Yacon ( Polymnia sanchifolia) extract as a substrate to produce inulinase by Kluyveromyces marxianus var. bulgaricus

• Published in: Journal of food engineering Vol. 66; no. 3; pp. 301 - 305
• Main Authors: Cazetta, M.L., Martins, P.M.M., Monti, R., Contiero, J.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.02.2005; Elsevier
• Subjects: Biological and medical sciences; biological production; biosynthesis; enzyme activity; fermentation; Food engineering; Food industries; Fructooligosaccharides; Fundamental and applied biological sciences. Psychology; General aspects; Inulin; Inulinase; Kluyveromyces marxianus; Kluyveromyces marxianus var. bulgaricus; Kluyveromyces marxianus var. lactis; medicinal plants; plant extracts; Polymnia; Polymnia sanchifolia; Yacon; Yacon; Kluyveromyces marxianus var. bulgaricus; Polymnia sanchifolia; Inulinase; Inulin; Fructooligosaccharides; Yeast; Enzyme; Oligosaccharide; Extract; Fungi; Yacon: Inulinase; Glycosidases; Hydrolases; Kluyveromyces marxianus; Ascomycetes; O-Glycosidases; Polysaccharide; Thallophyta
• ISSN: 0260-8774; 1873-5770
• DOI: 10.1016/j.jfoodeng.2004.03.022

The inulinase production by yeast K. marxianus var. bulgaricus growing in yacon extract was investigated. The microorganism showed good development in yacon, higher enzymatic activities were achieved at 30% and 40% (v/v) of extract. The cultivation temperature (20, 25, 30, 35, 40 °C) neither influenced the growth or the enzymatic activity. The optimum cultivation pH was 3.5. The highest activity was observed at 60 °C and pH 4.0. At temperature of 55 °C and 60 °C occurred sharp decrease in the enzyme activity.