Intra- and inter-laboratory reproducibility and accuracy of the LuSens assay: A reporter gene-cell line to detect keratinocyte activation by skin sensitizers

• Published in: Toxicology in vitro Vol. 32; pp. 278 - 286
• Main Authors: Ramirez, Tzutzuy, Stein, Nadine, Aumann, Alexandra, Remus, Tina, Edwards, Amber, Norman, Kimberly G., Ryan, Cindy, Bader, Jackie E., Fehr, Markus, Burleson, Florence, Foertsch, Leslie, Wang, Xiaohong, Gerberick, Frank, Beilstein, Paul, Hoffmann, Sebastian, Mehling, Annette, van Ravenzwaay, Bennard, Landsiedel, Robert
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.04.2016
• Subjects: Allergens - toxicity; Animal Testing Alternatives; Antioxidant Response Elements - genetics; Biological Assay; Cell Line; Dermatitis, Contact; Genes, Reporter; Humans; Keratinocytes - drug effects; Keratinocytes - metabolism; Laboratories; Luciferases - genetics; Luciferases - metabolism; NF-E2-Related Factor 2 - genetics; Reproducibility of Results; Sensitivity and Specificity

Several non-animal methods are now available to address the key events leading to skin sensitization as defined by the adverse outcome pathway. The KeratinoSens™ assay addresses the cellular event of keratinocyte activation and is a method accepted under OECD TG 442D. In this study, the results of a...