A rapid and efficient strategy for combinatorial repression of multiple genes in Escherichia coli

• Published in: Microbial cell factories Vol. 24; no. 1; pp. 74 - 12
• Main Authors: Zheng, Yi, Mo, Yuxia, Yuan, Yingbo, Su, Tianyuan, Qi, Qingsheng
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 28.03.2025; BMC
• Subjects: Analysis; Biosynthesis; Combinatorial repression; CRISPR-Cas Systems; CRISPRi; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Gene expression; Gene Expression Regulation, Bacterial; Genetic aspects; Identification and classification; Inducible promoters; Metabolic Engineering - methods; Metabolic flow; Multiple genes; Plasmids - genetics; Plasmids - metabolism; Promoter Regions, Genetic; RNA, Guide, CRISPR-Cas Systems - genetics; Japan; Combinatorial repression; Multiple genes; Inducible promoters; CRISPRi; Metabolic flow

The regulation of multiple gene expression is pivotal for metabolic engineering. Although CRISPR interference (CRISPRi) has been extensively utilized for multi-gene regulation, the construction of numerous single-guide RNA (sgRNA) expression plasmids for combinatorial regulation remains a significan...