Use of radiographic images for rapid and non-destructive assessment of crambe seed quality

Technologies based on electromagnetic radiation, such as the X-ray technique, has contributed to the establishment of new and promising methodologies for evaluating seed quality. This study aimed to relate parameters based on semi-automated analysis of radiographs of crambe seeds to their physiologi...

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Published inJournal of seed science Vol. 43
Main Authors Ribeiro, Alessandra da Silva, Neri, Tássia Fernanda Santos, Medeiros, André Dantas de, Milagres, Carla do Carmo, Silva, Laércio Junio da
Format Journal Article
LanguageEnglish
Published ABRATES - Associação Brasileira de Tecnologia de Sementes 01.01.2021
Associação Brasileira de Tecnologia de Sementes
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Online AccessGet full text
ISSN2317-1537
2317-1545
2317-1545
DOI10.1590/2317-1545v43239136

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Abstract Technologies based on electromagnetic radiation, such as the X-ray technique, has contributed to the establishment of new and promising methodologies for evaluating seed quality. This study aimed to relate parameters based on semi-automated analysis of radiographs of crambe seeds to their physiological quality. Radiographic images of seeds from 10 seed lots of cultivar FMS Brilhante were semi-automatically analyzed using ImageJ® software. Measurements of morphometric characteristics and tissue integrity were obtained for the seeds, as well as individually for the seed embryo. Following X-ray test, the seeds were subject to germination and seedling growth test. It was possible to visualize the internal structures of the seeds in the radiographs. There were differences in the physical parameters obtained by the semi-automated analysis of the radiographs between the seed lots. Also, the lots differed regarding the physiological quality of the seeds. Morphometric characteristics and tissue integrity, especially for the seed embryo, showed high correlation with the seed physiological quality. Therefore, this work presents an efficient approach to rapid and non-destructively assess the quality of crambe seeds. Resumo: Tecnologias baseadas em radiação eletromagnética, como a técnica de raios X, contribuíram para estabelecer novas e promissoras metodologias para avaliar a qualidade de sementes. Nesse sentido, este estudo teve como objetivo relacionar parâmetros baseados na análise semiautomatizada de radiografias de sementes de crambe à sua qualidade fisiológica. Imagens radiográficas de sementes pertencentes a 10 lotes da cultivar FMS Brilhante foram analisadas de forma semiautomatizada por meio do software ImageJ®. Foram obtidas medidas de características morfométricas e integridade tecidual das sementes, bem como individualmente para o embrião das sementes. Após o teste de raio-X, as sementes foram submetidas ao teste de germinação e crescimento de plântulas. Foi possível visualizar as estruturas internas das sementes nas radiografias. Houve diferenças nos parâmetros físicos obtidos pela análise semiautomática das radiografias entre os lotes de sementes. Além disso, os lotes diferiram quanto à qualidade fisiológica das sementes. Características morfométricas e de integridade tecidual, especialmente para o embrião, apresentaram alta correlação com a qualidade fisiológica. Assim, este trabalho apresenta uma abordagem eficiente para avaliar de forma rápida e não destrutiva a qualidade de sementes de crambe.
AbstractList Technologies based on electromagnetic radiation, such as the X-ray technique, has contributed to the establishment of new and promising methodologies for evaluating seed quality. This study aimed to relate parameters based on semi-automated analysis of radiographs of crambe seeds to their physiological quality. Radiographic images of seeds from 10 seed lots of cultivar FMS Brilhante were semi-automatically analyzed using ImageJ® software. Measurements of morphometric characteristics and tissue integrity were obtained for the seeds, as well as individually for the seed embryo. Following X-ray test, the seeds were subject to germination and seedling growth test. It was possible to visualize the internal structures of the seeds in the radiographs. There were differences in the physical parameters obtained by the semi-automated analysis of the radiographs between the seed lots. Also, the lots differed regarding the physiological quality of the seeds. Morphometric characteristics and tissue integrity, especially for the seed embryo, showed high correlation with the seed physiological quality. Therefore, this work presents an efficient approach to rapid and non-destructively assess the quality of crambe seeds. Resumo: Tecnologias baseadas em radiação eletromagnética, como a técnica de raios X, contribuíram para estabelecer novas e promissoras metodologias para avaliar a qualidade de sementes. Nesse sentido, este estudo teve como objetivo relacionar parâmetros baseados na análise semiautomatizada de radiografias de sementes de crambe à sua qualidade fisiológica. Imagens radiográficas de sementes pertencentes a 10 lotes da cultivar FMS Brilhante foram analisadas de forma semiautomatizada por meio do software ImageJ®. Foram obtidas medidas de características morfométricas e integridade tecidual das sementes, bem como individualmente para o embrião das sementes. Após o teste de raio-X, as sementes foram submetidas ao teste de germinação e crescimento de plântulas. Foi possível visualizar as estruturas internas das sementes nas radiografias. Houve diferenças nos parâmetros físicos obtidos pela análise semiautomática das radiografias entre os lotes de sementes. Além disso, os lotes diferiram quanto à qualidade fisiológica das sementes. Características morfométricas e de integridade tecidual, especialmente para o embrião, apresentaram alta correlação com a qualidade fisiológica. Assim, este trabalho apresenta uma abordagem eficiente para avaliar de forma rápida e não destrutiva a qualidade de sementes de crambe.
Technologies based on electromagnetic radiation, such as the X-ray technique, has contributed to the establishment of new and promising methodologies for evaluating seed quality. This study aimed to relate parameters based on semi-automated analysis of radiographs of crambe seeds to their physiological quality. Radiographic images of seeds from 10 seed lots of cultivar FMS Brilhante were semi-automatically analyzed using ImageJ® software. Measurements of morphometric characteristics and tissue integrity were obtained for the seeds, as well as individually for the seed embryo. Following X-ray test, the seeds were subject to germination and seedling growth test. It was possible to visualize the internal structures of the seeds in the radiographs. There were differences in the physical parameters obtained by the semi-automated analysis of the radiographs between the seed lots. Also, the lots differed regarding the physiological quality of the seeds. Morphometric characteristics and tissue integrity, especially for the seed embryo, showed high correlation with the seed physiological quality. Therefore, this work presents an efficient approach to rapid and non-destructively assess the quality of crambe seeds.
Author Medeiros, André Dantas de
Milagres, Carla do Carmo
Neri, Tássia Fernanda Santos
Silva, Laércio Junio da
Ribeiro, Alessandra da Silva
AuthorAffiliation Universidade Federal de Viçosa
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  organization: Universidade Federal de Viçosa, Brazil
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DocumentTitleAlternate Uso de imagens radiográficas para avaliação rápida e não destrutiva da qualidade de sementes de crambe
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Keywords relative density
densidade relativa
Crambe abyssinica
radiografia de sementes
image analysis
seed radiography
análise de imagem
Language English
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Snippet Technologies based on electromagnetic radiation, such as the X-ray technique, has contributed to the establishment of new and promising methodologies for...
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SubjectTerms Crambe abyssinica
image analysis
PLANT SCIENCES
relative density
seed radiography
Title Use of radiographic images for rapid and non-destructive assessment of crambe seed quality
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