Changes in plasma chemokine C-C motif ligand 2 levels during treatment with eicosapentaenoic acid predict outcome in patients undergoing surgery for colorectal cancer liver metastasis

The mechanism of the anti-colorectal cancer (CRC) activity of the omega-3 fatty acid eicosapentaenoic acid (EPA) is not understood. We tested the hypothesis that EPA reduces expression of chemokine C-C motif ligand 2 (CCL2), a pro-inflammatory chemokine with known roles in metastasis.We measured CCL...

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Published inOncotarget Vol. 7; no. 19; pp. 28139 - 28150
Main Authors Volpato, Milene, Perry, Sarah L, Marston, Gemma, Ingram, Nicola, Cockbain, Andrew J, Burghel, Heather, Mann, Jake, Lowes, David, Wilson, Erica, Droop, Alastair, Randerson-Moor, Juliette, Coletta, P Louise, Hull, Mark A
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LanguageEnglish
Published United States Impact Journals LLC 10.05.2016
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Abstract The mechanism of the anti-colorectal cancer (CRC) activity of the omega-3 fatty acid eicosapentaenoic acid (EPA) is not understood. We tested the hypothesis that EPA reduces expression of chemokine C-C motif ligand 2 (CCL2), a pro-inflammatory chemokine with known roles in metastasis.We measured CCL2 in clinical samples from a randomized trial of EPA in patients undergoing liver surgery for CRC liver metastasis (LM) and preclinical models. Genome-wide transcriptional profiling of tumors from EPA-treated patients was performed.EPA decreased CCL2 synthesis by CRC cells in a dose-dependent manner. CCL2 was localized to malignant epithelial cells in human CRCLM. EPA did not reduce CCL2 content in human or mouse tumors compare to control. However, EPA treatment was associated with decreased plasma CCL2 levels compared with controls (P=0.04). Reduction in plasma CCL2 following EPA treatment predicted improved disease-free survival (HR 0.32; P=0.003). Lack of 'CCL2 response' was associated with a specific CRCLM gene expression signature.In conclusion, reduction in plasma CCL2 in patients with CRCLM treated with EPA predicts better clinical outcome and a specific tumor gene expression profile. Further work is needed to validate CCL2 as a therapeutic response biomarker for omega-3 fatty acid treatment of CRC patients.
AbstractList The mechanism of the anti-colorectal cancer (CRC) activity of the omega-3 fatty acid eicosapentaenoic acid (EPA) is not understood. We tested the hypothesis that EPA reduces expression of chemokine C-C motif ligand 2 (CCL2), a pro-inflammatory chemokine with known roles in metastasis.We measured CCL2 in clinical samples from a randomized trial of EPA in patients undergoing liver surgery for CRC liver metastasis (LM) and preclinical models. Genome-wide transcriptional profiling of tumors from EPA-treated patients was performed.EPA decreased CCL2 synthesis by CRC cells in a dose-dependent manner. CCL2 was localized to malignant epithelial cells in human CRCLM. EPA did not reduce CCL2 content in human or mouse tumors compare to control. However, EPA treatment was associated with decreased plasma CCL2 levels compared with controls (P=0.04). Reduction in plasma CCL2 following EPA treatment predicted improved disease-free survival (HR 0.32; P=0.003). Lack of 'CCL2 response' was associated with a specific CRCLM gene expression signature.In conclusion, reduction in plasma CCL2 in patients with CRCLM treated with EPA predicts better clinical outcome and a specific tumor gene expression profile. Further work is needed to validate CCL2 as a therapeutic response biomarker for omega-3 fatty acid treatment of CRC patients.
The mechanism of the anti-colorectal cancer (CRC) activity of the omega-3 fatty acid eicosapentaenoic acid (EPA) is not understood. We tested the hypothesis that EPA reduces expression of chemokine C-C motif ligand 2 (CCL2), a pro-inflammatory chemokine with known roles in metastasis. We measured CCL2 in clinical samples from a randomized trial of EPA in patients undergoing liver surgery for CRC liver metastasis (LM) and preclinical models. Genome-wide transcriptional profiling of tumors from EPA-treated patients was performed. EPA decreased CCL2 synthesis by CRC cells in a dose-dependent manner. CCL2 was localized to malignant epithelial cells in human CRCLM. EPA did not reduce CCL2 content in human or mouse tumors compare to control. However, EPA treatment was associated with decreased plasma CCL2 levels compared with controls (P=0.04). Reduction in plasma CCL2 following EPA treatment predicted improved disease-free survival (HR 0.32; P=0.003). Lack of ‘CCL2 response’ was associated with a specific CRCLM gene expression signature. In conclusion, reduction in plasma CCL2 in patients with CRCLM treated with EPA predicts better clinical outcome and a specific tumor gene expression profile. Further work is needed to validate CCL2 as a therapeutic response biomarker for omega-3 fatty acid treatment of CRC patients.
Author Cockbain, Andrew J
Randerson-Moor, Juliette
Burghel, Heather
Coletta, P Louise
Volpato, Milene
Hull, Mark A
Perry, Sarah L
Ingram, Nicola
Lowes, David
Droop, Alastair
Mann, Jake
Wilson, Erica
Marston, Gemma
AuthorAffiliation 3 MRC Medical Bioinformatics Centre, University of Leeds, Leeds, LS2 9NL, UK
1 Leeds Institute of Biomedical & Clinical Sciences, Wellcome Trust Brenner Building, St James's University Hospital, Leeds LS9 7TF, United Kingdom
2 Leeds Institute of Cancer Studies and Pathology, Wellcome Trust Brenner Building, St James's University Hospital, Leeds LS9 7TF, United Kingdom
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Keywords colorectal cancer
eicosapentaenoic acid and biomarker
prognosis
molecular pharmacology
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Snippet The mechanism of the anti-colorectal cancer (CRC) activity of the omega-3 fatty acid eicosapentaenoic acid (EPA) is not understood. We tested the hypothesis...
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SubjectTerms Animals
Antineoplastic Agents - therapeutic use
Biomarkers, Tumor - blood
Chemokine CCL2 - blood
Colorectal Neoplasms - drug therapy
Colorectal Neoplasms - mortality
Colorectal Neoplasms - pathology
Disease-Free Survival
Eicosapentaenoic Acid - therapeutic use
Female
Humans
Kaplan-Meier Estimate
Liver Neoplasms - drug therapy
Liver Neoplasms - secondary
Liver Neoplasms - surgery
Male
Mice
Mice, Inbred C57BL
Research Paper
Treatment Outcome
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Title Changes in plasma chemokine C-C motif ligand 2 levels during treatment with eicosapentaenoic acid predict outcome in patients undergoing surgery for colorectal cancer liver metastasis
URI https://www.ncbi.nlm.nih.gov/pubmed/27058904
https://search.proquest.com/docview/1812878628
https://pubmed.ncbi.nlm.nih.gov/PMC5053715
Volume 7
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