Mechanistic studies for the role of cellular nucleic-acid-binding protein (CNBP) in regulation of c-myc transcription

• Published in: Biochimica et biophysica acta Vol. 1830; no. 10; pp. 4769 - 4777
• Main Authors: Chen, Siqi, Su, Lijuan, Qiu, Jun, Xiao, Nannan, Lin, Jing, Tan, Jia-heng, Ou, Tian-miao, Gu, Lian-quan, Huang, Zhi-shu, Li, Ding
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2013
• Subjects: Amino Acid Sequence; Base Sequence; c-myc; Cellular nucleic-acid-binding protein; Chromatin Immunoprecipitation; Circular Dichroism; DNA Primers; Electrophoretic Mobility Shift Assay; Fluorescence Resonance Energy Transfer; G-quadruplex; Genes, myc; Humans; luciferase; Molecular Sequence Data; NM23 Nucleoside Diphosphate Kinases - metabolism; Nuclease hypersensitive element III1; protein-protein interactions; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; RNA-Binding Proteins - chemistry; RNA-Binding Proteins - metabolism; RNA-Binding Proteins - physiology; Sequence Homology, Amino Acid; Surface Plasmon Resonance; transcription (genetics); transcription factors; Transcription, Genetic; Transcriptional Activation; Transcriptional regulation; Nuclease hypersensitive element III1; CD; Cellular nucleic-acid-binding protein; c-myc; CNBP; G-quadruplex; NHE; Co-IP; ChIP; Transcriptional regulation; TAMRA; FAM; SPR; EMSA; FRET; TMPyP4; RT-qPCR; meso-tetra(N-methyl-4-pyridyl)porphine; circular dichroism; co-immunoprecipitation; chromatin immunoprecipitation; electrophoretic mobility shift assay; surface plasmon resonance; 6-carboxyfluorescein; reverse transcription-quantitative polymerase chain reaction; nuclease hypersensitive element; fluorescence resonance energy transfer; tetramethylrhodamine; Nuclease hypersensitive element III

Guanine-rich sequence of c-myc nuclease hypersensitive element (NHE) III1 is known to fold in G-quadruplex and subsequently serves as a transcriptional silencer. Cellular nucleic-acid-binding protein (CNBP), a highly conserved zinc-finger protein with multiple biological functions, could bind to c-m...