Quercetin modulates OTA-induced oxidative stress and redox signalling in HepG2 cells — up regulation of Nrf2 expression and down regulation of NF-κB and COX-2

• Published in: Biochimica et biophysica acta Vol. 1840; no. 1; pp. 681 - 692
• Main Authors: Ramyaa, Periasamy, krishnaswamy, Rajashree, Padma, Viswanadha Vijaya
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2014
• Subjects: anti-inflammatory activity; antidotes; Antioxidants - pharmacology; Apoptosis - drug effects; Blotting, Western; calcium; Calcium - metabolism; Calcium Channel Blockers - pharmacology; Cell Proliferation - drug effects; Cyclooxygenase 2 - metabolism; Cytokinesis - drug effects; DNA damage; Fluorescent Antibody Technique; Gene Expression Regulation, Neoplastic - drug effects; Glutathione - metabolism; Hep G2 Cells; human cell lines; Humans; Inflammation; kidney cells; Lipid Peroxidation - drug effects; liver; Micronucleus Tests; NF-E2-Related Factor 2 - metabolism; NF-kappa B - metabolism; Nitrites - metabolism; Ochratoxin; ochratoxin A; Ochratoxins - pharmacology; Oxidation-Reduction; Oxidative stress; Oxidative Stress - drug effects; Protein Carbonylation - drug effects; Quercetin; Quercetin - pharmacology; Reactive Oxygen Species - metabolism; toxicity; transcription factor NF-kappa B; NO; Ochratoxin; Oxidative stress; COX-2; DTT; DNA damage; LPO; MTT; LPS; DMEM; LDH; NF-κB; CCCP; OPT; ANOVA; FBS; iNOS; GSH; CBMN; MN; [Ca2+]i; DAB; AO/EB; DCF; Nrf-2; DAPI; GST; SOD; Inflammation; EDTA; GPx; PCC; DiOC6; ARE; PMSF; CAT; ROS; Quercetin; DCF-DA; IC20; OTA; Nuclear factor-kappa B; 3,30-di-hexyloxacarbocyanine iodide; Intracellular calcium level; Cytokinesis block micronucleus assay; Lipid peroxidation; Carbonyl cyanide m-chlorophenylhydrazone; Protein carbonyl content; Lipopolysaccharide; 2,7-dichlorofluorescein; Dithiothreitol; Glutathione peroxidase; Fetal bovine serum; Phenyl methyl sulfonyl fluoride; Antioxidant responsive element; Dulbecco's modified Eagle's medium; 4,4-diamidino-2-phenylindole; Nitric oxide; Nuclear factor E2 p45-related factor 2; Analysis of variance; Reactive oxygen species; 2,7-dichlorodihydrofluorescein diacetate; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; Superoxide dismutase; Catalase; Inducible nitric oxide synthase; Ethylenediamine tetraacetic acid; Micronucleus; Acridine orange/ethidium bromide; Ortho-phthalaldehyde; Glutathione; Inhibitory concentration 20; Lactate dehydrogenase; [Ca(2+)](i); Glutathione S transferase; Cyclooxygenase-2; Diaminobenzidine

Ochratoxin A (OTA), a mycotoxin, causes extensive cell damage, affecting liver and kidney cells. OTA toxicity is fairly well characterized where oxidative stress is believed to play a role, however, the sequence of molecular events after OTA-exposure, have not been characterized in literature. Furth...