High-speed near-field fluorescence microscopy combined with high-speed atomic force microscopy for biological studies

• Published in: Biochimica et biophysica acta. General subjects Vol. 1864; no. 2; p. 129325
• Main Authors: Umakoshi, Takayuki, Fukuda, Shingo, Iino, Ryota, Uchihashi, Takayuki, Ando, Toshio
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2020
• Subjects: accuracy; atomic force microscopy; DNA; fluorescence microscopy; High-speed atomic force microscopy; image analysis; light microscopy; Plasmonics; proteins; Scanning near-field fluorescence microscopy; Single molecule analysis; Super-resolution optical microscopy; Scanning near-field fluorescence microscopy; HS-AFM; Single molecule analysis; TIRFM; HS-SNOM; High-speed atomic force microscopy; Super-resolution optical microscopy; Plasmonics; SNOM

High-speed atomic force microscopy (HS-AFM) has successfully visualized a variety of protein molecules during their functional activity. However, it cannot visualize small molecules interacting with proteins and even protein molecules when they are encapsulated. Thus, it has been desired to achieve...