Selection of green fluorescent proteins by in vitro compartmentalization using microbead-display libraries

• Published in: Biochemical engineering journal Vol. 187; p. 108627
• Main Authors: Iizuka, Ryo, Tahara, Kentaro, Matsueda, Anna, Tsuda, Soichiro, Yoon, Dong Hyun, Sekiguchi, Tetsushi, Shoji, Shuichi, Funatsu, Takashi
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2022
• Subjects: directed evolution; DNA; DNA libraries; emulsions; fluorescence; gene expression; genotype; Green fluorescent protein; In vitro compartmentalization; Microbead display; microbeads; Microdroplet; peptides; phenotype; ribozymes; Microbead display; In vitro compartmentalization; Green fluorescent protein; Microdroplet

In vitro compartmentalization (IVC) is a method to link genotype and phenotype by confining DNA and an in vitro gene expression system in cell-like compartments such as water-in-oil microdroplets. IVC provides a flexible platform for the selection and directed evolution of peptides, proteins, and RN...