 fmol per cell, respectively.An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection. By using the method proposed, intracellular ATP levels in single HeLa, HepG2 and HL-7702 cells were found to be in the range of 30-150, 30-140, and 19-120 fmol per cell, respectively.
An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection. By using the method proposed, intracellular ATP levels in single HeLa, HepG2 and HL-7702 cells were found to be in the range of 30-150, 30-140, and 19-120 fmol per cell, respectively. An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis laser-induced fluorescence detection.
Author Zhao, Jingjin
Zhao, Shulin
Chen, Shengyu
Yang, Xing
Liu, Yi-Ming
AuthorAffiliation Department of Chemistry and Biochemistry
State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources
Jackson State University
Guangxi Normal University
AuthorAffiliation_xml – name: State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources
– name: Jackson State University
– name: Guangxi Normal University
– name: Department of Chemistry and Biochemistry
Author_xml – sequence: 1
  givenname: Shengyu
  surname: Chen
  fullname: Chen, Shengyu
– sequence: 2
  givenname: Jingjin
  surname: Zhao
  fullname: Zhao, Jingjin
– sequence: 3
  givenname: Xing
  surname: Yang
  fullname: Yang, Xing
– sequence: 4
  givenname: Shulin
  surname: Zhao
  fullname: Zhao, Shulin
– sequence: 5
  givenname: Yi-Ming
  surname: Liu
  fullname: Liu, Yi-Ming
BackLink https://www.ncbi.nlm.nih.gov/pubmed/32400773$$D View this record in MEDLINE/PubMed
BookMark eNqFkk1rFTEUhoNU7Idu3CuRbkQYzWdnZnm59QsKuqjgbsgkZ-5NySTTJKPcf-TPNNPbWiii2STkPO8L57znGB344AGh55S8pYS37wzRmjBSM_UIHVF-Jiopmu8Hy1u2Vc2FPETHKV2RcqhsnqBDzgQhdc2P0K8V9uEHOGx9jkqDc7NTESe78cphNU7ODlarbIPHqRAZNjs8hIjzFvD1rHy-r4cBry6_Fqci9xsHeLFLuN_h0eoY9NZOGBzoHMO0DRGSTfinzVvsVIJYWW9mDQYPbl6KGrwGbCAXQXF_ih4PyiV4dnufoG8f3l-uP1UXXz5-Xq8uKs3bs1xB3ShGh56alvVK0EYIqgzXre6VZALKH_CaSmI4HVrFeC17ZgjwRsuWgeIn6PXed4rheoaUu9GmpRHlIcypY20ja8apIP9HBWGCc14v6OkD9CrMsYx4oUpMRDC5UC9vqbkfwXRTtKOKu-4urgKQPVDGmVKEodM23wy_RGNdR0m3bER3Ttbrm41YFcmbB5I717_Cr_ZwTPoPd79e3WSGwrz4F8N_A4v6zao
CitedBy_id crossref_primary_10_1016_j_bios_2022_114053
crossref_primary_10_1016_j_microc_2024_110742
crossref_primary_10_1039_D1CC00455G
crossref_primary_10_1039_D1CC04719A
crossref_primary_10_1002_anse_202200007
crossref_primary_10_1016_j_talanta_2020_121686
crossref_primary_10_1016_j_snb_2022_131400
crossref_primary_10_1021_acs_analchem_1c04244
crossref_primary_10_2116_analsci_20SAR12
Cites_doi 10.1038/nn1472
10.1021/acs.analchem.7b02763
10.1021/acs.analchem.6b04020
10.1038/nn.3980
10.1038/nature06189
10.1039/C7LC01333G
10.1016/j.bios.2015.12.015
10.1016/j.bios.2014.07.064
10.1038/323448a0
10.1126/science.1118435
10.1038/35185
10.1021/acs.analchem.6b05045
10.1039/C7AN02082A
10.1039/C6CC00110F
10.1039/C6CC08911A
10.1016/j.cell.2014.02.010
10.1038/sj.cdd.4400262
10.1002/ange.201601969
10.1021/ac901759g
10.1038/nature03690
10.1002/anie.201710273
ContentType Journal Article
Copyright Copyright Royal Society of Chemistry 2020
Copyright_xml – notice: Copyright Royal Society of Chemistry 2020
DBID AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
7SR
7U5
8BQ
8FD
JG9
L7M
7X8
7S9
L.6
DOI 10.1039/d0cc02072a
DatabaseName CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
Engineered Materials Abstracts
Solid State and Superconductivity Abstracts
METADEX
Technology Research Database
Materials Research Database
Advanced Technologies Database with Aerospace
MEDLINE - Academic
AGRICOLA
AGRICOLA - Academic
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
Materials Research Database
Engineered Materials Abstracts
Solid State and Superconductivity Abstracts
Technology Research Database
Advanced Technologies Database with Aerospace
METADEX
MEDLINE - Academic
AGRICOLA
AGRICOLA - Academic
DatabaseTitleList CrossRef
MEDLINE - Academic
Materials Research Database
MEDLINE
AGRICOLA
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Chemistry
EISSN 1364-548X
EndPage 6582
ExternalDocumentID 32400773
10_1039_D0CC02072A
d0cc02072a
Genre Journal Article
GroupedDBID -
0-7
0R
1TJ
29B
4.4
53G
5GY
70
705
70J
7~J
AAEMU
AAGNR
AAIWI
AANOJ
ABDVN
ABFLS
ABGFH
ABPTK
ABRYZ
ACGFS
ACIWK
ACLDK
ACNCT
ADMRA
ADSRN
AENEX
AFVBQ
AGKEF
AGSTE
AGSWI
ALMA_UNASSIGNED_HOLDINGS
ASKNT
AUDPV
AZFZN
BLAPV
BSQNT
C6K
CKLOX
CS3
DU5
DZ
EBS
ECGLT
EE0
EF-
F5P
GNO
H13
HZ
H~N
IDZ
IH2
J3I
JG
M4U
N9A
O9-
P2P
R7B
R7C
R7D
RCNCU
RIG
RPMJG
RRA
RRC
RSCEA
SJN
SKA
SKF
SKH
SLH
TN5
TWZ
UPT
VH6
VQA
WH7
X
X7L
---
-DZ
-~X
0R~
2WC
6J9
70~
AAHBH
AAJAE
AAMEH
AAWGC
AAXHV
AAXPP
AAYXX
ABASK
ABEMK
ABJNI
ABPDG
ABXOH
ACBEA
ACGFO
AEFDR
AENGV
AESAV
AETIL
AFLYV
AFOGI
AFRDS
AFRZK
AGEGJ
AGRSR
AHGCF
AKMSF
ALUYA
ANUXI
APEMP
CITATION
GGIMP
HZ~
R56
RAOCF
-JG
CGR
CUY
CVF
ECM
EIF
NPM
7SR
7U5
8BQ
8FD
JG9
L7M
7X8
7S9
L.6
ID FETCH-LOGICAL-c396t-e78a21fb1d92ba418441ad3c9cba524eba4e37150d31f9a2375b2d0e38c592ea3
ISSN 1359-7345
1364-548X
IngestDate Fri Jul 11 16:46:35 EDT 2025
Fri Jul 11 02:39:42 EDT 2025
Sun Jun 29 16:11:01 EDT 2025
Wed Feb 19 02:30:01 EST 2025
Thu Apr 24 23:07:25 EDT 2025
Tue Jul 01 04:09:07 EDT 2025
Sat Jan 08 04:02:19 EST 2022
Wed Nov 11 00:26:56 EST 2020
IsPeerReviewed true
IsScholarly true
Issue 48
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c396t-e78a21fb1d92ba418441ad3c9cba524eba4e37150d31f9a2375b2d0e38c592ea3
Notes 10.1039/d0cc02072a
Electronic supplementary information (ESI) available: Experimental section, supplementary figures and table. See DOI
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ORCID 0000-0002-2560-042X
0000-0003-1194-7665
PMID 32400773
PQID 2413504250
PQPubID 2047502
PageCount 4
ParticipantIDs proquest_miscellaneous_2402433370
pubmed_primary_32400773
crossref_citationtrail_10_1039_D0CC02072A
crossref_primary_10_1039_D0CC02072A
rsc_primary_d0cc02072a
proquest_journals_2413504250
proquest_miscellaneous_2985723140
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 20200616
PublicationDateYYYYMMDD 2020-06-16
PublicationDate_xml – month: 6
  year: 2020
  text: 20200616
  day: 16
PublicationDecade 2020
PublicationPlace England
PublicationPlace_xml – name: England
– name: Cambridge
PublicationTitle Chemical communications (Cambridge, England)
PublicationTitleAlternate Chem Commun (Camb)
PublicationYear 2020
Publisher Royal Society of Chemistry
Publisher_xml – name: Royal Society of Chemistry
References Wei (D0CC02072A-(cit14)/*[position()=1]) 2015; 63
Gourine (D0CC02072A-(cit7)/*[position()=1]) 2005; 436
Higgins (D0CC02072A-(cit2)/*[position()=1]) 1986; 323
Qin (D0CC02072A-(cit15)/*[position()=1]) 2017; 53
Johnson (D0CC02072A-(cit6)/*[position()=1]) 2015; 18
Mao (D0CC02072A-(cit8)/*[position()=1]) 2018; 57
Wei (D0CC02072A-(cit16)/*[position()=1]) 2018; 143
Tsujimoto (D0CC02072A-(cit21)/*[position()=1]) 1997; 4
Masse (D0CC02072A-(cit5)/*[position()=1]) 2007; 449
Fan (D0CC02072A-(cit10)/*[position()=1]) 2018; 18
Li (D0CC02072A-(cit12)/*[position()=1]) 2016; 52
Li (D0CC02072A-(cit11)/*[position()=1]) 2017; 89
Zhu (D0CC02072A-(cit13)/*[position()=1]) 2016; 79
Zhang (D0CC02072A-(cit19)/*[position()=1]) 2009; 81
Rakszewska (D0CC02072A-(cit9)/*[position()=1]) 2016; 128
Finger (D0CC02072A-(cit3)/*[position()=1]) 2005; 310
Zheng (D0CC02072A-(cit18)/*[position()=1]) 2017; 89
Junker (D0CC02072A-(cit20)/*[position()=1]) 2014; 157
Elston (D0CC02072A-(cit1)/*[position()=1]) 1998; 391
Tan (D0CC02072A-(cit17)/*[position()=1]) 2017; 89
Davalos (D0CC02072A-(cit4)/*[position()=1]) 2005; 8
References_xml – volume: 8
  start-page: 72
  year: 2005
  ident: D0CC02072A-(cit4)/*[position()=1]
  publication-title: Nat. Neurosci.
  doi: 10.1038/nn1472
– volume: 89
  start-page: 10941
  year: 2017
  ident: D0CC02072A-(cit18)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.7b02763
– volume: 89
  start-page: 1749
  year: 2017
  ident: D0CC02072A-(cit17)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.6b04020
– volume: 18
  start-page: 637
  year: 2015
  ident: D0CC02072A-(cit6)/*[position()=1]
  publication-title: Nat. Neurosci.
  doi: 10.1038/nn.3980
– volume: 449
  start-page: 1058
  year: 2007
  ident: D0CC02072A-(cit5)/*[position()=1]
  publication-title: Nature
  doi: 10.1038/nature06189
– volume: 18
  start-page: 1151
  year: 2018
  ident: D0CC02072A-(cit10)/*[position()=1]
  publication-title: Lab Chip
  doi: 10.1039/C7LC01333G
– volume: 79
  start-page: 205
  year: 2016
  ident: D0CC02072A-(cit13)/*[position()=1]
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2015.12.015
– volume: 63
  start-page: 311
  year: 2015
  ident: D0CC02072A-(cit14)/*[position()=1]
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2014.07.064
– volume: 323
  start-page: 448
  year: 1986
  ident: D0CC02072A-(cit2)/*[position()=1]
  publication-title: Nature
  doi: 10.1038/323448a0
– volume: 310
  start-page: 1495
  year: 2005
  ident: D0CC02072A-(cit3)/*[position()=1]
  publication-title: Science
  doi: 10.1126/science.1118435
– volume: 391
  start-page: 510
  year: 1998
  ident: D0CC02072A-(cit1)/*[position()=1]
  publication-title: Nature
  doi: 10.1038/35185
– volume: 89
  start-page: 4559
  year: 2017
  ident: D0CC02072A-(cit11)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.6b05045
– volume: 143
  start-page: 1468
  year: 2018
  ident: D0CC02072A-(cit16)/*[position()=1]
  publication-title: Analyst
  doi: 10.1039/C7AN02082A
– volume: 52
  start-page: 3673
  year: 2016
  ident: D0CC02072A-(cit12)/*[position()=1]
  publication-title: Chem. Commun.
  doi: 10.1039/C6CC00110F
– volume: 53
  start-page: 455
  year: 2017
  ident: D0CC02072A-(cit15)/*[position()=1]
  publication-title: Chem. Commun.
  doi: 10.1039/C6CC08911A
– volume: 157
  start-page: 8
  year: 2014
  ident: D0CC02072A-(cit20)/*[position()=1]
  publication-title: Cell
  doi: 10.1016/j.cell.2014.02.010
– volume: 4
  start-page: 429
  year: 1997
  ident: D0CC02072A-(cit21)/*[position()=1]
  publication-title: Cell Death Differ.
  doi: 10.1038/sj.cdd.4400262
– volume: 128
  start-page: 6810
  year: 2016
  ident: D0CC02072A-(cit9)/*[position()=1]
  publication-title: Angew. Chem.
  doi: 10.1002/ange.201601969
– volume: 81
  start-page: 8695
  year: 2009
  ident: D0CC02072A-(cit19)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/ac901759g
– volume: 436
  start-page: 108
  year: 2005
  ident: D0CC02072A-(cit7)/*[position()=1]
  publication-title: Nature
  doi: 10.1038/nature03690
– volume: 57
  start-page: 236
  year: 2018
  ident: D0CC02072A-(cit8)/*[position()=1]
  publication-title: Angew. Chem., Int. Ed.
  doi: 10.1002/anie.201710273
SSID ssj0000158
Score 2.38765
Snippet An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced...
SourceID proquest
pubmed
crossref
rsc
SourceType Aggregation Database
Index Database
Enrichment Source
Publisher
StartPage 6579
SubjectTerms adenosine triphosphate
Adenosine Triphosphate - analysis
Adenosine Triphosphate - metabolism
Amplification
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - metabolism
Cell Line, Tumor
chemical compounds
chemical reactions
Electrophoresis
Electrophoresis, Microchip - methods
Firefly Luciferin - chemistry
fluorescence
Humans
Laser induced fluorescence
Lasers
Liposomes - chemistry
microchip technology
Microscopy, Confocal
Reproducibility of Results
Semiconductors
Spectrometry, Fluorescence
Title A novel intracellular signal amplification strategy for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection
URI https://www.ncbi.nlm.nih.gov/pubmed/32400773
https://www.proquest.com/docview/2413504250
https://www.proquest.com/docview/2402433370
https://www.proquest.com/docview/2985723140
Volume 56
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1fb9MwELfK9gAviH-DjoGM4AVVGamdNMljVDoNNAbSWqk8RYnjsKGSljZBKp-Ib8bX4M523BSqCXhJK8ey3Nyv57vL7-4IeeEJlvdDN3VkzoUD2s9zQi6kE4D5HGSMi1w1g3l3PjideG-n_rTT-dliLdVVdiy-78wr-R-pwhjIFbNk_0GydlEYgO8gX7iChOH6VzKOe-X8m8SqGdUyxRC84pQiJQMrACBXvDAhud5KV6FdW1rh1zrVPCFrM8bjDxj9wODBTPZwuRUap1-QsieQ02Va5iwu5-CjX5m8OLC-5dIBz75GJkExq_GmUPoil5UiepVtC9hWKBDt1BQV-7X5Y0pB6-4irUjF0CSSXMDnp3XdCnnrt0ew789XFusfTSB82pzNrakXl8i_bwc8GPbFcXQ-pmFKYVil4bQqzorpjNdS49yPnIDrQpXH0owNPAf8s2lb9-ui5gbjuuSn0eQDXze5MVYBGGps54njcizYmrtCwFYD1jpXGy7B-fvkZHJ2loxH0_ENss_AnwGFvB-Pxm_OWpXOVCtZu_Gmki6PXm3W3rad_nCIwDxaNm1rlHk0vkNuG7-Gxhqkd0lHlvfITfvQ7pMfMVVgpVtgpRqsdAustAErBbBSACvdBiudFxTACitRDVaqwEqzNbVgpb-BlSJY6RZYaRus1IL1AZmcjMbDU8e0CXEEjwaVI4MwZf0i6-cRy1KvH4KFn4LmiUSW-syTMCZ5AI5PzvtFlDIe-BnLXclD4UdMpvyA7JXzUj4iNB2kWJEu9IMCPGew4zI0yGWBbCzwrVmXvGwEkAhTQx9bucwSxeXgUfLaHQ6VsOIueW7nLnTlmJ2zjho5JkazrBJ81-3jaep2yTN7G-SFTzMt5bzGOVhLlPPgujkR_BJw4DyY81BjxG4FC3G6QcC75ABAY4c3YOuSw903kkVeHF6_7cfk1uafe0T2qmUtn4DxXmVPDfB_AfwW-Sk
linkProvider Royal Society of Chemistry
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+novel+intracellular+signal+amplification+strategy+for+the+quantification+of+ATP+in+single+cells+by+microchip+electrophoresis+with+laser-induced+fluorescence+detection&rft.jtitle=Chemical+communications+%28Cambridge%2C+England%29&rft.au=Chen%2C+Shengyu&rft.au=Zhao%2C+Jingjin&rft.au=Yang%2C+Xing&rft.au=Zhao%2C+Shulin&rft.date=2020-06-16&rft.pub=Royal+Society+of+Chemistry&rft.issn=1359-7345&rft.eissn=1364-548X&rft.volume=56&rft.issue=48&rft.spage=6579&rft.epage=6582&rft_id=info:doi/10.1039%2Fd0cc02072a&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1359-7345&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1359-7345&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1359-7345&client=summon