Identification, characterization, immobilization, and mutational analysis of a novel acetylesterase with industrial potential (LaAcE) from Lactobacillus acidophilus

• Published in: Biochimica et biophysica acta. General subjects Vol. 1862; no. 1; pp. 197 - 210
• Main Authors: Wang, Ying, Ryu, Bum Han, Yoo, Wanki, Lee, Chang Woo, Kim, Kyeong Kyu, Lee, Jun Hyuck, Kim, T. Doohun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2018
• Subjects: acetic acid; Acetylesterase; Acetylesterase - chemistry; Acetylesterase - genetics; Amino Acid Substitution; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; dairy products; enzyme kinetics; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - genetics; fermentation; fish oils; glucose; hydrophobicity; industrial applications; LaAcE; lactic acid bacteria; Lactobacillus acidophilus; Lactobacillus acidophilus - enzymology; Lactobacillus acidophilus - genetics; meat; Models, Molecular; mutants; mutation; Mutation, Missense; mutational analysis; peptides; polyacrylamide gel electrophoresis; spectroscopy; substrate specificity; Substrate Specificity - genetics; thermal stability; vegetables; Lactobacillus acidophilus; LaAcE; Acetylesterase

Lactic acid bacteria, which are involved in the fermentation of vegetables, meats, and dairy products, are widely used for the productions of small organic molecules and bioactive peptides. Here, a novel acetylesterase (LaAcE) from Lactobacillus acidophilus NCFM was identified, functionally characte...