Occurrence and seasonal variation of equine estrogens, equilin and equilenin, in the river water of Japan: Implication with endocrine-disrupting potentials to Japanese medaka (Oryzias latipes)

• Published in: Environmental pollution (1987) Vol. 239; pp. 281 - 288
• Main Authors: Ishibashi, Hiroshi, Uchida, Masaya, Yoshimoto, Keisuke, Imamura, Yuta, Yamamoto, Ryoko, Ikenaka, Yoshinori, Kawai, Masahito, Ichikawa, Nobuhiro, Takao, Yuji, Tominaga, Nobuaki, Ishibashi, Yasuhiro, Arizono, Koji
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2018
• Subjects: cytochrome P-450; eggs; endocrine-disrupting chemicals; equilin; Equine estrogen; estrogen receptors; Estrogen-responsive gene; fecundity; gene expression; gene expression regulation; genes; hatching; horses; Japan; LC–MS/MS; liquid chromatography; liver; males; messenger RNA; Oryzias latipes; pregnanes; quantitative polymerase chain reaction; Reproduction; reverse transcriptase polymerase chain reaction; river water; seasonal variation; tandem mass spectrometry; transcription (genetics); Transgeneration; vitellogenin; Japan; Estrogen-responsive gene; Equine estrogen; Reproduction; Transgeneration; Oryzias latipes; LC–MS/MS
• ISSN: 0269-7491; 1873-6424; 1873-6424
• DOI: 10.1016/j.envpol.2018.04.029

In this study, we determined the concentration of equine estrogens, such as equilin (Eq) and equilenin (Eqn), in the river water collected from nine research stations in Hokkaido, Japan. The LC–MS/MS analysis revealed that Eq concentrations were 2.7 ± 6.7, 0.22 ± 0.12, and 1.2 ± 0.64 ng/L in Sep 201...