Intra- and inter-laboratory reproducibility and accuracy of the LuSens assay: A reporter gene-cell line to detect keratinocyte activation by skin sensitizers

• Published in: Toxicology in vitro Vol. 32; pp. 278 - 286
• Main Authors: Ramirez, Tzutzuy, Stein, Nadine, Aumann, Alexandra, Remus, Tina, Edwards, Amber, Norman, Kimberly G., Ryan, Cindy, Bader, Jackie E., Fehr, Markus, Burleson, Florence, Foertsch, Leslie, Wang, Xiaohong, Gerberick, Frank, Beilstein, Paul, Hoffmann, Sebastian, Mehling, Annette, van Ravenzwaay, Bennard, Landsiedel, Robert
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.04.2016
• Subjects: Allergens - toxicity; Animal Testing Alternatives; Antioxidant Response Elements - genetics; Biological Assay; Cell Line; Dermatitis, Contact; Genes, Reporter; Humans; Keratinocytes - drug effects; Keratinocytes - metabolism; Laboratories; Luciferases - genetics; Luciferases - metabolism; NF-E2-Related Factor 2 - genetics; Reproducibility of Results; Sensitivity and Specificity
• ISSN: 0887-2333; 1879-3177
• DOI: 10.1016/j.tiv.2016.01.004

Several non-animal methods are now available to address the key events leading to skin sensitization as defined by the adverse outcome pathway. The KeratinoSens™ assay addresses the cellular event of keratinocyte activation and is a method accepted under OECD TG 442D. In this study, the results of an inter-laboratory evaluation of the “me-too” LuSens assay, a bioassay that uses a human keratinocyte cell line harboring a reporter gene construct composed of the rat antioxidant response element (ARE) of the NADPH:quinone oxidoreductase 1 gene and the luciferase gene, are described. Earlier in-house validation with 74 substances showed an accuracy of 82% in comparison to human data. When used in a battery of non-animal methods, even higher predictivity is achieved. To meet European validation criteria, a multicenter study was conducted in 5 laboratories. The study was divided into two phases, to assess 1) transferability of the method, and 2) reproducibility and accuracy. Phase I was performed by testing 8 non-coded test substances; the results showed a good transferability to naïve laboratories even without on-site training. Phase II was performed with 20 coded test substances (performance standards recommended by OECD, 2015). In this phase, the intra- and inter-laboratory reproducibility as well as accuracy of the method was evaluated. The data demonstrate a remarkable reproducibility of 100% and an accuracy of over 80% in identifying skin sensitizers, indicating a good concordance with in vivo data. These results demonstrate good transferability, reliability and accuracy of the method thereby achieving the standards necessary for use in a regulatory setting to detect skin sensitizers. •Keratinocyte activation is a key event of the skin sensitization AOP.•ARE–Nrf2-luciferase methods are accepted under OECD 442D to address this key event.•LuSens is a human keratinocyte line with an ARE–Nrf2-luciferase reporter.•Results of an inter-laboratory evaluation conducted in five laboratories•Reproducibility of 100% and accuracy in identifying skin sensitizers over 80%