ApoB-INDEPENDENT ENZYME IMMUNOASSAY FOR LIPOPROTEIN(a) BY CAPTURE ON IMMOBILIZED LECTIN (JACALIN)

Enzyme immunoassay for lipoprotein(a) [Lp(a)] using antibodies to both apoB and apo(a) subunits (a-B assay) is shown to be affected by differential masking of apoB by apo(a) and the presence of LDL-Lp(a) adducts. An apoB-independent immunoassay by capturing Lp(a) through its O-glycans on microplate-...

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Published inJournal of immunoassay & immunochemistry Vol. 34; no. 2; pp. 166 - 179
Main Authors Sreekumar, Anuradha, Mandagini, Geetha, Subramanian, Sabarinath P., Sankunni, Appukuttan P.
Format Journal Article
LanguageEnglish
Published England Taylor & Francis Group 01.04.2013
Subjects
Adolescent
Adult
Antibodies - chemistry
Apolipoproteins A - chemistry
Apolipoproteins B - chemistry
enzyme-linked immunoassay
Humans
Immobilized Proteins - chemistry
Immunoenzyme Techniques
Immunoglobulin A - blood
jacalin
lipoprotein(a)
Lipoprotein(a) - blood
Peroxidase - chemistry
Plant Lectins - chemistry
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Summary:Enzyme immunoassay for lipoprotein(a) [Lp(a)] using antibodies to both apoB and apo(a) subunits (a-B assay) is shown to be affected by differential masking of apoB by apo(a) and the presence of LDL-Lp(a) adducts. An apoB-independent immunoassay by capturing Lp(a) through its O-glycans on microplate-coated lectin jacalin and quantitation using peroxidase-labeled anti-apo(a) (J-a assay) is described. J-a assay response is linear, more than twice as sensitive as a-B assay, and is suppressed only 18 ± 5% by non-Lp(a) O-glycan-containing proteins of serum. Wide variations in IgA did not significantly affect Lp(a) binding to jacalin (CV = 6.4%).
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ISSN:1532-1819
1532-4230
DOI:10.1080/15321819.2012.699493