Structural and biochemical insights into the degradation mechanism of chitosan by chitosanase OU01

• Published in: Biochimica et biophysica acta Vol. 1850; no. 9; pp. 1953 - 1961
• Main Authors: Lyu, Qianqian, Shi, Yanhong, Wang, Song, Yang, Yan, Han, Baoqin, Liu, Wanshun, Jones, David N.M., Liu, Weizhi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2015
• Subjects: Biocatalysis; catalytic activity; Chitosan; Chitosan - metabolism; chitosanase; crystal structure; Degradation; Glycoside Hydrolases - chemistry; Glycoside Hydrolases - metabolism; Glycoside hydrolysis; hydrolysis; Models, Molecular; Mutagenesis; mutants; Protein Structure, Tertiary; X-ray crystallography; Degradation; X-ray crystallography; Glycoside hydrolysis; Chitosan; Mutagenesis

A detailed knowledge about the degradation mechanism of chitosanase hydrolysis is critical for the design of novel enzymes to produce well-defined chito-oligosaccharide products. Through the combination of structural and biochemical analysis, we present new findings that provide novel insights into...