De novo DNA methyltransferase DNMT3A: Regulation of oligomeric state and mechanism of action in response to pH changes

• Published in: Biochimica et biophysica acta Vol. 1850; no. 6; pp. 1131 - 1139
• Main Authors: Holz-Schietinger, Celeste, Reich, Norbert O.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2015
• Subjects: Cancer; DNA; DNA (Cytosine-5-)-Methyltransferases - chemistry; DNA (Cytosine-5-)-Methyltransferases - metabolism; DNA Methylation; DNMT3A; Enzyme Activation; fluorescence; gel chromatography; Humans; Hydrogen-Ion Concentration; Kinetics; mechanism of action; methyltransferases; Models, Molecular; neoplasm cells; neoplasms; Oligomers; Processivity; Protein Conformation; Protein Multimerization; Structure-Activity Relationship; Oligomers; DNA methylation; pH; Processivity; DNMT3A; Cancer

The oligomeric state of the human DNMT3A is functionally important and cancer cells are known to undergo changes in pH (intracellular). Light scattering, gel filtration, and fluorescence anisotropy. Also, methylation and processivity assays. Physiologically relevant changes in pH result in changes i...