Imaging extracelluar vesicles by transmission electron microscopy: Coping with technical hurdles and morphological interpretation
Extracellular vesicles (EVs) are cell-derived nanometric particles governing the complex interactions among cells through their bioactive cargo. Interest in EVs is rapidly increasing due to their extensive involvement in physiological and pathological conditions, their potential employment as diagno...
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| Published in | Biochimica et biophysica acta. General subjects Vol. 1865; no. 4; p. 129648 |
|---|---|
| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
Elsevier B.V
01.04.2021
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0304-4165 1872-8006 1872-8006 |
| DOI | 10.1016/j.bbagen.2020.129648 |
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| Abstract | Extracellular vesicles (EVs) are cell-derived nanometric particles governing the complex interactions among cells through their bioactive cargo. Interest in EVs is rapidly increasing due to their extensive involvement in physiological and pathological conditions, their potential employment as diagnostic and therapeutic tools and their prospective use as bio-carriers of exogenous molecules. Given their nanometric size, transmission electron microscopy (TEM) provides significant contributions to assess EV presence and purity in a sample and to study morphological features.
In this review, TEM methods for EV imaging are compared with respect to their applications, benefits and drawbacks. A critical evaluation of the actual contribution of TEM to the study of EVs is also provided and the most common artifacts encountered in the literature are discussed.
TEM techniques are powerful tools for the investigation of EVs and have the potential to reveal sample purity, ultrastructure and molecular composition. However, technical challenges, procedural errors in sample processing or misinterpretations may result in a variety of different morphologies and artifacts.
The last decades have seen exponential technological progress in EV imaging by TEM. Nevertheless, protocols have not been standardized yet and sample preparation remains a critical step. An optimized, standardized and integrated protocol of different techniques could minimize artifacts and interpretative errors that could significantly improve the quality and reliability of downstream studies.
•TEM techniques for EV imaging are summarized and compared with respect to their applications, benefits and drawbacks.•TEM contribution to the study of physical-chemical features of EVs is highlighted.•Artifacts commonly encountered in the literature, technical challenges and misinterpretations are discussed. |
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| AbstractList | Extracellular vesicles (EVs) are cell-derived nanometric particles governing the complex interactions among cells through their bioactive cargo. Interest in EVs is rapidly increasing due to their extensive involvement in physiological and pathological conditions, their potential employment as diagnostic and therapeutic tools and their prospective use as bio-carriers of exogenous molecules. Given their nanometric size, transmission electron microscopy (TEM) provides significant contributions to assess EV presence and purity in a sample and to study morphological features.
In this review, TEM methods for EV imaging are compared with respect to their applications, benefits and drawbacks. A critical evaluation of the actual contribution of TEM to the study of EVs is also provided and the most common artifacts encountered in the literature are discussed.
TEM techniques are powerful tools for the investigation of EVs and have the potential to reveal sample purity, ultrastructure and molecular composition. However, technical challenges, procedural errors in sample processing or misinterpretations may result in a variety of different morphologies and artifacts.
The last decades have seen exponential technological progress in EV imaging by TEM. Nevertheless, protocols have not been standardized yet and sample preparation remains a critical step. An optimized, standardized and integrated protocol of different techniques could minimize artifacts and interpretative errors that could significantly improve the quality and reliability of downstream studies. Extracellular vesicles (EVs) are cell-derived nanometric particles governing the complex interactions among cells through their bioactive cargo. Interest in EVs is rapidly increasing due to their extensive involvement in physiological and pathological conditions, their potential employment as diagnostic and therapeutic tools and their prospective use as bio-carriers of exogenous molecules. Given their nanometric size, transmission electron microscopy (TEM) provides significant contributions to assess EV presence and purity in a sample and to study morphological features. In this review, TEM methods for EV imaging are compared with respect to their applications, benefits and drawbacks. A critical evaluation of the actual contribution of TEM to the study of EVs is also provided and the most common artifacts encountered in the literature are discussed. TEM techniques are powerful tools for the investigation of EVs and have the potential to reveal sample purity, ultrastructure and molecular composition. However, technical challenges, procedural errors in sample processing or misinterpretations may result in a variety of different morphologies and artifacts. The last decades have seen exponential technological progress in EV imaging by TEM. Nevertheless, protocols have not been standardized yet and sample preparation remains a critical step. An optimized, standardized and integrated protocol of different techniques could minimize artifacts and interpretative errors that could significantly improve the quality and reliability of downstream studies. •TEM techniques for EV imaging are summarized and compared with respect to their applications, benefits and drawbacks.•TEM contribution to the study of physical-chemical features of EVs is highlighted.•Artifacts commonly encountered in the literature, technical challenges and misinterpretations are discussed. Extracellular vesicles (EVs) are cell-derived nanometric particles governing the complex interactions among cells through their bioactive cargo. Interest in EVs is rapidly increasing due to their extensive involvement in physiological and pathological conditions, their potential employment as diagnostic and therapeutic tools and their prospective use as bio-carriers of exogenous molecules. Given their nanometric size, transmission electron microscopy (TEM) provides significant contributions to assess EV presence and purity in a sample and to study morphological features.BACKGROUNDExtracellular vesicles (EVs) are cell-derived nanometric particles governing the complex interactions among cells through their bioactive cargo. Interest in EVs is rapidly increasing due to their extensive involvement in physiological and pathological conditions, their potential employment as diagnostic and therapeutic tools and their prospective use as bio-carriers of exogenous molecules. Given their nanometric size, transmission electron microscopy (TEM) provides significant contributions to assess EV presence and purity in a sample and to study morphological features.In this review, TEM methods for EV imaging are compared with respect to their applications, benefits and drawbacks. A critical evaluation of the actual contribution of TEM to the study of EVs is also provided and the most common artifacts encountered in the literature are discussed.SCOPE OF REVIEWIn this review, TEM methods for EV imaging are compared with respect to their applications, benefits and drawbacks. A critical evaluation of the actual contribution of TEM to the study of EVs is also provided and the most common artifacts encountered in the literature are discussed.TEM techniques are powerful tools for the investigation of EVs and have the potential to reveal sample purity, ultrastructure and molecular composition. However, technical challenges, procedural errors in sample processing or misinterpretations may result in a variety of different morphologies and artifacts.MAJOR CONCLUSIONSTEM techniques are powerful tools for the investigation of EVs and have the potential to reveal sample purity, ultrastructure and molecular composition. However, technical challenges, procedural errors in sample processing or misinterpretations may result in a variety of different morphologies and artifacts.The last decades have seen exponential technological progress in EV imaging by TEM. Nevertheless, protocols have not been standardized yet and sample preparation remains a critical step. An optimized, standardized and integrated protocol of different techniques could minimize artifacts and interpretative errors that could significantly improve the quality and reliability of downstream studies.GENERAL SIGNIFICANCEThe last decades have seen exponential technological progress in EV imaging by TEM. Nevertheless, protocols have not been standardized yet and sample preparation remains a critical step. An optimized, standardized and integrated protocol of different techniques could minimize artifacts and interpretative errors that could significantly improve the quality and reliability of downstream studies. Extracellular vesicles (EVs) are cell-derived nanometric particles governing the complex interactions among cells through their bioactive cargo. Interest in EVs is rapidly increasing due to their extensive involvement in physiological and pathological conditions, their potential employment as diagnostic and therapeutic tools and their prospective use as bio-carriers of exogenous molecules. Given their nanometric size, transmission electron microscopy (TEM) provides significant contributions to assess EV presence and purity in a sample and to study morphological features.In this review, TEM methods for EV imaging are compared with respect to their applications, benefits and drawbacks. A critical evaluation of the actual contribution of TEM to the study of EVs is also provided and the most common artifacts encountered in the literature are discussed.TEM techniques are powerful tools for the investigation of EVs and have the potential to reveal sample purity, ultrastructure and molecular composition. However, technical challenges, procedural errors in sample processing or misinterpretations may result in a variety of different morphologies and artifacts.The last decades have seen exponential technological progress in EV imaging by TEM. Nevertheless, protocols have not been standardized yet and sample preparation remains a critical step. An optimized, standardized and integrated protocol of different techniques could minimize artifacts and interpretative errors that could significantly improve the quality and reliability of downstream studies. |
| ArticleNumber | 129648 |
| Author | Pascucci, L. Scattini, G. |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32485221$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1038/srep14664 10.1146/annurev-cellbio-101512-122326 10.1002/cpsc.55 10.1073/pnas.1521230113 10.3402/jev.v3.24215 10.1242/dmm.028779 10.1007/s11095-014-1593-y 10.1016/j.aanat.2018.02.004 10.3402/jev.v5.31427 10.3934/biophy.2015.3.245 10.9729/AM.2017.47.3.171 10.1083/jcb.57.2.551 10.1038/sj.emboj.7600366 10.2174/1876822901003010001 10.1007/s10354-018-0646-y 10.1248/bpb.34.13 10.1016/j.nano.2015.01.003 10.1074/jbc.REV118.005602 10.4319/mave.2010.978-0-9845591-0-7.182 10.1016/j.yexcr.2011.01.014 10.1007/s00216-015-8535-3 10.3389/fphys.2019.00226 10.1016/j.jri.2008.06.001 10.1146/annurev.biochem.73.011303.074112 10.1111/jcmm.12436 10.1080/20013078.2019.1648996 10.1186/s12929-018-0494-5 10.1039/C9LC00526A 10.1038/nprot.2007.452 10.1039/C7BM00479F 10.4172/jpb.10000e10 10.1080/20013078.2019.1565263 10.1097/MBC.0b013e32831be9c5 10.1161/CIRCRESAHA.110.226456 10.3402/jev.v2i0.20389 10.3390/bioengineering6010007 10.1016/j.jconrel.2014.07.042 10.1007/978-1-4939-7253-1_18 10.1002/adma.201501015 10.1083/jcb.201304193 10.1002/1521-4141(2001010)31:10<2892::AID-IMMU2892>3.0.CO;2-I 10.1002/0471143030.cb0322s30 10.1016/j.transci.2017.03.007 10.1155/2016/7932765 10.1007/s00418-008-0500-1 10.1016/j.yexcr.2019.02.004 10.1038/labinvest.2010.152 10.3402/jev.v3.26913 10.1007/BF00337312 10.1080/20013078.2018.1555419 10.1096/fj.11-202077 10.1161/CIRCRESAHA.117.309417 10.1371/journal.pone.0083680 10.3402/jev.v4.28680 10.1016/S0959-8049(02)00596-8 10.1155/2019/4957806 10.1038/s12276-019-0223-5 10.1093/intimm/dxh267 10.1186/s13287-019-1432-5 10.1073/pnas.0403453101 10.1242/jcs.02439 10.1002/advs.201600476 10.1083/jcb.41.1.59 10.1080/09537104.2016.1268255 10.4049/jimmunol.179.3.1969 10.1080/20013078.2018.1535750 10.1016/j.semcdb.2015.02.007 10.1007/s00018-018-2773-4 10.1083/jcb.201211138 10.1016/j.colsurfb.2011.05.013 10.1111/jcmm.13479 |
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| References | Franken, Boekema, Stuart (bb0285) 2017; 4 Almizraq, Seghatchian, Holovati, Acker (bb0425) 2017; 56 Brisson, Tan, Linares, Gounou, Arraud (bb0250) 2017; 28 Koning, Koster, Sharp (bb0190) 2018; 217 Jeong, Shin, Yi, Park, Lee, Gianchandani, Park (bb0420) 2019; 19 Anderson (bb0130) 1969; 41 Caby, Lankar, Vincendeau-Scherrer, Raposo, Bonnerot (bb0060) 2005; 17 Linares, Tan, Gounou, Brisson (bb0320) 2014; 1545 Coumans, Brisson, Buzas, Dignat-George, Drees, El-Andaloussi, Emanueli, Gasecka, Hendrix, Hill, Lacroix, Lee, Van Leeuwen, Mackman, Mäger, Nolan, Van Der Pol, Pegtel, Sahoo, Siljander, Sturk, De Wever, Nieuwland (bb0160) 2017; 120 Al-Amoudi, Chang, Leforestier, McDowall, Salamin, Norlen, Richter, Blanc, Studer, Dubochet (bb0335) 2004; 23 Lötvall, Hill, Hochberg, Buzás, Di Vizio, Gardiner, Gho, Kurochkin, Mathivanan, Quesenberry, Sahoo, Tahara, Wauben, Witwer, Théry (bb0155) 2014; 3 Gallart-Palau, Serra, Wong, Sandin, Lai, Chen, Kon, Sze (bb0350) 2015; 5 Zonneveld, Brisson, van Herwijnen, Tan, van de Lest, Redegeld, Garssen, Wauben, Nolte-'t Hoe (bb0430) 2014; 3 Beit-Yannai, Tabak, Stamer (bb0245) 2018; 22 Gould, Raposo (bb0150) 2013; 2 Igami, Uchiumi, Ueda, Kamioka, Setoyama, Gotoh, Akimoto, Matsumoto, Kang (bb0295) 2019 Murphy, de Jong, Brouwer, Wood, Lavieu, Schiffelers, Vader (bb0030) 2019; 51 Meehan, Rak, Di Vizio (bb0125) 2016; 1 Lyumkis (bb0185) 2019; 294 Cizmar, Yuana (bb0220) 2017; 1660 Visnovitz, Osteikoetxea, Sódar, Mihály, Lőrincz, Vukman, Tóth, Koncz, Székács, Horváth, Varga, Buzás (bb0305) 2019; 8 Milasan, Tessandier, Tan, Brisson, Boilard, Martel (bb0385) 2016; 5 Ronquist, Brody (bb0070) 1985; 822 Scarff, Fuller, Thompson, Iadaza (bb0270) 2018; 2018 Fertig, Gherghiceanu, Popescu (bb0095) 2014; 18 Tomography, Plitzko, Baumeister (bb0330) 2019 Admyre, Johansson, Qazi, Filén, Lahesmaa, Norman, Neve, Scheynius, Gabrielsson (bb0080) 2007; 179 Yoshimura, Adachi, Matsuno, Kawamata, Yoshioka, Kikuchi, Odaka, Numakawa, Kunugi, Ochiya, Tamai (bb0260) 2018; 11 Lučić, Förster, Baumeister (bb0370) 2005; 74 Studer, Humbel, Chiquet (bb0195) 2008; 130 Kim, Song, Park, Kang, Lee, Lee, Kim, Ryu, Bae, Kim (bb0410) 2003; 39 Chuo, Chien, Lai (bb0340) 2018; 25 Tokuyasu (bb0390) 1973; 57 Théry, Witwer, Aikawa, Alcaraz, Anderson, Andriantsitohaina, Antoniou, Arab, Archer, Atkin-Smith, Ayre, Bach, Bachurski, Baharvand, Balaj, Baldacchino, Bauer, Baxter, Bebawy, Beckham, Zavec, Benmoussa, Berardi, Bergese, Bielska, Blenkiron, Bobis-Wozowicz, Boilard, Boireau, Bongiovanni, Borràs, Bosch, Boulanger, Breakefield, Breglio, Brennan, Brigstock, Brisson, Broekman, Bromberg, Bryl-Górecka, Buch, Buck, Burger, Busatto, Buschmann, Bussolati, Buzás, Byrd, Camussi, Carter, Caruso, Chamley, Chang, Chaudhuri, Chen, Chen, Cheng, Chin, Clayton, Clerici, Cocks, Cocucci, Coffey, Cordeiro-da-Silva, Couch, Coumans, Coyle, Crescitelli, Criado, D’Souza-Schorey, Das, de Candia, De Santana, De Wever, del Portillo, Demaret, Deville, Devitt, Dhondt, Di Vizio, Dieterich, Dolo, Rubio, Dominici, Dourado, Driedonks, Duarte, Duncan, Eichenberger, Ekström, Andaloussi, Elie-Caille, Erdbrügger, Falcón-Pérez, Fatima, Fish, Flores-Bellver, Försönits, Frelet-Barrand, Fricke, Fuhrmann, Gabrielsson, Gámez-Valero, Gardiner, Gärtner, Gaudin, Gho, Giebel, Gilbert, Gimona, Giusti, Goberdhan, Görgens, Gorski, Greening, Gross, Gualerzi, Gupta, Gustafson, Handberg, Haraszti, Harrison, Hegyesi, Hendrix, Hill, Hochberg, Hoffmann, Holder, Holthofer, Hosseinkhani, Hu, Huang, Huber, Hunt, Ibrahim, Ikezu, Inal, Isin, Ivanova, Jackson, Jacobsen, Jay, Jayachandran, Jenster, Jiang, Johnson, Jones, Jong, Jovanovic-Talisman, Jung, Kalluri, Kano, Kaur, Kawamura, Keller, Khamari, Khomyakova, Khvorova, Kierulf, Kim, Kislinger, Klingeborn, Klinke, Kornek, Kosanović, Kovács, Krämer-Albers, Krasemann, Krause, Kurochkin, Kusuma, Kuypers, Laitinen, Langevin, Languino, Lannigan, Lässer, Laurent, Lavieu, Lázaro-Ibáñez, Le Lay, Lee, Lee, Lemos, Lenassi, Leszczynska, Li, Liao, Libregts, Ligeti, Lim, Lim, Linē, Linnemannstöns, Llorente, Lombard, Lorenowicz, Lörincz, Lötvall, Lovett, Lowry, Loyer, Lu, Lukomska, Lunavat, Maas, Malhi, Marcilla, Mariani, Mariscal, Martens-Uzunova, Martin-Jaular, Martinez, Martins, Mathieu, Mathivanan, Maugeri, McGinnis, McVey, Meckes, Meehan, Mertens, Minciacchi, Möller, Jørgensen, Morales-Kastresana, Morhayim, Mullier, Muraca, Musante, Mussack, Muth, Myburgh, Najrana, Nawaz, Nazarenko, Nejsum, Neri, Neri, Nieuwland, Nimrichter, Nolan, Nolte-’t Hoen, Hooten, O’Driscoll, O’Grady, O’Loghlen, Ochiya, Olivier, Ortiz, Ortiz, Osteikoetxea, Ostegaard, Ostrowski, Park, Pegtel, Peinado, Perut, Pfaffl, Phinney, Pieters, Pink, Pisetsky, von Strandmann, Polakovicova, Poon, Powell, Prada, Pulliam, Quesenberry, Radeghieri, Raffai, Raimondo, Rak, Ramirez, Raposo, Rayyan, Regev-Rudzki, Ricklefs, Robbins, Roberts, Rodrigues, Rohde, Rome, Rouschop, Rughetti, Russell, Saá, Sahoo, Salas-Huenuleo, Sánchez, Saugstad, Saul, Schiffelers, Schneider, Schøyen, Scott, Shahaj, Sharma, Shatnyeva, Shekari, Shelke, Shetty, Shiba, Siljander, Silva, Skowronek, Snyder, Soares, Sódar, Soekmadji, Sotillo, Stahl, Stoorvogel, Stott, Strasser, Swift, Tahara, Tewari, Timms, Tiwari, Tixeira, Tkach, Toh, Tomasini, Torrecilhas, Tosar, Toxavidis, Urbanelli, Vader, van Balkom, van der Grein, Van Deun, van Herwijnen, Van Keuren-Jensen, van Niel, van Royen, van Wijnen, Vasconcelos, Vechetti, Veit, Vella, Velot, Verweij, Vestad, Viñas, Visnovitz, Vukman, Wahlgren, Watson, Wauben, Weaver, Webber, Weber, Wehman, Weiss, Welsh, Wendt, Wheelock, Wiener, Witte, Wolfram, Xagorari, Xander, Xu, Yan, Yáñez-Mó, Yin, Yuana, Zappulli, Zarubova, Žėkas, de Zhang, Zhao, Zheng, Zheutlin, Zickler, Zimmermann, Zivkovic, Zocco, Zuba-Surma (bb0025) 2018; 7 Coleman, Hill (bb0345) 2015; 40 Sokolova, Ludwig, Hornung, Rotan, Horn, Epple, Giebel (bb0180) 2011; 87 Grassucci, Taylor, Frank (bb0440) 2007; 2 Lawson, Kovacs, Finding, Ulfelder, Luis-Fuentes (bb0005) 2017; 90 Wolf (bb0010) 1967 Wang, Lee (bb0170) 2015 Arasu, Härkönen, Koistinen, Rilla (bb0265) 2019; 376 Neumüller (bb0360) 2018; 168 Coleman, Hanssen, Lawson, Hill (bb0240) 2012; 26 Issman, Brenner, Talmon, Aharon (bb0235) 2013; 8 Asea, Jean-Pierre, Kaur, Rao, Linhares, Skupski, Witkin (bb0085) 2008; 79 Babiker, Ronquist, Nilsson, Ekdahl (bb0110) 2010; 3 Théry, Clayton, Amigorena, Raposo (bb0205) 2006; 30 Atay, Gercel-Taylor, Kesimer, Taylor (bb0310) 2011; 317 Peter Ercius, Alaidi, Rames (bb0355) 2015; 27 Bonucci (bb0135) 1970; 103 Bodart-Santos, de Carvalho, de Godoy, Batista, Saraiva, Lima, Abreu, De Felice, Galina, Mendez-Otero, Ferreira (bb0255) 2019; 10 Raposo, Stoorvogel (bb0055) 2013; 200 Brisson (bb0445) 2019; 8 Chernyshev, Rachamadugu, Tseng, Belnap, Jia, Branch, Butterfield, Pease, Bernard, Skliar (bb0225) 2015; 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11 Ogawa, Miura, Harazono, Kanai-Azuma, Akimoto, Kawakami, Yamaguchi, Toda, Endo, Tsubuki, Yanoshita (bb0075) 2011; 34 Tang, Lv, Lan, Liu (bb0045) 2019; 10 Pisitkun, Shen, Knepper (bb0065) 2004; 101 Jia, Guan, Zheng, Zhang, Tang, Xu, Xiao, Wang, Xue (bb0300) 2016; 2016 Jung, Mun (bb0290) 2018; 2018 Lučić, Rigort, Baumeister (bb0365) 2013; 202 Capomaccio, Cappelli, Bazzucchi, Coletti, Gialletti, Moriconi, Passamonti, Pepe, Petrini, Mecocci, Silvestrelli, Pascucci (bb0380) 2019; 2019 Karlsson, Lundin, Dahlgren, Kahu, Pettersson, Telemo (bb0120) 2001; 31 Pascucci, Coccè, Bonomi, Ami, Ceccarelli, Ciusani, Viganò, Locatelli, Sisto, Doglia, Parati, Bernardo, Muraca, Alessandri, Bondiolotti, Pessina (bb0050) 2014; 192 Monnier, Thévenaz, Balog, Fiore, Vanhecke, Rothen-Rutishauser, Petri-Fink (bb0415) 2015; 2 Yang, Martin, Fogarty, Brown, Schurman, Phipps, Yin, Lockman, Bai (bb0040) 2015; 32 Karimi, Cvjetkovic, Jang, Crescitelli, Hosseinpour Feizi, Nieuwland, Lötvall, Lässer (bb0395) 2018; 75 Hartjes, Mytnyk, Jenster, van Steijn, van Royen (bb0200) 2019; 6 Capomaccio, Cappelli, Bazzucchi, Coletti, Gialletti, Moriconi, Passamonti, Pepe, Petrini, Mecocci, Silvestrelli, Pascucci (bb0230) 2019; 2019 Choi, Mun (bb0315) 2017; 47 Lo Sicco, Reverberi, Pascucci, Tasso (bb0210) 2018; 46 Wang, Lee (bb0175) 2015 Rikkert, Nieuwland, Terstappen, Coumans (bb0215) 2019; 8 Issman (10.1016/j.bbagen.2020.129648_bb0235) 2013; 8 Al-Amoudi (10.1016/j.bbagen.2020.129648_bb0335) 2004; 23 Simpson (10.1016/j.bbagen.2020.129648_bb0140) 2012; 5 Grassucci (10.1016/j.bbagen.2020.129648_bb0440) 2007; 2 Tang (10.1016/j.bbagen.2020.129648_bb0045) 2019; 10 Kowal (10.1016/j.bbagen.2020.129648_bb0145) 2016; 113 Meehan (10.1016/j.bbagen.2020.129648_bb0125) 2016; 1 Beit-Yannai (10.1016/j.bbagen.2020.129648_bb0245) 2018; 22 Murphy (10.1016/j.bbagen.2020.129648_bb0030) 2019; 51 Wolf (10.1016/j.bbagen.2020.129648_bb0010) 1967 Linares (10.1016/j.bbagen.2020.129648_bb0320) 2014; 1545 Ogawa (10.1016/j.bbagen.2020.129648_bb0075) 2011; 34 Lötvall (10.1016/j.bbagen.2020.129648_bb0155) 2014; 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90 Höög (10.1016/j.bbagen.2020.129648_bb0375) 2015; 4 Mause (10.1016/j.bbagen.2020.129648_bb0100) 2010; 107 Scarff (10.1016/j.bbagen.2020.129648_bb0270) 2018; 2018 Mielańczyk (10.1016/j.bbagen.2020.129648_bb0165) 2015 Karlsson (10.1016/j.bbagen.2020.129648_bb0120) 2001; 31 Wang (10.1016/j.bbagen.2020.129648_bb0175) 2015 Jia (10.1016/j.bbagen.2020.129648_bb0300) 2016; 2016 Neumüller (10.1016/j.bbagen.2020.129648_bb0360) 2018; 168 Coleman (10.1016/j.bbagen.2020.129648_bb0345) 2015; 40 Rikkert (10.1016/j.bbagen.2020.129648_bb0215) 2019; 8 Mincheva-Nilsson (10.1016/j.bbagen.2020.129648_bb0275) 2016; 2016 Yang (10.1016/j.bbagen.2020.129648_bb0040) 2015; 32 Ackermann (10.1016/j.bbagen.2020.129648_bb0280) 2010 Pisitkun (10.1016/j.bbagen.2020.129648_bb0065) 2004; 101 Visnovitz (10.1016/j.bbagen.2020.129648_bb0305) 2019; 8 Théry (10.1016/j.bbagen.2020.129648_bb0205) 2006; 30 Capomaccio (10.1016/j.bbagen.2020.129648_bb0230) 2019; 2019 Brisson (10.1016/j.bbagen.2020.129648_bb0250) 2017; 28 Jeong (10.1016/j.bbagen.2020.129648_bb0420) 2019; 19 Asea (10.1016/j.bbagen.2020.129648_bb0085) 2008; 79 Gould (10.1016/j.bbagen.2020.129648_bb0150) 2013; 2 Raposo (10.1016/j.bbagen.2020.129648_bb0055) 2013; 200 Lyumkis (10.1016/j.bbagen.2020.129648_bb0185) 2019; 294 Colombo (10.1016/j.bbagen.2020.129648_bb0020) 2014; 30 Tomography (10.1016/j.bbagen.2020.129648_bb0330) 2019 Ronquist (10.1016/j.bbagen.2020.129648_bb0070) 1985; 822 Koning (10.1016/j.bbagen.2020.129648_bb0190) 2018; 217 Admyre (10.1016/j.bbagen.2020.129648_bb0080) 2007; 179 Fertig (10.1016/j.bbagen.2020.129648_bb0095) 2014; 18 Michen (10.1016/j.bbagen.2020.129648_bb0325) 2014; 3 Choi (10.1016/j.bbagen.2020.129648_bb0315) 2017; 47 Zonneveld (10.1016/j.bbagen.2020.129648_bb0430) 2014; 3 Studer (10.1016/j.bbagen.2020.129648_bb0195) 2008; 130 Gallart-Palau (10.1016/j.bbagen.2020.129648_bb0350) 2015; 5 Capomaccio (10.1016/j.bbagen.2020.129648_bb0380) 2019; 2019 Lo Sicco (10.1016/j.bbagen.2020.129648_bb0210) 2018; 46 Bodart-Santos (10.1016/j.bbagen.2020.129648_bb0255) 2019; 10 Anderson (10.1016/j.bbagen.2020.129648_bb0015) 2010; 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| References_xml | – volume: 2018 start-page: 5 year: 2018 end-page: 9 ident: bb0290 article-title: Sample preparation and imaging of exosomes by transmission electron microscopy publication-title: J. Vis. Exp. – volume: 179 start-page: 1969 year: 2007 end-page: 1978 ident: bb0080 article-title: Exosomes with immune modulatory features are present in human breast milk publication-title: J. Immunol. – volume: 8 start-page: 8 year: 2019 end-page: 9 ident: bb0445 article-title: Comment on “quality of extracellular vesicle images by transmission electron microscopy is operator and protocol dependent” publication-title: J. Extracell. Vesicles – volume: 120 start-page: 1632 year: 2017 end-page: 1648 ident: bb0160 article-title: Methodological guidelines to study extracellular vesicles publication-title: Circ. Res. – volume: 25 year: 2018 ident: bb0340 article-title: Imaging extracellular vesicles: current and emerging methods publication-title: J. Biomed. Sci. – volume: 90 start-page: 481 year: 2017 end-page: 491 ident: bb0005 article-title: Extracellular vesicles: evolutionarily conserved mediators of intercellular communication publication-title: Yale J. Biol. Med. – volume: 317 start-page: 1192 year: 2011 end-page: 1202 ident: bb0310 article-title: Morphologic and proteomic characterization of exosomes released by cultured extravillous trophoblast cells publication-title: Exp. Cell Res. – volume: 101 start-page: 13368 year: 2004 end-page: 13373 ident: bb0065 article-title: Identification and proteomic profiling of exosomes in human urine publication-title: Proc. Natl. Acad. Sci. U. S. A. – volume: 3 start-page: 1 year: 2014 end-page: 6 ident: bb0155 article-title: Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles publication-title: J. Extracell. Vesicles. – year: 2015 ident: bb0175 article-title: Electron Microscopy Techniques for Imaging and Analysis of Nanoparticles – volume: 10 start-page: 332 year: 2019 ident: bb0255 article-title: Extracellular vesicles derived from human Wharton’s jelly mesenchymal stem cells protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-β oligomers publication-title: Stem Cell Res Ther – volume: 18 start-page: 1938 year: 2014 end-page: 1943 ident: bb0095 article-title: Extracellular vesicles release by cardiac telocytes: electron microscopy and electron tomography publication-title: J. Cell. Mol. Med. – volume: 10 start-page: 111 year: 2017 end-page: 130 ident: bb0400 article-title: Purification protocols for extracellular vesicles publication-title: Extracell. Vesicles – volume: 20 start-page: 52 year: 2009 end-page: 56 ident: bb0435 article-title: Recovery and composition of microparticles after snap-freezing depends on thawing temperature publication-title: Blood Coagul. Fibrinolysis – volume: 27 start-page: 5638 year: 2015 end-page: 5663 ident: bb0355 article-title: Electron tomography: a three-dimensional analytic tool for hard and soft materials research publication-title: Adv Mater. – volume: 30 start-page: 255 year: 2014 end-page: 289 ident: bb0020 article-title: Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles publication-title: Annu. Rev. Cell Dev. Biol. – volume: 2016 year: 2016 ident: bb0300 article-title: MiRNAs in urine extracellular vesicles as predictors of early-stage diabetic nephropathy publication-title: J. Diabetes Res. – volume: 168 start-page: 322 year: 2018 end-page: 329 ident: bb0360 article-title: Electron tomography—a tool for ultrastructural 3D visualization in cell biology and histology publication-title: Wien. Med. Wochenschr. – volume: 11 start-page: 879 year: 2015 end-page: 883 ident: bb0405 article-title: Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties publication-title: Nanomed. Nanotechnol. Biol. Med. – volume: 2 year: 2013 ident: bb0150 article-title: As we wait: coping with an imperfect nomenclature for extracellular vesicles publication-title: J. Extracell. Vesicl. – year: 1967 ident: bb0010 article-title: The Nature and Significance of Platelet Products in Human Plasma – start-page: 396 year: 2015 end-page: 405 ident: bb0035 article-title: Delivery – volume: 4 year: 2017 ident: bb0285 article-title: Transmission electron microscopy as a tool for the characterization of soft materials: application and interpretation publication-title: Adv. Sci. – volume: 9 year: 2020 ident: bb0090 article-title: Apoptotic bodies: Particular extracellular vesicles involved in intercellular communication publication-title: Biology (Basel) – volume: 75 start-page: 2873 year: 2018 end-page: 2886 ident: bb0395 article-title: Detailed analysis of the plasma extracellular vesicle proteome after separation from lipoproteins publication-title: Cell. Mol. Life Sci. – volume: 31 start-page: 2892 year: 2001 end-page: 2900 ident: bb0120 article-title: ‘Tolerosomes’ are produced by intestinal epithelial cells publication-title: Eur. J. Immunol. – volume: 1 start-page: 4 year: 2016 end-page: 5 ident: bb0125 article-title: Oncosomes - large and small what are they, where they came from? publication-title: J. Extracell. Vesicl. – volume: 217 start-page: 82 year: 2018 end-page: 96 ident: bb0190 article-title: Advances in cryo-electron tomography for biology and medicine publication-title: Ann. Anat. – volume: 8 year: 2019 ident: bb0305 article-title: An improved 96 well plate format lipid quantification assay for standardisation of experiments with extracellular vesicles publication-title: J. Extracell. Vesicles – volume: 294 start-page: 5181 year: 2019 end-page: 5197 ident: bb0185 article-title: Challenges and opportunities in cryo-EM single-particle analysis publication-title: J. Biol. Chem. – volume: 74 start-page: 833 year: 2005 end-page: 865 ident: bb0370 article-title: Structural studies by electron tomography: from cells to molecules publication-title: Annu. Rev. Biochem. – volume: 200 start-page: 373 year: 2013 end-page: 383 ident: bb0055 article-title: Extracellular vesicles: Exosomes, microvesicles, and friends publication-title: J. Cell Biol. – volume: 30 start-page: 1 year: 2006 end-page: 29 ident: bb0205 article-title: Isolation and characterization of exosomes from cell culture supernatants publication-title: Curr. Protoc. Cell Biol. – volume: 2018 start-page: 1 year: 2018 end-page: 8 ident: bb0270 article-title: Variations on negative stain electron microscopy methods: tools for tackling challenging systems publication-title: J. Vis. Exp. – volume: 87 start-page: 146 year: 2011 end-page: 150 ident: bb0180 article-title: Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy publication-title: Colloid. Surf. B Biointerf. – volume: 46 year: 2018 ident: bb0210 article-title: A method for isolating and characterizing mesenchymal stromal cell-derived extracellular vesicles publication-title: Curr. Protoc. Stem Cell Biol. – volume: 1545 start-page: 139 year: 2014 end-page: 176 ident: bb0320 article-title: Exosomes and Microvesicles: Methods and Protocols – volume: 2 start-page: 3239 year: 2007 end-page: 3246 ident: bb0440 article-title: Preparation of macromolecular complexes for cryo-electron microscopy publication-title: Nat. Protoc. – volume: 2019 year: 2019 ident: bb0230 article-title: Equine adipose-derived mesenchymal stromal cells release extracellular vesicles enclosing different subsets of small RNAs publication-title: Stem Cells Int. – volume: 11 year: 2018 ident: bb0260 article-title: The Sox2 promoter-driven CD63-GFP transgenic rat model allows tracking of neural stem cell-derived extracellular vesicles publication-title: Dis. Model. Mech. – volume: 79 start-page: 12 year: 2008 end-page: 17 ident: bb0085 article-title: Heat shock protein-containing exosomes in mid-trimester amniotic fluids publication-title: J. Reprod. Immunol. – volume: 1660 start-page: 221 year: 2017 end-page: 232 ident: bb0220 article-title: Detection and characterization of extracellular vesicles by transmission and cryo-transmission electron microscopy publication-title: Methods Mol. Biol. – volume: 5 start-page: 1 year: 2015 end-page: 12 ident: bb0350 article-title: Extracellular vesicles are rapidly purified from human plasma by PRotein Organic Solvent PRecipitation (PROSPR) publication-title: Sci. Rep. – volume: 26 start-page: 4160 year: 2012 end-page: 4173 ident: bb0240 article-title: Prion-infected cells regulate the release of exosomes with distinct ultrastructural features publication-title: FASEB J. – volume: 51 year: 2019 ident: bb0030 article-title: Extracellular vesicle-based therapeutics: natural versus engineered targeting and trafficking publication-title: Exp. Mol. Med. – volume: 130 start-page: 877 year: 2008 end-page: 889 ident: bb0195 article-title: Electron microscopy of high pressure frozen samples: bridging the gap between cellular ultrastructure and atomic resolution publication-title: Histochem. Cell Biol. – volume: 8 year: 2013 ident: bb0235 article-title: Cryogenic transmission electron microscopy nanostructural study of shed microparticles publication-title: PLoS One – volume: 28 start-page: 263 year: 2017 end-page: 271 ident: bb0250 article-title: Extracellular vesicles from activated platelets: a semiquantitative cryo-electron microscopy and immuno-gold labeling study publication-title: Platelets – volume: 40 start-page: 89 year: 2015 end-page: 96 ident: bb0345 article-title: Extracellular vesicles - their role in the packaging and spread of misfolded proteins associated with neurodegenerative diseases publication-title: Semin. Cell Dev. Biol. – volume: 822 start-page: 203 year: 1985 end-page: 218 ident: bb0070 article-title: The prostasome: its secretion and function in man publication-title: Rev. Biomembr. – start-page: 623553 year: 2019 ident: bb0295 article-title: Characterization and function of medium and large extracellular vesicles from plasma and urine by surface antigens and Annexin V publication-title: BioRxiv – volume: 17 start-page: 879 year: 2005 end-page: 887 ident: bb0060 article-title: Exosomal-like vesicles are present in human blood plasma publication-title: Int. Immunol. – volume: 3 start-page: 39 year: 2014 end-page: 41 ident: bb0325 article-title: TEM sample preparation of nanoparticles in suspensions publication-title: Imag. Microsc. – volume: 2019 year: 2019 ident: bb0380 article-title: Equine adipose-derived mesenchymal stromal cells release extracellular vesicles enclosing different subsets of small RNAs publication-title: Stem Cells Int. – volume: 32 start-page: 2003 year: 2015 end-page: 2014 ident: bb0040 article-title: Exosome delivered anticancer drugs across the blood-brain barrier for brain cancer therapy in Danio Rerio publication-title: Pharm. Res. – volume: 39 start-page: 184 year: 2003 end-page: 191 ident: bb0410 article-title: Elevated levels of circulating platelet microparticles, VEGF, IL-6 and RANTES in patients with gastric cancer : possible role of a metastasis predictor publication-title: Eur. J. Cancer – volume: 4 start-page: 28680 year: 2015 ident: bb0375 article-title: Diversity of extracellular vesicles in human ejaculates revealed by cryo-electron microscopy publication-title: J. Extracell. Vesicles – volume: 103 start-page: 192 year: 1970 end-page: 217 ident: bb0135 article-title: Fine structure and histochemistry of ‘calcifying globules’ in epiphyseal cartilage publication-title: Zeitschrift Für Zellforsch. Und Mikroskopische Anat. – volume: 107 start-page: 1047 year: 2010 end-page: 1057 ident: bb0100 article-title: Microparticles protagonists of a novel communication network for intercellular information exchange publication-title: Circ. Res. – volume: 118 start-page: 2849 year: 2005 end-page: 2858 ident: bb0115 article-title: Release of extracellular membrane particles carrying the stem cell marker prominin-1 (CD133) from neural progenitors and other epithelial cells publication-title: J. Cell Sci. – volume: 22 start-page: 2001 year: 2018 end-page: 2006 ident: bb0245 article-title: Physical exosome:exosome interactions publication-title: J. Cell. Mol. Med. – volume: 3 start-page: 1 year: 2014 end-page: 12 ident: bb0430 article-title: Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures publication-title: J. Extracell. Vesicles – volume: 23 start-page: 3583 year: 2004 end-page: 3588 ident: bb0335 article-title: New EMBO member’s review cryo-electron microscopy of vitreous sections publication-title: EMBO J. – volume: 407 start-page: 3285 year: 2015 end-page: 3301 ident: bb0225 article-title: Size and shape characterization of hydrated and desiccated exosomes publication-title: Anal. Bioanal. Chem. – volume: 376 start-page: 149 year: 2019 end-page: 158 ident: bb0265 article-title: Correlative light and electron microscopy is a powerful tool to study interactions of extracellular vesicles with recipient cells publication-title: Exp. Cell Res. – volume: 56 start-page: 254 year: 2017 end-page: 260 ident: bb0425 article-title: Extracellular vesicle characteristics in stored red blood cell concentrates are influenced by the method of detection publication-title: Transfus. Apher. Sci. – volume: 7 year: 2018 ident: bb0025 article-title: Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines publication-title: J. Extracell. Vesicl. – volume: 6 start-page: 60 year: 2018 end-page: 78 ident: bb0105 article-title: Extracellular vesicles, exosomes and shedding vesicles in regenerative medicine-a new paradigm for tissue repair publication-title: Biomater. Sci. – volume: 41 start-page: 59 year: 1969 end-page: 72 ident: bb0130 article-title: Vesicles associated with calcification in the matrix of epiphyseal cartilage publication-title: J. Cell Biol. – volume: 57 start-page: 551 year: 1973 end-page: 565 ident: bb0390 article-title: A technique for ultracryotomy of cell suspensions and tissues publication-title: J. Cell Biol. – volume: 34 start-page: 13 year: 2011 end-page: 23 ident: bb0075 article-title: Proteomic analysis of two types of exosomes in human whole saliva publication-title: Biol. Pharm. Bull. – volume: 2 start-page: 245 year: 2015 end-page: 258 ident: bb0415 article-title: A guide to investigating colloidal nanoparticles by cryogenic transmission electron microscopy: pitfalls and benefits publication-title: AIMS Biophys. – start-page: 182 year: 2010 end-page: 192 ident: bb0280 article-title: Basic electron microscopy of aquatic viruses publication-title: Man. Aquat. Viral Ecol. – volume: 192 start-page: 262 year: 2014 end-page: 270 ident: bb0050 article-title: Paclitaxel is incorporated by mesenchymal stromal cells and released in exosomes that inhibit in vitro tumor growth: a new approach for drug delivery publication-title: J. Control. Release – volume: 5 start-page: 10000 year: 2012 ident: bb0140 article-title: Extracellular microvesicles: the need for internationally recognised nomenclature and stringent purification criteria publication-title: J. Proteom. Bioinform. – volume: 5 start-page: 1 year: 2016 end-page: 9 ident: bb0385 article-title: Extracellular vesicles are present in mouse lymph and their level differs in atherosclerosis publication-title: J. Extracell. Vesicles – volume: 3 start-page: 1 year: 2010 end-page: 13 ident: bb0110 article-title: Prostasome involvement in the development and growth of prostate cancer~!2009-07-10~!2009-12-09~!2010-02-11~! publication-title: Open Prost. Cancer J. – volume: 6 year: 2019 ident: bb0200 article-title: Extracellular vesicle quantification and characterization: common methods and emerging approaches publication-title: Bioengineering – volume: 47 start-page: 171 year: 2017 end-page: 175 ident: bb0315 article-title: Structural analysis of exosomes using different types of electron microscopy publication-title: Appl. Microsc. – volume: 90 start-page: 1549 year: 2010 end-page: 1557 ident: bb0015 article-title: Role of extracellular membrane vesicles in the pathogenesis of various diseases, including cancer, renal diseases, atherosclerosis, and arthritis publication-title: Lab. Investig. – volume: 113 start-page: E968 year: 2016 end-page: E977 ident: bb0145 article-title: Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes publication-title: Proc. Natl. Acad. Sci. U. S. A. – volume: 202 start-page: 407 year: 2013 end-page: 419 ident: bb0365 article-title: Cryo-electron tomography: the challenge of doing structural biology in situ publication-title: J. Cell Biol. – year: 2015 ident: bb0170 article-title: Electron Microscopy Techniques for Imaging and Analysis of Nanoparticles – volume: 8 year: 2019 ident: bb0215 article-title: Quality of extracellular vesicle images by transmission electron microscopy is operator and protocol dependent publication-title: J. Extracell. Vesicles – volume: 2016 start-page: 14.42.1 year: 2016 end-page: 14.42.21 ident: bb0275 article-title: Isolation and characterization of exosomes from cultures of tissue explants and cell lines publication-title: Curr. Protoc. Immunol. – year: 2015 ident: bb0165 article-title: Transmission electron microscopy of biological samples publication-title: Transm. Electron Microsc. Theory Appl. – volume: 19 start-page: 3326 year: 2019 end-page: 3336 ident: bb0420 article-title: Size-based analysis of extracellular vesicles using sequential transfer of an evaporating droplet publication-title: Lab Chip – volume: 10 start-page: 1 year: 2019 end-page: 12 ident: bb0045 article-title: Extracellular vesicles: opportunities and challenges for the treatment of renal diseases publication-title: Front. Physiol. – year: 2019 ident: bb0330 article-title: Cryo-electron – year: 2019 ident: 10.1016/j.bbagen.2020.129648_bb0330 – volume: 5 start-page: 1 year: 2015 ident: 10.1016/j.bbagen.2020.129648_bb0350 article-title: Extracellular vesicles are rapidly purified from human plasma by PRotein Organic Solvent PRecipitation (PROSPR) publication-title: Sci. Rep. doi: 10.1038/srep14664 – volume: 30 start-page: 255 year: 2014 ident: 10.1016/j.bbagen.2020.129648_bb0020 article-title: Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles publication-title: Annu. Rev. Cell Dev. Biol. doi: 10.1146/annurev-cellbio-101512-122326 – volume: 822 start-page: 203 year: 1985 ident: 10.1016/j.bbagen.2020.129648_bb0070 article-title: The prostasome: its secretion and function in man publication-title: Rev. Biomembr. – volume: 46 year: 2018 ident: 10.1016/j.bbagen.2020.129648_bb0210 article-title: A method for isolating and characterizing mesenchymal stromal cell-derived extracellular vesicles publication-title: Curr. Protoc. Stem Cell Biol. doi: 10.1002/cpsc.55 – volume: 113 start-page: E968 year: 2016 ident: 10.1016/j.bbagen.2020.129648_bb0145 article-title: Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.1521230113 – volume: 3 start-page: 1 year: 2014 ident: 10.1016/j.bbagen.2020.129648_bb0430 article-title: Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures publication-title: J. Extracell. Vesicles doi: 10.3402/jev.v3.24215 – volume: 11 year: 2018 ident: 10.1016/j.bbagen.2020.129648_bb0260 article-title: The Sox2 promoter-driven CD63-GFP transgenic rat model allows tracking of neural stem cell-derived extracellular vesicles publication-title: Dis. Model. Mech. doi: 10.1242/dmm.028779 – volume: 2018 start-page: 1 year: 2018 ident: 10.1016/j.bbagen.2020.129648_bb0270 article-title: Variations on negative stain electron microscopy methods: tools for tackling challenging systems publication-title: J. Vis. Exp. – volume: 32 start-page: 2003 year: 2015 ident: 10.1016/j.bbagen.2020.129648_bb0040 article-title: Exosome delivered anticancer drugs across the blood-brain barrier for brain cancer therapy in Danio Rerio publication-title: Pharm. Res. doi: 10.1007/s11095-014-1593-y – volume: 217 start-page: 82 year: 2018 ident: 10.1016/j.bbagen.2020.129648_bb0190 article-title: Advances in cryo-electron tomography for biology and medicine publication-title: Ann. Anat. doi: 10.1016/j.aanat.2018.02.004 – volume: 5 start-page: 1 year: 2016 ident: 10.1016/j.bbagen.2020.129648_bb0385 article-title: Extracellular vesicles are present in mouse lymph and their level differs in atherosclerosis publication-title: J. Extracell. Vesicles doi: 10.3402/jev.v5.31427 – volume: 2 start-page: 245 year: 2015 ident: 10.1016/j.bbagen.2020.129648_bb0415 article-title: A guide to investigating colloidal nanoparticles by cryogenic transmission electron microscopy: pitfalls and benefits publication-title: AIMS Biophys. doi: 10.3934/biophy.2015.3.245 – volume: 47 start-page: 171 year: 2017 ident: 10.1016/j.bbagen.2020.129648_bb0315 article-title: Structural analysis of exosomes using different types of electron microscopy publication-title: Appl. Microsc. doi: 10.9729/AM.2017.47.3.171 – start-page: 623553 year: 2019 ident: 10.1016/j.bbagen.2020.129648_bb0295 article-title: Characterization and function of medium and large extracellular vesicles from plasma and urine by surface antigens and Annexin V publication-title: BioRxiv – volume: 57 start-page: 551 year: 1973 ident: 10.1016/j.bbagen.2020.129648_bb0390 article-title: A technique for ultracryotomy of cell suspensions and tissues publication-title: J. Cell Biol. doi: 10.1083/jcb.57.2.551 – volume: 23 start-page: 3583 year: 2004 ident: 10.1016/j.bbagen.2020.129648_bb0335 article-title: New EMBO member’s review cryo-electron microscopy of vitreous sections publication-title: EMBO J. doi: 10.1038/sj.emboj.7600366 – volume: 3 start-page: 1 year: 2010 ident: 10.1016/j.bbagen.2020.129648_bb0110 article-title: Prostasome involvement in the development and growth of prostate cancer~!2009-07-10~!2009-12-09~!2010-02-11~! publication-title: Open Prost. Cancer J. doi: 10.2174/1876822901003010001 – volume: 168 start-page: 322 year: 2018 ident: 10.1016/j.bbagen.2020.129648_bb0360 article-title: Electron tomography—a tool for ultrastructural 3D visualization in cell biology and histology publication-title: Wien. Med. Wochenschr. doi: 10.1007/s10354-018-0646-y – volume: 34 start-page: 13 year: 2011 ident: 10.1016/j.bbagen.2020.129648_bb0075 article-title: Proteomic analysis of two types of exosomes in human whole saliva publication-title: Biol. Pharm. Bull. doi: 10.1248/bpb.34.13 – volume: 11 start-page: 879 year: 2015 ident: 10.1016/j.bbagen.2020.129648_bb0405 article-title: Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties publication-title: Nanomed. Nanotechnol. Biol. Med. doi: 10.1016/j.nano.2015.01.003 – volume: 294 start-page: 5181 year: 2019 ident: 10.1016/j.bbagen.2020.129648_bb0185 article-title: Challenges and opportunities in cryo-EM single-particle analysis publication-title: J. Biol. Chem. doi: 10.1074/jbc.REV118.005602 – start-page: 182 year: 2010 ident: 10.1016/j.bbagen.2020.129648_bb0280 article-title: Basic electron microscopy of aquatic viruses publication-title: Man. Aquat. Viral Ecol. doi: 10.4319/mave.2010.978-0-9845591-0-7.182 – volume: 2016 start-page: 14.42.1 year: 2016 ident: 10.1016/j.bbagen.2020.129648_bb0275 article-title: Isolation and characterization of exosomes from cultures of tissue explants and cell lines publication-title: Curr. Protoc. Immunol. – volume: 317 start-page: 1192 year: 2011 ident: 10.1016/j.bbagen.2020.129648_bb0310 article-title: Morphologic and proteomic characterization of exosomes released by cultured extravillous trophoblast cells publication-title: Exp. Cell Res. doi: 10.1016/j.yexcr.2011.01.014 – volume: 407 start-page: 3285 year: 2015 ident: 10.1016/j.bbagen.2020.129648_bb0225 article-title: Size and shape characterization of hydrated and desiccated exosomes publication-title: Anal. Bioanal. Chem. doi: 10.1007/s00216-015-8535-3 – start-page: 396 year: 2015 ident: 10.1016/j.bbagen.2020.129648_bb0035 – volume: 10 start-page: 1 year: 2019 ident: 10.1016/j.bbagen.2020.129648_bb0045 article-title: Extracellular vesicles: opportunities and challenges for the treatment of renal diseases publication-title: Front. Physiol. doi: 10.3389/fphys.2019.00226 – volume: 79 start-page: 12 year: 2008 ident: 10.1016/j.bbagen.2020.129648_bb0085 article-title: Heat shock protein-containing exosomes in mid-trimester amniotic fluids publication-title: J. Reprod. Immunol. doi: 10.1016/j.jri.2008.06.001 – volume: 74 start-page: 833 year: 2005 ident: 10.1016/j.bbagen.2020.129648_bb0370 article-title: Structural studies by electron tomography: from cells to molecules publication-title: Annu. Rev. Biochem. doi: 10.1146/annurev.biochem.73.011303.074112 – volume: 18 start-page: 1938 year: 2014 ident: 10.1016/j.bbagen.2020.129648_bb0095 article-title: Extracellular vesicles release by cardiac telocytes: electron microscopy and electron tomography publication-title: J. Cell. Mol. Med. doi: 10.1111/jcmm.12436 – volume: 8 start-page: 8 year: 2019 ident: 10.1016/j.bbagen.2020.129648_bb0445 article-title: Comment on “quality of extracellular vesicle images by transmission electron microscopy is operator and protocol dependent” publication-title: J. Extracell. Vesicles doi: 10.1080/20013078.2019.1648996 – volume: 25 year: 2018 ident: 10.1016/j.bbagen.2020.129648_bb0340 article-title: Imaging extracellular vesicles: current and emerging methods publication-title: J. Biomed. Sci. doi: 10.1186/s12929-018-0494-5 – volume: 19 start-page: 3326 year: 2019 ident: 10.1016/j.bbagen.2020.129648_bb0420 article-title: Size-based analysis of extracellular vesicles using sequential transfer of an evaporating droplet publication-title: Lab Chip doi: 10.1039/C9LC00526A – volume: 3 start-page: 39 year: 2014 ident: 10.1016/j.bbagen.2020.129648_bb0325 article-title: TEM sample preparation of nanoparticles in suspensions publication-title: Imag. Microsc. – volume: 2 start-page: 3239 year: 2007 ident: 10.1016/j.bbagen.2020.129648_bb0440 article-title: Preparation of macromolecular complexes for cryo-electron microscopy publication-title: Nat. Protoc. doi: 10.1038/nprot.2007.452 – volume: 6 start-page: 60 year: 2018 ident: 10.1016/j.bbagen.2020.129648_bb0105 article-title: Extracellular vesicles, exosomes and shedding vesicles in regenerative medicine-a new paradigm for tissue repair publication-title: Biomater. Sci. doi: 10.1039/C7BM00479F – volume: 5 start-page: 10000 year: 2012 ident: 10.1016/j.bbagen.2020.129648_bb0140 article-title: Extracellular microvesicles: the need for internationally recognised nomenclature and stringent purification criteria publication-title: J. Proteom. Bioinform. doi: 10.4172/jpb.10000e10 – volume: 8 year: 2019 ident: 10.1016/j.bbagen.2020.129648_bb0305 article-title: An improved 96 well plate format lipid quantification assay for standardisation of experiments with extracellular vesicles publication-title: J. Extracell. Vesicles doi: 10.1080/20013078.2019.1565263 – volume: 20 start-page: 52 year: 2009 ident: 10.1016/j.bbagen.2020.129648_bb0435 article-title: Recovery and composition of microparticles after snap-freezing depends on thawing temperature publication-title: Blood Coagul. Fibrinolysis doi: 10.1097/MBC.0b013e32831be9c5 – volume: 107 start-page: 1047 year: 2010 ident: 10.1016/j.bbagen.2020.129648_bb0100 article-title: Microparticles protagonists of a novel communication network for intercellular information exchange publication-title: Circ. Res. doi: 10.1161/CIRCRESAHA.110.226456 – volume: 2 year: 2013 ident: 10.1016/j.bbagen.2020.129648_bb0150 article-title: As we wait: coping with an imperfect nomenclature for extracellular vesicles publication-title: J. Extracell. Vesicl. doi: 10.3402/jev.v2i0.20389 – year: 2015 ident: 10.1016/j.bbagen.2020.129648_bb0165 article-title: Transmission electron microscopy of biological samples publication-title: Transm. Electron Microsc. Theory Appl. – volume: 6 year: 2019 ident: 10.1016/j.bbagen.2020.129648_bb0200 article-title: Extracellular vesicle quantification and characterization: common methods and emerging approaches publication-title: Bioengineering doi: 10.3390/bioengineering6010007 – volume: 9 year: 2020 ident: 10.1016/j.bbagen.2020.129648_bb0090 article-title: Apoptotic bodies: Particular extracellular vesicles involved in intercellular communication publication-title: Biology (Basel) – volume: 192 start-page: 262 year: 2014 ident: 10.1016/j.bbagen.2020.129648_bb0050 article-title: Paclitaxel is incorporated by mesenchymal stromal cells and released in exosomes that inhibit in vitro tumor growth: a new approach for drug delivery publication-title: J. Control. Release doi: 10.1016/j.jconrel.2014.07.042 – volume: 1660 start-page: 221 year: 2017 ident: 10.1016/j.bbagen.2020.129648_bb0220 article-title: Detection and characterization of extracellular vesicles by transmission and cryo-transmission electron microscopy publication-title: Methods Mol. Biol. doi: 10.1007/978-1-4939-7253-1_18 – volume: 27 start-page: 5638 year: 2015 ident: 10.1016/j.bbagen.2020.129648_bb0355 article-title: Electron tomography: a three-dimensional analytic tool for hard and soft materials research publication-title: Adv Mater. doi: 10.1002/adma.201501015 – volume: 202 start-page: 407 year: 2013 ident: 10.1016/j.bbagen.2020.129648_bb0365 article-title: Cryo-electron tomography: the challenge of doing structural biology in situ publication-title: J. Cell Biol. doi: 10.1083/jcb.201304193 – volume: 31 start-page: 2892 year: 2001 ident: 10.1016/j.bbagen.2020.129648_bb0120 article-title: ‘Tolerosomes’ are produced by intestinal epithelial cells publication-title: Eur. J. Immunol. doi: 10.1002/1521-4141(2001010)31:10<2892::AID-IMMU2892>3.0.CO;2-I – volume: 1545 start-page: 139 year: 2014 ident: 10.1016/j.bbagen.2020.129648_bb0320 – volume: 30 start-page: 1 issue: 1 year: 2006 ident: 10.1016/j.bbagen.2020.129648_bb0205 article-title: Isolation and characterization of exosomes from cell culture supernatants publication-title: Curr. Protoc. Cell Biol. doi: 10.1002/0471143030.cb0322s30 – volume: 10 start-page: 111 year: 2017 ident: 10.1016/j.bbagen.2020.129648_bb0400 article-title: Purification protocols for extracellular vesicles publication-title: Extracell. Vesicles – volume: 56 start-page: 254 year: 2017 ident: 10.1016/j.bbagen.2020.129648_bb0425 article-title: Extracellular vesicle characteristics in stored red blood cell concentrates are influenced by the method of detection publication-title: Transfus. Apher. Sci. doi: 10.1016/j.transci.2017.03.007 – volume: 2016 year: 2016 ident: 10.1016/j.bbagen.2020.129648_bb0300 article-title: MiRNAs in urine extracellular vesicles as predictors of early-stage diabetic nephropathy publication-title: J. Diabetes Res. doi: 10.1155/2016/7932765 – volume: 130 start-page: 877 year: 2008 ident: 10.1016/j.bbagen.2020.129648_bb0195 article-title: Electron microscopy of high pressure frozen samples: bridging the gap between cellular ultrastructure and atomic resolution publication-title: Histochem. Cell Biol. doi: 10.1007/s00418-008-0500-1 – volume: 376 start-page: 149 year: 2019 ident: 10.1016/j.bbagen.2020.129648_bb0265 article-title: Correlative light and electron microscopy is a powerful tool to study interactions of extracellular vesicles with recipient cells publication-title: Exp. Cell Res. doi: 10.1016/j.yexcr.2019.02.004 – volume: 90 start-page: 1549 year: 2010 ident: 10.1016/j.bbagen.2020.129648_bb0015 article-title: Role of extracellular membrane vesicles in the pathogenesis of various diseases, including cancer, renal diseases, atherosclerosis, and arthritis publication-title: Lab. Investig. doi: 10.1038/labinvest.2010.152 – volume: 3 start-page: 1 year: 2014 ident: 10.1016/j.bbagen.2020.129648_bb0155 article-title: Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles publication-title: J. Extracell. Vesicles. doi: 10.3402/jev.v3.26913 – volume: 103 start-page: 192 year: 1970 ident: 10.1016/j.bbagen.2020.129648_bb0135 article-title: Fine structure and histochemistry of ‘calcifying globules’ in epiphyseal cartilage publication-title: Zeitschrift Für Zellforsch. Und Mikroskopische Anat. doi: 10.1007/BF00337312 – volume: 8 year: 2019 ident: 10.1016/j.bbagen.2020.129648_bb0215 article-title: Quality of extracellular vesicle images by transmission electron microscopy is operator and protocol dependent publication-title: J. Extracell. Vesicles doi: 10.1080/20013078.2018.1555419 – volume: 26 start-page: 4160 year: 2012 ident: 10.1016/j.bbagen.2020.129648_bb0240 article-title: Prion-infected cells regulate the release of exosomes with distinct ultrastructural features publication-title: FASEB J. doi: 10.1096/fj.11-202077 – volume: 120 start-page: 1632 year: 2017 ident: 10.1016/j.bbagen.2020.129648_bb0160 article-title: Methodological guidelines to study extracellular vesicles publication-title: Circ. Res. doi: 10.1161/CIRCRESAHA.117.309417 – year: 1967 ident: 10.1016/j.bbagen.2020.129648_bb0010 – volume: 8 year: 2013 ident: 10.1016/j.bbagen.2020.129648_bb0235 article-title: Cryogenic transmission electron microscopy nanostructural study of shed microparticles publication-title: PLoS One doi: 10.1371/journal.pone.0083680 – volume: 4 start-page: 28680 year: 2015 ident: 10.1016/j.bbagen.2020.129648_bb0375 article-title: Diversity of extracellular vesicles in human ejaculates revealed by cryo-electron microscopy publication-title: J. Extracell. Vesicles doi: 10.3402/jev.v4.28680 – volume: 90 start-page: 481 year: 2017 ident: 10.1016/j.bbagen.2020.129648_bb0005 article-title: Extracellular vesicles: evolutionarily conserved mediators of intercellular communication publication-title: Yale J. Biol. Med. – volume: 39 start-page: 184 year: 2003 ident: 10.1016/j.bbagen.2020.129648_bb0410 article-title: Elevated levels of circulating platelet microparticles, VEGF, IL-6 and RANTES in patients with gastric cancer : possible role of a metastasis predictor publication-title: Eur. J. Cancer doi: 10.1016/S0959-8049(02)00596-8 – year: 2015 ident: 10.1016/j.bbagen.2020.129648_bb0170 – volume: 2019 year: 2019 ident: 10.1016/j.bbagen.2020.129648_bb0380 article-title: Equine adipose-derived mesenchymal stromal cells release extracellular vesicles enclosing different subsets of small RNAs publication-title: Stem Cells Int. doi: 10.1155/2019/4957806 – volume: 51 year: 2019 ident: 10.1016/j.bbagen.2020.129648_bb0030 article-title: Extracellular vesicle-based therapeutics: natural versus engineered targeting and trafficking publication-title: Exp. Mol. Med. doi: 10.1038/s12276-019-0223-5 – volume: 17 start-page: 879 year: 2005 ident: 10.1016/j.bbagen.2020.129648_bb0060 article-title: Exosomal-like vesicles are present in human blood plasma publication-title: Int. Immunol. doi: 10.1093/intimm/dxh267 – year: 2015 ident: 10.1016/j.bbagen.2020.129648_bb0175 – volume: 10 start-page: 332 year: 2019 ident: 10.1016/j.bbagen.2020.129648_bb0255 article-title: Extracellular vesicles derived from human Wharton’s jelly mesenchymal stem cells protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-β oligomers publication-title: Stem Cell Res Ther doi: 10.1186/s13287-019-1432-5 – volume: 101 start-page: 13368 year: 2004 ident: 10.1016/j.bbagen.2020.129648_bb0065 article-title: Identification and proteomic profiling of exosomes in human urine publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.0403453101 – volume: 118 start-page: 2849 year: 2005 ident: 10.1016/j.bbagen.2020.129648_bb0115 article-title: Release of extracellular membrane particles carrying the stem cell marker prominin-1 (CD133) from neural progenitors and other epithelial cells publication-title: J. Cell Sci. doi: 10.1242/jcs.02439 – volume: 4 year: 2017 ident: 10.1016/j.bbagen.2020.129648_bb0285 article-title: Transmission electron microscopy as a tool for the characterization of soft materials: application and interpretation publication-title: Adv. Sci. doi: 10.1002/advs.201600476 – volume: 41 start-page: 59 year: 1969 ident: 10.1016/j.bbagen.2020.129648_bb0130 article-title: Vesicles associated with calcification in the matrix of epiphyseal cartilage publication-title: J. Cell Biol. doi: 10.1083/jcb.41.1.59 – volume: 28 start-page: 263 year: 2017 ident: 10.1016/j.bbagen.2020.129648_bb0250 article-title: Extracellular vesicles from activated platelets: a semiquantitative cryo-electron microscopy and immuno-gold labeling study publication-title: Platelets doi: 10.1080/09537104.2016.1268255 – volume: 2018 start-page: 5 year: 2018 ident: 10.1016/j.bbagen.2020.129648_bb0290 article-title: Sample preparation and imaging of exosomes by transmission electron microscopy publication-title: J. Vis. Exp. – volume: 2019 year: 2019 ident: 10.1016/j.bbagen.2020.129648_bb0230 article-title: Equine adipose-derived mesenchymal stromal cells release extracellular vesicles enclosing different subsets of small RNAs publication-title: Stem Cells Int. doi: 10.1155/2019/4957806 – volume: 179 start-page: 1969 year: 2007 ident: 10.1016/j.bbagen.2020.129648_bb0080 article-title: Exosomes with immune modulatory features are present in human breast milk publication-title: J. Immunol. doi: 10.4049/jimmunol.179.3.1969 – volume: 7 year: 2018 ident: 10.1016/j.bbagen.2020.129648_bb0025 article-title: Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines publication-title: J. Extracell. Vesicl. doi: 10.1080/20013078.2018.1535750 – volume: 40 start-page: 89 year: 2015 ident: 10.1016/j.bbagen.2020.129648_bb0345 article-title: Extracellular vesicles - their role in the packaging and spread of misfolded proteins associated with neurodegenerative diseases publication-title: Semin. Cell Dev. Biol. doi: 10.1016/j.semcdb.2015.02.007 – volume: 75 start-page: 2873 year: 2018 ident: 10.1016/j.bbagen.2020.129648_bb0395 article-title: Detailed analysis of the plasma extracellular vesicle proteome after separation from lipoproteins publication-title: Cell. Mol. Life Sci. doi: 10.1007/s00018-018-2773-4 – volume: 200 start-page: 373 year: 2013 ident: 10.1016/j.bbagen.2020.129648_bb0055 article-title: Extracellular vesicles: Exosomes, microvesicles, and friends publication-title: J. Cell Biol. doi: 10.1083/jcb.201211138 – volume: 87 start-page: 146 year: 2011 ident: 10.1016/j.bbagen.2020.129648_bb0180 article-title: Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy publication-title: Colloid. Surf. B Biointerf. doi: 10.1016/j.colsurfb.2011.05.013 – volume: 1 start-page: 4 year: 2016 ident: 10.1016/j.bbagen.2020.129648_bb0125 article-title: Oncosomes - large and small what are they, where they came from? publication-title: J. Extracell. Vesicl. – volume: 22 start-page: 2001 year: 2018 ident: 10.1016/j.bbagen.2020.129648_bb0245 article-title: Physical exosome:exosome interactions publication-title: J. Cell. Mol. Med. doi: 10.1111/jcmm.13479 |
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| Title | Imaging extracelluar vesicles by transmission electron microscopy: Coping with technical hurdles and morphological interpretation |
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