Inosine 5′-diphosphate, a molecular decoy rescues Nucleoside diphosphate kinase from c-MYC G-Quadruplex unfolding

• Published in: Biochimica et biophysica acta. General subjects Vol. 1864; no. 9; p. 129649
• Main Authors: Sengupta, Pallabi, Chatterjee, Subhrangsu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2020
• Subjects: amino acids; apoptosis; c-MYC; Cell cycle; Cell Line, Tumor; chromatin immunoprecipitation; epitopes; G-Quadruplex; G-Quadruplexes; G2 Phase Cell Cycle Checkpoints; gene expression regulation; geometry; guanosine diphosphate; Humans; hydrogen bonding; inosine; Inosine diphosphate; Inosine Diphosphate - metabolism; M Phase Cell Cycle Checkpoints; Models, Molecular; moieties; NM23-H2; nucleoside-diphosphate kinase; Nucleoside-Diphosphate Kinase - chemistry; Nucleoside-Diphosphate Kinase - metabolism; Promoter Regions, Genetic - genetics; Protein Conformation; Proto-Oncogene Proteins c-myc - genetics; pyrophosphates; therapeutics; Transcription; Transcription, Genetic; Inosine diphosphate; Transcription; NM23-H2; G-Quadruplex; c-MYC; Cell cycle

The transcription-inhibitory G-Quadruplex(Pu27-GQ) at c-MYC promoter is challenging to target due to structural heterogeneity. Nucleoside diphosphate kinase (NM23-H2) specifically binds and unfolds Pu27-GQ to increase c-MYC transcription. Here, we used Inosine 5′-diphosphate (IDP) to disrupt NM23-H2...