Multi-level evaluation of Escherichia coli polyphosphate related mutants using global transcriptomic, proteomic and phenomic analyses

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 4; pp. 871 - 883
• Main Authors: Varas, Macarena, Valdivieso, Camilo, Mauriaca, Cecilia, Ortíz-Severín, Javiera, Paradela, Alberto, Poblete-Castro, Ignacio, Cabrera, Ricardo, Chávez, Francisco P.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2017
• Subjects: anti-infective agents; Antitoxins - genetics; bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; biochemical pathways; bioinformatics; biopolymers; Cell Respiration - genetics; energy; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli Proteins - genetics; fermentation; Fermentation - genetics; gene expression regulation; Metabolic Networks and Pathways - genetics; microarray technology; mutants; Mutation - genetics; Omics; pathogens; phenotype; Phenotypic microarray; Phosphotransferases (Alcohol Group Acceptor) - genetics; PolyP-dependent proteolysis; Polyphosphate; Polyphosphates - metabolism; proteolysis; proteome; Proteome - genetics; proteomics; Proteomics - methods; Q-proteomics; Sigma Factor - genetics; Systems Biology; transcriptome; Transcriptome - genetics; transcriptomics; Up-Regulation - genetics; Q-proteomics; Phenotypic microarray; Polyphosphate; Systems Biology; PolyP-dependent proteolysis; Omics

Polyphosphate (polyP) is a linear biopolymer found in all living cells. In bacteria, mutants lacking polyphosphate kinase 1 (PPK1), the enzyme responsible for synthesis of most polyP, have many structural and functional defects. However, little is known about the causes of these pleiotropic alterati...