Gene network analysis of oxaliplatin-resistant colorectal cancer to target a crucial gene using chitosan/hyaluronic acid/protamine polyplexes containing CRISPR-Cas9

• Published in: Biochimica et biophysica acta. General subjects Vol. 1867; no. 8; p. 130385
• Main Authors: Shirani-Bidabadi, Shiva, Mirian, Mina, Varshosaz, Jaleh, Tavazohi, Nazita, Sadeghi, Hamid Mir Mohammad, Shariati, Laleh
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2023
• Subjects: Chitosan; Colorectal cancer; colorectal neoplasms; Colorectal Neoplasms - drug therapy; Colorectal Neoplasms - genetics; CRISPR-Cas systems; CRISPR-Cas Systems - genetics; CRISPR/Cas9; DNA repair; Drug resistance; excision; fluorouracil; Gene Regulatory Networks; genes; Humans; Hyaluronic Acid; Oxaliplatin; Oxaliplatin - pharmacology; Oxaliplatin - therapeutic use; particle size; plasmids; Polyplex; Protamines - genetics; Protamines - therapeutic use; sulfates; toxicity; transfection; zeta potential; Polyplex; Oxaliplatin; CRISPR/Cas9; Drug resistance; Colorectal cancer

Colorectal cancer (CRC) treatment is dramatically hampered by resistance to oxaliplatin alone or in the combination of irinotecan or 5-fluorouracil and leucovorin. This study aims to design and assess Chitosan/Hyaluronic Acid/Protamine sulfate (CS/HA/PS) polyplexes loaded with CRISPR plasmid for tar...