Investigation of graded-level soybean meal diets in red drum (Sciaenops ocellatus) using NMR-based metabolomics analysis

• Published in: Comparative biochemistry and physiology. Part D, Genomics & proteomics Vol. 29; pp. 173 - 184
• Main Authors: Casu, Fabio, Watson, Aaron M., Yost, Justin, Leffler, John W., Gaylord, T. Gibson, Barrows, Frederic T., Sandifer, Paul A., Denson, Michael R., Bearden, Daniel W.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.03.2019
• Subjects: Animal Feed; Animals; Aquaculture; Fishes - metabolism; Glycine max; Magnetic Resonance Spectroscopy - methods; Metabolomics; Metabolomics - methods; NMR; Red drum; Sciaenops ocellatus; Soybean meal; Metabolomics; Red drum; Sciaenops ocellatus; NMR; Aquaculture; Soybean meal
• ISSN: 1744-117X; 1878-0407
• DOI: 10.1016/j.cbd.2018.11.009

We investigated changes in the metabolome in juvenile red drum (Sciaenops ocellatus) induced by increasing amounts of soybean meal (0% to 60%) in extruded, fishmeal-free diets using a nuclear magnetic resonance spectroscopy (NMR)-based metabolomics approach in a 12-week feeding trial. All of the diets were composed of ≈40% total crude protein, ≈11% total crude lipid and were energetically balanced. A fishmeal-containing, commercial extruded diet was used as a control diet throughout the trial. Each week, liver, muscle, intestine and plasma samples were collected and analyzed by NMR to provide a “snapshot” of the metabolome at different time points. Results indicate significant time-dependence of the metabolic profiles in various tissues with stable metabolomic profiles forming after about 9-weeks on the experimental diets. We identify a previously unexploited biomarker of potential dietary stress (N‑formimino‑l‑glutamate (FIGLU)) in the fish that may prove to be useful for optimization of alternative diet formulations. [Display omitted]