The influence of orexins on ethanol-induced behavioral sensitization in male mice
•Ethanol-induced behavioral sensitization activated orexin neurons in the LH.•Blockade of orexin 1 receptors prevented expression of behavioral sensitization.•Orexin system participates in ethanol-induced behavioral sensitization in mice. Recent evidence indicates the involvement of orexin in reward...
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Published in | Neuroscience letters Vol. 551; pp. 84 - 88 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Ireland
Elsevier Ireland Ltd
13.09.2013
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Subjects | |
Online Access | Get full text |
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Summary: | •Ethanol-induced behavioral sensitization activated orexin neurons in the LH.•Blockade of orexin 1 receptors prevented expression of behavioral sensitization.•Orexin system participates in ethanol-induced behavioral sensitization in mice.
Recent evidence indicates the involvement of orexin in reward circuitry and drug addiction. In the present study we evaluated the role of orexin in ethanol-induced behavioral sensitization. In the first experiment, Swiss male mice received seven administrations of saline or ethanol (2.2g/kg, i.p., chronic), every other day. On the last day of treatment, half of saline-treated mice received a saline injection (saline) whereas the other half received 2.2g/kg of ethanol (i.p., acute). Behavioral sensitization was assessed by locomotor activity tests and after the last one, immunoreactivity for orexin and Fos (ORX+Fos-ir) was assessed in the lateral hypothalamic area. Chronic ethanol treatment produced behavioral sensitization and a trend for greater ORX+Fos-ir. In the second experiment, mice were treated as in Experiment 1 and type 1 orexin receptor antagonist, SB334867 (20mg/kg), was administered before the ethanol challenge successfully blocking the expression of sensitization in mice chronically treated with EtOH. These results indicate that orexin plays a role in ethanol-induced behavioral sensitization. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0304-3940 1872-7972 |
DOI: | 10.1016/j.neulet.2013.07.010 |