N-glycosylation-mutated HCV envelope glycoprotein complex enhances antigen-presenting activity and cellular and neutralizing antibody responses - Ren, Yushan; Min, Yuan-Qin; Liu, Min; Chi, Lianli; Zhao, Ping; Zhang, Xiao-Lian (Biochimica et biophysica acta, 2016)
Keywords: Amino Acid Substitution; Animals; Antibodies, Neutralizing - immunology; antibody formation; Antigen Presentation; antigens; CD8-Positive T-Lymphocytes; Cell Line; cell-mediated immunity; Cellular immune response; CpG Islands; dendritic cells; Dendritic Cells - immunology; DNA; DNA vaccine; Female; genotype; glycoproteins; glycosylation; HEK293 Cells; HeLa Cells; Hepacivirus - genetics; Hepacivirus - immunology; Hepatitis C - genetics; Hepatitis C - immunology; Hepatitis C Antibodies - immunology; Hepatitis C virus; hepatocytes; Humans; interleukin-12; medicine; Mice; Mice, Inbred BALB C; mutants; mutation; Mutation, Missense; N-glycosylation; Neutralization; neutralizing antibodies; polysaccharides; recombinant vaccines; Th1 Cells - immunology; vaccination; Vaccines, DNA - genetics; Vaccines, DNA - immunology; Vaccines, DNA - pharmacology; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Viral Hepatitis Vaccines - genetics; Viral Hepatitis Vaccines - immunology; DNA vaccine; half-maximal effective concentration; oligodeoxynucleotides; reverse transcription-quantitative real time PCR; cell culture-derived HCV; interferon; enzyme-linked immunospot assay; N-glycosylation; multiplicity of infection; Antigen presenting cell; spot forming cells; HCV pseudo particles; interleukin; Cytotoxic T lymphocyte; Cellular immune response; Hepatitis C Virus; Neutralization; Hepatitis C virus