Efficient and Non-Toxic Biological Response Carrier Delivering TNF-α shRNA for Gene Silencing in a Murine Model of Rheumatoid Arthritis

Small interfering RNA (siRNA) is an effective and specific method for silencing genes. However, an efficient and non-toxic carrier is needed to deliver the siRNA into the target cells. Tumor necrosis factor α (TNF-α) plays a central role in the occurrence and progression of rheumatoid arthritis (RA)...

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Published inFrontiers in immunology Vol. 7; p. 305
Main Authors Song, Jialin, Chen, Yinghui, Jiang, Shichao, Yang, Kejia, Li, Xiaoming, Zhao, Xiaotian, Ouyang, Yuanming, Fan, Cunyi, Yuan, Weien
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 19.08.2016
Subjects
Immunology
RNA interference
TNF-α
rheumatoid arthritis
PDAPEI
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Abstract Small interfering RNA (siRNA) is an effective and specific method for silencing genes. However, an efficient and non-toxic carrier is needed to deliver the siRNA into the target cells. Tumor necrosis factor α (TNF-α) plays a central role in the occurrence and progression of rheumatoid arthritis (RA). In this study, we pre-synthetized a degradable cationic polymer (PDAPEI) from 2,6-pyridinedicarboxaldehyde and low-molecular-weight polyethyleneimine (PEI, Mw = 1.8 kDa) as a gene vector for the delivery of TNF-α shRNA. The PDAPEI/pDNA complex showed a suitable particle size and stable zeta potential for transfection. In vitro study of the PDAPEI/pDNA complex revealed a lower cytotoxicity and higher transfection efficiency when transfecting TNF-α shRNA to macrophages by significantly down-regulating the expression of TNF-α. Moreover, the complex was extremely efficient in decreasing the severity of arthritis in mice with collagen-induced arthritis. PDAPEI delivered TNF-α shRNA has great potential in the treatment of RA.
AbstractList Small interfering RNA (siRNA) is an effective and specific method for silencing genes. However, an efficient and non-toxic carrier is needed to deliver the siRNA into the target cells. Tumor necrosis factor α (TNF-α) plays a central role in the occurrence and progression of rheumatoid arthritis (RA). In this study, we pre-synthetized a degradable cationic polymer (PDAPEI) from 2,6-pyridinedicarboxaldehyde and low-molecular-weight polyethyleneimine (PEI, Mw = 1.8 kDa) as a gene vector for the delivery of TNF-α shRNA. The PDAPEI/pDNA complex showed a suitable particle size and stable zeta potential for transfection. In vitro study of the PDAPEI/pDNA complex revealed a lower cytotoxicity and higher transfection efficiency when transfecting TNF-α shRNA to macrophages by significantly down-regulating the expression of TNF-α. Moreover, the complex was extremely efficient in decreasing the severity of arthritis in mice with collagen-induced arthritis. PDAPEI delivered TNF-α shRNA has great potential in the treatment of RA.
Small interfering RNA (siRNA) is an effective and specific method for silencing genes. However, an efficient and non-toxic carrier is needed to deliver the siRNA into the target cells. Tumor necrosis factor α (TNF-α) plays a central role in the occurrence and progression of rheumatoid arthritis (RA). In this study, we pre-synthetized a degradable cationic polymer (PDAPEI) from 2,6-pyridinedicarboxaldehyde and low-molecular-weight polyethyleneimine (PEI, Mw = 1.8 kDa) as a gene vector for the delivery of TNF-α shRNA. The PDAPEI/pDNA complex showed a suitable particle size and stable zeta potential for transfection. In vitro study of the PDAPEI/pDNA complex revealed a lower cytotoxicity and higher transfection efficiency when transfecting TNF-α shRNA to macrophages by significantly down-regulating the expression of TNF-α. Moreover, the complex was extremely efficient in decreasing the severity of arthritis in mice with collagen-induced arthritis. PDAPEI delivered TNF-α shRNA has great potential in the treatment of RA.
Author Yang, Kejia
Li, Xiaoming
Song, Jialin
Zhao, Xiaotian
Chen, Yinghui
Ouyang, Yuanming
Fan, Cunyi
Jiang, Shichao
Yuan, Weien
AuthorAffiliation 5 Shanghai Sixth People’s Hospital East Campus, Shanghai University of Medicine and Health , Shanghai , China
1 Shanghai Jiao Tong University Affiliated Sixth People’s Hospital , Shanghai , China
4 School of Pharmacy, Shanghai JiaoTong University , Shanghai , China
3 Department of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University , Jinan, Shandong , China
2 Department of Neurology, Jinshan Hospital, Fudan University , Shanghai , China
AuthorAffiliation_xml – name: 4 School of Pharmacy, Shanghai JiaoTong University , Shanghai , China
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– name: 3 Department of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University , Jinan, Shandong , China
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Cites_doi 10.1136/ard.2010.134684
10.1016/j.cyto.2010.10.010
10.1093/rheumatology/kes304
10.1016/j.ejpb.2004.11.011
10.1093/rheumatology/kev422
10.1038/nature07756
10.1517/14712590902926089
10.1208/s12248-009-9143-y
10.7860/JCDR/2015/10443.5394
10.1038/mt.2013.245
10.3389/fimmu.2015.00384
10.1016/S1473-3099(03)00545-0
10.1016/j.jconrel.2004.11.008
10.1002/art.27503
10.1016/j.biomaterials.2011.12.003
10.1007/s11095-007-9287-3
10.1016/j.biomaterials.2012.08.008
10.1016/j.biomaterials.2012.05.044
10.1046/j.1365-2249.1996.d01-820.x
10.1007/s00253-003-1321-8
10.1016/j.biomaterials.2008.06.003
10.1038/nature07758
10.1002/art.23280
10.1136/ard.2008.091264
10.1016/j.joca.2011.01.012
10.2174/187152812800392706
10.1007/s10165-010-0277-7
10.1038/nrd3625
10.1038/mt.2011.156
10.1056/NEJMra1004965
10.1016/S0140-6736(07)60784-3
10.1002/art.24106
10.1016/S0378-1119(00)00224-9
10.1016/j.jconrel.2015.04.014
10.1016/j.jconrel.2008.07.007
10.1007/978-1-60761-588-0_1
10.1186/1477-3155-12-13
10.1172/JCI36389
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Keywords RNA interference
TNF-α
rheumatoid arthritis
PDAPEI
Language English
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Edited by: Claudio Mauro, Queen Mary University of London, UK
Reviewed by: Sian M. Henson, Queen Mary University of London, UK; Jane Falconer, University of Birmingham, UK
Specialty section: This article was submitted to Inflammation, a section of the journal Frontiers in Immunology
Jialin Song and Yinghui Chen contributed equally to this work.
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References 20195684 - Mod Rheumatol. 2010 Aug;20(4):325-30
18821658 - Arthritis Rheum. 2008 Oct 15;59(10):1467-74
15939236 - Eur J Pharm Biopharm. 2005 Jul;60(2):247-66
18801760 - Ann Rheum Dis. 2009 Jul;68(7):1171-6
18311805 - Arthritis Rheum. 2008 Mar;58(3):637-40
22262036 - Nat Rev Drug Discov. 2012 Jan 20;11(2):125-40
21111629 - Cytokine. 2011 Feb;53(2):207-14
22280240 - Inflamm Allergy Drug Targets. 2012 Jun;11(3):188-95
22910222 - Biomaterials. 2012 Nov;33(33):8600-12
18982160 - J Clin Invest. 2008 Nov;118(11):3537-45
24145554 - Mol Ther. 2014 Feb;22(2):397-408
19158787 - Nature. 2009 Jan 22;457(7228):413-20
22192536 - Biomaterials. 2012 Mar;33(8):2570-82
20506322 - Arthritis Rheum. 2010 Aug;62(8):2192-205
19158789 - Nature. 2009 Jan 22;457(7228):426-33
21289422 - Clin Calcium. 2011 Feb;21(2):253-9
8870692 - Clin Exp Immunol. 1996 Oct;106(1):13-9
21811249 - Mol Ther. 2011 Oct;19(10):1887-95
20959326 - Ann Rheum Dis. 2010 Nov;69(11):1898-906
26284069 - Front Immunol. 2015 Jul 27;6:384
18692533 - J Control Release. 2008 Oct 6;131(1):70-6
22695070 - Biomaterials. 2012 Sep;33(27):6520-32
25886704 - J Control Release. 2015 Jun 10;207:143-53
17385010 - Pharm Res. 2007 Aug;24(8):1590-8
23204551 - Rheumatology (Oxford). 2013 Apr;52(4):590-8
10903441 - Gene. 2000 Jul 11;252(1-2):95-105
20217541 - Methods Mol Biol. 2010;623:3-19
24708586 - J Nanobiotechnology. 2014 Apr 06;12:13
18585778 - Biomaterials. 2008 Oct;29(28):3872-81
19392574 - Expert Opin Biol Ther. 2009 May;9(5):535-8
15710512 - J Control Release. 2005 Mar 2;103(1):209-19
25738007 - J Clin Diagn Res. 2015 Jan;9(1):GE01-6
12614731 - Lancet Infect Dis. 2003 Mar;3(3):148-55
17570481 - Lancet. 2007 Dec 1;370(9602):1861-74
21262368 - Osteoarthritis Cartilage. 2011 Apr;19(4):458-65
19834816 - AAPS J. 2009 Dec;11(4):671-81
26715774 - Rheumatology (Oxford). 2016 May;55(5):928-38
12719940 - Appl Microbiol Biotechnol. 2003 Jul;62(1):27-34
22150039 - N Engl J Med. 2011 Dec 8;365(23):2205-19
Scholtysek (B3) 2012; 11
Park (B22) 2012; 33
Ko (B32) 2008; 29
Thomas (B21) 2003; 62
Lioté (B5) 1996; 106
Gabriel (B8) 2008; 58
Bevaart (B38) 2010; 62
Nam (B7) 2010; 20
Young Heui (B26) 2005; 103
Bejarano (B9) 2008; 59
Smolen (B35) 2007; 370
Ge (B34) 2014; 12
Mellado (B37) 2015; 6
Govindarajan (B12) 2012; 33
Moazed (B13) 2009; 457
Courties (B18) 2009; 9
Choi (B24) 2008; 131
Xiang (B25) 2012; 33
Hoff (B10) 2009; 68
Castanotto (B14) 2009; 457
Lungwitz (B23) 2005; 60
McInnes (B1) 2010; 69
Sidahmed (B28) 2010; 623
Davignon (B17) 2013; 52
van der Aa (B31) 2007; 24
Ghassemi-Nejad (B2) 2011; 19
Choe (B15) 2016; 55
Al-Dosari (B20) 2009; 11
Kole (B29) 2012; 11
Caplen (B30) 2000; 252
McInnes (B6) 2011; 365
Ramamoorth (B19) 2015; 9
Calmon-Hamaty (B4) 2011; 53
Lee (B16) 2014; 22
Gardam (B39) 2003; 3
Paquet (B11) 2011; 19
Layek (B33) 2015; 207
Brennan (B36) 2008; 118
Hirose (B27) 2011; 21
References_xml – volume: 69
  start-page: 1898
  year: 2010
  ident: B1
  article-title: State-of-the-art: rheumatoid arthritis
  publication-title: Ann Rheum Dis
  doi: 10.1136/ard.2010.134684
  contributor:
    fullname: McInnes
– volume: 53
  start-page: 207
  year: 2011
  ident: B4
  article-title: Lymphotoxin α stimulates proliferation and pro-inflammatory cytokine secretion of rheumatoid arthritis synovial fibroblasts
  publication-title: Cytokine
  doi: 10.1016/j.cyto.2010.10.010
  contributor:
    fullname: Calmon-Hamaty
– volume: 52
  start-page: 590
  year: 2013
  ident: B17
  article-title: Targeting monocytes/macrophages in the treatment of rheumatoid arthritis
  publication-title: Rheumatology
  doi: 10.1093/rheumatology/kes304
  contributor:
    fullname: Davignon
– volume: 60
  start-page: 247
  year: 2005
  ident: B23
  article-title: Polyethylenimine-based non-viral gene delivery systems
  publication-title: Eur J Pharm Biopharm
  doi: 10.1016/j.ejpb.2004.11.011
  contributor:
    fullname: Lungwitz
– volume: 55
  start-page: 928
  issue: 5
  year: 2016
  ident: B15
  article-title: Activation of dickkopf-1 and focal adhesion kinase pathway by tumour necrosis factor α induces enhanced migration of fibroblast-like synoviocytes in rheumatoid arthritis
  publication-title: Rheumatology (Oxford)
  doi: 10.1093/rheumatology/kev422
  contributor:
    fullname: Choe
– volume: 457
  start-page: 413
  year: 2009
  ident: B13
  article-title: Small RNAs in transcriptional gene silencing and genome defence
  publication-title: Nature
  doi: 10.1038/nature07756
  contributor:
    fullname: Moazed
– volume: 9
  start-page: 535
  year: 2009
  ident: B18
  article-title: RNA interference-based gene therapy for successful treatment of rheumatoid arthritis
  publication-title: Expert Opin Biol Ther
  doi: 10.1517/14712590902926089
  contributor:
    fullname: Courties
– volume: 11
  start-page: 671
  year: 2009
  ident: B20
  article-title: Nonviral gene delivery: principle, limitations, and recent progress
  publication-title: AAPS J
  doi: 10.1208/s12248-009-9143-y
  contributor:
    fullname: Al-Dosari
– volume: 9
  start-page: GE01
  year: 2015
  ident: B19
  article-title: Non viral vectors in gene therapy- an overview
  publication-title: J Clin Diagn Res
  doi: 10.7860/JCDR/2015/10443.5394
  contributor:
    fullname: Ramamoorth
– volume: 22
  start-page: 397
  year: 2014
  ident: B16
  article-title: TNF-alpha gene silencing using polymerized siRNA/thiolated glycol chitosan nanoparticles for rheumatoid arthritis
  publication-title: Mol Ther
  doi: 10.1038/mt.2013.245
  contributor:
    fullname: Lee
– volume: 6
  start-page: 384
  year: 2015
  ident: B37
  article-title: T cell migration in rheumatoid arthritis
  publication-title: Front Immunol
  doi: 10.3389/fimmu.2015.00384
  contributor:
    fullname: Mellado
– volume: 3
  start-page: 148
  year: 2003
  ident: B39
  article-title: Anti-tumour necrosis factor agents and tuberculosis risk: mechanisms of action and clinical management
  publication-title: Lancet Infect Dis
  doi: 10.1016/S1473-3099(03)00545-0
  contributor:
    fullname: Gardam
– volume: 103
  start-page: 209
  year: 2005
  ident: B26
  article-title: Polyethylenimine with acid-labile linkages as a biodegradable gene carrier
  publication-title: J Control Release
  doi: 10.1016/j.jconrel.2004.11.008
  contributor:
    fullname: Young Heui
– volume: 62
  start-page: 2192
  year: 2010
  ident: B38
  article-title: Evaluation of therapeutic targets in animal models of arthritis: how does it relate to rheumatoid arthritis?
  publication-title: Arthritis Rheumatol
  doi: 10.1002/art.27503
  contributor:
    fullname: Bevaart
– volume: 33
  start-page: 2570
  year: 2012
  ident: B12
  article-title: Targeting human epidermal growth factor receptor 2 by a cell-penetrating peptide–affibody bioconjugate
  publication-title: Biomaterials
  doi: 10.1016/j.biomaterials.2011.12.003
  contributor:
    fullname: Govindarajan
– volume: 24
  start-page: 1590
  year: 2007
  ident: B31
  article-title: Cellular uptake of cationic polymer-DNA complexes via caveolae plays a pivotal role in gene transfection in COS-7 Cells
  publication-title: Pharm Res
  doi: 10.1007/s11095-007-9287-3
  contributor:
    fullname: van der Aa
– volume: 33
  start-page: 8600
  year: 2012
  ident: B22
  article-title: The use of anti-COX2 siRNA coated onto PLGA nanoparticles loading dexamethasone in the treatment of rheumatoid arthritis
  publication-title: Biomaterials
  doi: 10.1016/j.biomaterials.2012.08.008
  contributor:
    fullname: Park
– volume: 33
  start-page: 6520
  year: 2012
  ident: B25
  article-title: Biscarbamate cross-linked low molecular weight PEI for delivering IL-1 receptor antagonist gene to synoviocytes for arthritis therapy
  publication-title: Biomaterials
  doi: 10.1016/j.biomaterials.2012.05.044
  contributor:
    fullname: Xiang
– volume: 106
  start-page: 13
  year: 1996
  ident: B5
  article-title: Blood monocyte activation in rheumatoid arthritis: increased monocyte adhesiveness, integrin expression, and cytokine release
  publication-title: Clin Exp Immunol
  doi: 10.1046/j.1365-2249.1996.d01-820.x
  contributor:
    fullname: Lioté
– volume: 62
  start-page: 27
  year: 2003
  ident: B21
  article-title: Non-viral gene therapy: polycation-mediated DNA delivery
  publication-title: Appl Microbiol Biotechnol
  doi: 10.1007/s00253-003-1321-8
  contributor:
    fullname: Thomas
– volume: 29
  start-page: 3872
  year: 2008
  ident: B32
  article-title: Acid-degradable cationic methacrylamide polymerized in the presence of plasmid DNA as tunable non-viral gene carrier
  publication-title: Biomaterials
  doi: 10.1016/j.biomaterials.2008.06.003
  contributor:
    fullname: Ko
– volume: 457
  start-page: 426
  year: 2009
  ident: B14
  article-title: The promises and pitfalls of RNA-interference-based therapeutics
  publication-title: Nature
  doi: 10.1038/nature07758
  contributor:
    fullname: Castanotto
– volume: 58
  start-page: 637
  year: 2008
  ident: B8
  article-title: Tumor necrosis factor inhibition: a part of the solution or a part of the problem of heart failure in rheumatoid arthritis?
  publication-title: Arthritis Rheum
  doi: 10.1002/art.23280
  contributor:
    fullname: Gabriel
– volume: 68
  start-page: 1171
  year: 2009
  ident: B10
  article-title: Adalimumab therapy reduces hand bone loss in early rheumatoid arthritis: explorative analyses from the PREMIER study
  publication-title: Ann Rheum Dis
  doi: 10.1136/ard.2008.091264
  contributor:
    fullname: Hoff
– volume: 19
  start-page: 458
  year: 2011
  ident: B2
  article-title: Osteoarthritis-like damage of cartilage in the temporomandibular joints in mice with autoimmune inflammatory arthritis
  publication-title: Osteoarthritis Cartilage
  doi: 10.1016/j.joca.2011.01.012
  contributor:
    fullname: Ghassemi-Nejad
– volume: 11
  start-page: 188
  year: 2012
  ident: B3
  article-title: Inflammation-associated changes in bone homeostasis
  publication-title: Inflamm Allergy Drug Targets
  doi: 10.2174/187152812800392706
  contributor:
    fullname: Scholtysek
– volume: 20
  start-page: 325
  year: 2010
  ident: B7
  article-title: Aspects of TNF inhibitor therapy in rheumatoid arthritis
  publication-title: Mod Rheumatol
  doi: 10.1007/s10165-010-0277-7
  contributor:
    fullname: Nam
– volume: 11
  start-page: 125
  year: 2012
  ident: B29
  article-title: RNA therapeutics: beyond RNA interference and antisense oligonucleotides
  publication-title: Nat Rev Drug Discov
  doi: 10.1038/nrd3625
  contributor:
    fullname: Kole
– volume: 19
  start-page: 1887
  year: 2011
  ident: B11
  article-title: Alternative for anti-TNF antibodies for arthritis treatment
  publication-title: Br J Educ Technol
  doi: 10.1038/mt.2011.156
  contributor:
    fullname: Paquet
– volume: 365
  start-page: 2205
  year: 2011
  ident: B6
  article-title: The pathogenesis of rheumatoid arthritis
  publication-title: N Engl J Med
  doi: 10.1056/NEJMra1004965
  contributor:
    fullname: McInnes
– volume: 370
  start-page: 1861
  year: 2007
  ident: B35
  article-title: New therapies for treatment of rheumatoid arthritis
  publication-title: Lancet
  doi: 10.1016/S0140-6736(07)60784-3
  contributor:
    fullname: Smolen
– volume: 21
  start-page: 253
  year: 2011
  ident: B27
  article-title: [Animal models for bone and joint disease. CIA, CAIA model]
  publication-title: Clin Calcium
  contributor:
    fullname: Hirose
– volume: 59
  start-page: 1467
  year: 2008
  ident: B9
  article-title: Effect of the early use of the anti–tumor necrosis factor adalimumab on the prevention of job loss in patients with early rheumatoid arthritis
  publication-title: Arthritis Rheum
  doi: 10.1002/art.24106
  contributor:
    fullname: Bejarano
– volume: 252
  start-page: 95
  year: 2000
  ident: B30
  article-title: dsRNA-mediated gene silencing in cultured Drosophila cells: a tissue culture model for the analysis of RNA interference
  publication-title: Gene
  doi: 10.1016/S0378-1119(00)00224-9
  contributor:
    fullname: Caplen
– volume: 207
  start-page: 143
  year: 2015
  ident: B33
  article-title: APC targeted micelle for enhanced intradermal delivery of hepatitis B DNA vaccine
  publication-title: J Control Release
  doi: 10.1016/j.jconrel.2015.04.014
  contributor:
    fullname: Layek
– volume: 131
  start-page: 70
  year: 2008
  ident: B24
  article-title: Enhanced gene delivery using disulfide-crosslinked low molecular weight polyethylenimine with listeriolysin o-polyethylenimine disulfide conjugate
  publication-title: J Control Release
  doi: 10.1016/j.jconrel.2008.07.007
  contributor:
    fullname: Choi
– volume: 623
  start-page: 3
  year: 2010
  ident: B28
  article-title: Endogenous antiviral mechanisms of RNA interference: a comparative biology perspective
  publication-title: Methods Mol Biol
  doi: 10.1007/978-1-60761-588-0_1
  contributor:
    fullname: Sidahmed
– volume: 12
  start-page: 13
  year: 2014
  ident: B34
  article-title: Biscarbamate cross-linked low molecular weight polyethylenimine polycation as an efficient intra-cellular delivery cargo for cancer therapy
  publication-title: J Nanobiotechnology
  doi: 10.1186/1477-3155-12-13
  contributor:
    fullname: Ge
– volume: 118
  start-page: 3537
  year: 2008
  ident: B36
  article-title: Evidence that cytokines play a role in rheumatoid arthritis
  publication-title: J Clin Invest
  doi: 10.1172/JCI36389
  contributor:
    fullname: Brennan
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Snippet Small interfering RNA (siRNA) is an effective and specific method for silencing genes. However, an efficient and non-toxic carrier is needed to deliver the...
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Title Efficient and Non-Toxic Biological Response Carrier Delivering TNF-α shRNA for Gene Silencing in a Murine Model of Rheumatoid Arthritis
URI https://www.ncbi.nlm.nih.gov/pubmed/27594856
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