Three-sectioning method: A procedure for studying hard tissues and large pieces under light and electron microscopy

• Published in: Micron (Oxford, England : 1993) Vol. 132; p. 102841
• Main Authors: Gayoso, Jorge, Garrosa, Manuel, Gayoso, Sara, Rodríguez-Arias, Carlos Alberto, Martin-Ferrero, Miguel Ángel, Gayoso, Manuel José
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2020
• Subjects: Bone; epoxides; Epoxy resin; Hard tissues; histology; light microscopy; Nerve; Plastic embedding; Prostheses; scanning electron microscopy; Sectioning; SEM; Semithin sections; TEM; tendons; Thick sections; transmission electron microscopy; Ultrathin sections; Nerve; Semithin sections; Prostheses; Hard tissues; Epoxy resin; SEM; Bone; Plastic embedding; Ultrathin sections; TEM; Sectioning; Thick sections

•The serial sections are easy to manipulate as a whole and artifact-free.•Suitable to study the interphase prostheses-tissues.•To cut areas with different hardness without causing splintering or wrinkling.•The avoidance of grinding saves most of the original piece.•Useful for stereo, light and elect...