Fatty acid synthase inhibitor orlistat induces apoptosis in T cell lymphoma: Role of cell survival regulatory molecules

• Published in: Biochimica et biophysica acta Vol. 1820; no. 11; pp. 1764 - 1773
• Main Authors: Kant, Shiva, Kumar, Ajay, Singh, Sukh Mahendra
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2012
• Subjects: animal models; Animals; Antineoplastic Agents - pharmacology; Apoptosis; Apoptosis - drug effects; caspase-3; Cell survival; Cell Survival - drug effects; cell viability; cytokines; Cytokines - biosynthesis; DNA fragmentation; Dose-Response Relationship, Drug; enzyme-linked immunosorbent assay; Fatty acid synthase; Fatty Acid Synthases - antagonists & inhibitors; fatty acids; fluorescence microscopy; gene expression; HSP70 Heat-Shock Proteins - physiology; interferon-gamma; Interferon-gamma - physiology; Lactones - pharmacology; Lymphoma, T-Cell - drug therapy; Lymphoma, T-Cell - pathology; Male; Mice; Mice, Inbred BALB C; neoplasm cells; Orlistat; proteins; reactive oxygen species; Reactive Oxygen Species - metabolism; reverse transcriptase polymerase chain reaction; T cell lymphoma; T-lymphocytes; Western blotting; NADPH; Cell survival; FASN; T cell lymphoma; ROS; DL; Orlistat; DCFDA; Fatty acid synthase; PUMA; CPT-1; Apoptosis

De novo fatty acid synthesis catalyzed by fatty acid synthase (FASN) is crucial for tumor cell survival. Thus therapeutic targeting of FASN is considered as a novel antineoplastic strategy. However, little is understood in this respect regarding malignancies of hematological origin. The present inve...