Rapid differential diagnosis of SARS-CoV-2, influenza A/B and respiratory syncytial viruses: Validation of a novel RT-PCR assay

• Published in: Journal of clinical virology Vol. 161; p. 105402
• Main Authors: Domnich, Alexander, Bruzzone, Bianca, Trombetta, Carlo-Simone, De Pace, Vanessa, Ricucci, Valentina, Varesano, Serena, Garzillo, Giada, Ogliastro, Matilde, Orsi, Andrea, Icardi, Giancarlo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2023
• Subjects: Coinfection - diagnosis; COVID-19 - diagnosis; Diagnosis, Differential; Diagnostic accuracy; Humans; Influenza; Influenza A virus - genetics; Influenza B virus - genetics; Influenza, Human - diagnosis; Molecular Diagnostic Techniques - methods; point-of-care testing; rapid RT-PCR; Real-Time Polymerase Chain Reaction - methods; Respiratory Syncytial Virus Infections - diagnosis; Respiratory Syncytial Virus, Human - genetics; Respiratory Syncytial Viruses; Retrospective Studies; Reverse Transcriptase Polymerase Chain Reaction; RSV; SARS-CoV-2; SARS-CoV-2 - genetics; Sensitivity and Specificity; point-of-care testing; SARS-CoV-2; RSV; Influenza; Diagnostic accuracy; rapid RT-PCR

•STANDARD M10 Flu/RSV/SARS-CoV-2 PoC RT-PCR assay was compared with Allplex RT-PCR.•STANDARD M10 Flu/RSV/SARS-CoV-2 showed high diagnostic performance for all viruses.•PoC RT-PCR assays are useful for differential diagnosis of the common respiratory viruses. Influenza and respiratory syncytial (RSV)...