A novel intracellular signal amplification strategy for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection

An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection. By using the method proposed, intracellular ATP levels in single HeLa, HepG2 and HL-7702 cells were found to be in the ra...

Full description

Saved in:
Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 56; no. 48; pp. 6579 - 6582
Main Authors Chen, Shengyu, Zhao, Jingjin, Yang, Xing, Zhao, Shulin, Liu, Yi-Ming
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 16.06.2020
Subjects
adenosine triphosphate
Adenosine Triphosphate - analysis
Adenosine Triphosphate - metabolism
Amplification
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - metabolism
Cell Line, Tumor
chemical compounds
chemical reactions
Electrophoresis
Electrophoresis, Microchip - methods
Firefly Luciferin - chemistry
fluorescence
Humans
Laser induced fluorescence
Lasers
Liposomes - chemistry
microchip technology
Microscopy, Confocal
Reproducibility of Results
Semiconductors
Spectrometry, Fluorescence
Online AccessGet full text

Cover

More Information
Summary:An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection. By using the method proposed, intracellular ATP levels in single HeLa, HepG2 and HL-7702 cells were found to be in the range of 30-150, 30-140, and 19-120 fmol per cell, respectively. An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis laser-induced fluorescence detection.
Bibliography:10.1039/d0cc02072a
Electronic supplementary information (ESI) available: Experimental section, supplementary figures and table. See DOI
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:1359-7345
1364-548X
1364-548X
DOI:10.1039/d0cc02072a