The N-terminal Proline Hinge Motif Controls the Structure of Bovine Herpesvirus 1-encoded Inhibitor of the Transporter Associated with Antigen Processing Required for its Immunomodulatory Function

• Published in: Journal of molecular biology Vol. 435; no. 5; p. 167964
• Main Authors: Graul, Małgorzata, Karska, Natalia, Wąchalska, Magda, Krupa, Paweł, Ślusarz, Magdalena J., Lubocki, Marcin, Bieńkowska-Szewczyk, Krystyna, Rodziewicz-Motowidło, Sylwia, Sieradzan, Adam K., Lipińska, Andrea D.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.03.2023
• Subjects: Amino Acid Motifs; Antigen Presentation; herpesvirus; Herpesvirus 1, Bovine - immunology; Membrane Transport Proteins - metabolism; MHC class I; molecular dynamics; Proline - chemistry; Proline - genetics; Protein Transport; TAP transporter; UL49.5; BoHV-1; TAP; FL; PLC; CTLs; MHC; NBD; HSV-1; UNRES; herpesvirus; MD; MJS; cryo-EM; aa; MOI; IP; MHC class I; RMSD; TMD; molecular dynamics; ER; TAP transporter; UL49.5; ERAD; PAA; PE; TM; SASA

[Display omitted] •UL49.5 of BoHV-1 inhibits TAP transporter by mechanisms undetermined on a molecular level.•Mutations in 52PPQ54 proline hinge resulted in impaired TAP binding and inhibition.•According to MD, PPQ membrane anchor is key for UL49.5 structure and dynamical behavior.•52PPQ54 is requir...