In vivo visualization of murine melanoma cells B16-derived exosomes through magnetic resonance imaging

• Published in: Biochimica et biophysica acta. General subjects Vol. 1866; no. 2; p. 130062
• Main Authors: Liu, Tianqi, Li, Zhenlin, Li, Xiaodong, Zhao, Ruiting, Wei, Xinhua, Wang, Zixin, Xin, Sherman Xuegang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2022
• Subjects: Animals; Antigens, Surface; Apoferritins - chemistry; Apoferritins - metabolism; cell communication; Cell Line, Tumor; exosomes; Exosomes - metabolism; Exosomes self-labeling; ferritin; lactadherin; Magnetic resonance imaging (MRI); Magnetic Resonance Imaging - methods; magnetism; melanoma; Melanoma, Experimental - diagnostic imaging; Melanoma, Experimental - metabolism; Melanoma, Experimental - pathology; metastasis; Mice; Mice, Inbred C57BL; Milk Proteins; MRI reporter protein; neoplasm progression; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; tissues; transmission electron microscopy; Western blotting; MRI; DMSO; PFA; USPIO; MTT; UA; MTX; DMEM; SD; ANOVA; FTH1; FBS; PBS; BCA; EVs; qRT-PCR; 3 T; Magnetic resonance imaging (MRI); FOV; PVDF; ECL; SDS-PAGE; TE; NTA; FA; ETL; Exosomes self-labeling; MRI reporter protein; TEM; TR

Numerous studies demonstrated that exosomes play a powerful role in mediating intercellular communication to induce a pro-tumoral environment to promote tumor progression, including pre-metastatic niche formation and metastasis. Noninvasive imaging could determine the in vivo kinetics of exosomes in...