Single-molecule imaging and manipulation of biomolecular machines and systems

• Published in: Biochimica et biophysica acta Vol. 1862; no. 2; pp. 241 - 252
• Main Authors: Iino, Ryota, Iida, Tatsuya, Nakamura, Akihiko, Saita, Ei-ichiro, You, Huijuan, Sako, Yasushi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2018
• Subjects: energy; image analysis; mechanical properties; Molecular machines; molecular motor proteins; Motor proteins; Signal transduction; Single-molecule imaging; Single-molecule manipulation; Molecular machines; iSCAT; Single-molecule imaging; STED; AFM; Single-molecule manipulation; ADP; G4; Motor proteins; GFP; Signal transduction; S/N; FCS; ICS; Pi; FRET; TIR; ATP

Biological molecular machines support various activities and behaviors of cells, such as energy production, signal transduction, growth, differentiation, and migration. We provide an overview of single-molecule imaging methods involving both small and large probes used to monitor the dynamic motions...