The repressor Rgt1 and the cAMP-dependent protein kinases control the expression of the SUC2 gene in Saccharomyces cerevisiae

• Published in: Biochimica et biophysica acta Vol. 1850; no. 7; pp. 1362 - 1367
• Main Authors: Gancedo, Juana M., Flores, Carmen-Lisset, Gancedo, Carlos
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2015
• Subjects: Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; beta-fructofuranosidase; beta-Fructofuranosidase - genetics; beta-Fructofuranosidase - metabolism; Binding Sites - genetics; cAMP-dependent protein kinase; carbon; Cyclic AMP-Dependent Protein Kinase Catalytic Subunits - genetics; Cyclic AMP-Dependent Protein Kinase Catalytic Subunits - metabolism; Cyclic AMP-Dependent Protein Kinases - genetics; Cyclic AMP-Dependent Protein Kinases - metabolism; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; ethanol; Ethanol - pharmacology; Galactose - pharmacology; Gene Expression Regulation, Enzymologic - drug effects; Gene Expression Regulation, Fungal - drug effects; genes; Glucose; Glucose - pharmacology; glucose transporters; glycerol; Invertase; Isoenzymes - genetics; Isoenzymes - metabolism; lactic acid; Mutation; PKA; Promoter Regions, Genetic - genetics; regulatory sequences; Rgt1; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; SUC2; Transcription Factors - genetics; Transcription Factors - metabolism; Yeast; yeasts; Yeast; Glucose; Rgt1; SUC2; Invertase; PKA

A low level of glucose is required for maximal transcription of the SUC2 gene in Saccharomyces cerevisiae. Although the repressor Rgt1 binds the SUC2 promoter in gel-shift assays, it has been reported that Rgt1 has only minimal effects on SUC2 expression. Rgt1 acts together with Mth1 to repress the...