Influence of cytosolic conditions on the reaction equilibrium and the reaction enthalpy of the enolase reaction accessed by calorimetry and van ‘t HOFF

• Published in: Biochimica et biophysica acta. General subjects Vol. 1864; no. 10; p. 129675
• Main Authors: Vogel, Kristina, Greinert, Thorsten, Harms, Hauke, Sadowski, Gabriele, Held, Christoph, Maskow, Thomas
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2020
• Subjects: Biothermodynamics; Calorimetry; Cytosol - metabolism; electrolytes; Enolase; enthalpy; ePC-SAFT; equations; Gibbs free energy; Glycolysis; Isothermal titration calorimetry (ITC); Macromolecular crowding; phosphopyruvate hydratase; Phosphopyruvate Hydratase - metabolism; polyethylene glycol; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - metabolism; salts; systems biology; temperature; Thermodynamics; Macromolecular crowding; Glycolysis; Isothermal titration calorimetry (ITC); Enolase; Biothermodynamics; ePC-SAFT

Thermodynamic methods are finding more and more applications in systems biology, which attempts to understand cell functions mechanistically. Unfortunately, the state variables used (reaction enthalpy and Gibbs energy) do not take sufficient account of the conditions inside of cells, especially the...