An efficient system for incorporation of unnatural amino acids in response to the four-base codon AGGA in Escherichia coli

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 11; pp. 3016 - 3023
• Main Authors: Lee, Byeong Sung, Kim, Suyeon, Ko, Byoung Joon, Yoo, Tae Hyeon
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2017
• Subjects: amino acid derivatives; amino acids; Amino Acids - chemical synthesis; Amino Acids - metabolism; Amino Acyl-tRNA Synthetases - metabolism; aminoacyl tRNA ligases; ArgW gene; Cloning, Molecular - methods; Codon - chemical synthesis; Engineered tRNA; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Four-base AGGA codon; Genetic Code; Methanococcus; Methanococcus - genetics; Mutagenesis, Site-Directed - methods; protein engineering; Protein Engineering - methods; protein synthesis; proteins; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; stop codon; synthetic biology; Synthetic Biology - methods; transfer RNA; Unnatural amino acid; Engineered tRNA; ArgW gene; Unnatural amino acid; Escherichia coli; Four-base AGGA codon

Adding new amino acids to the set of building blocks for protein synthesis expands the scope of protein engineering, and orthogonal pairs of tRNA and aminoacyl-tRNA synthetase have been developed for incorporating unnatural amino acids (UAAs) into proteins. While diverse systems have been developed...