The 60 nm gold nanoparticles improve qPCR amplification efficiency through specific palindromic sequences (GGATCC or ACCGGT) in primers

• Published in: Biochimica et biophysica acta. General subjects Vol. 1868; no. 4; p. 130560
• Main Authors: Zeng, Ruyu, Du, Zhiqun, Ma, Hongliang, Meng, Xiuqiong, Li, Erhua, Li, Jiangchao
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2024
• Subjects: Animals; computer software; DNA; genes; Gold; Gold nanoparticle; Metal Nanoparticles; Mice; mutation; nanogold; oligodeoxyribonucleotides; Palindrome sequence; Plasmids; Primes; qPCR; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction - methods; SSB; DMSO; Palindrome sequence; Primes; UV–VIS; CNTs; qPCR; 60 nm- Au; SEM; TMAC; TEM; qRT–PCR; Gold nanoparticle; PCR

Polymerase chain reaction (PCR) technology and quantitative real-time PCR (qPCR) technology are widely used in clinical diagnosis and research, but amplification efficiency and sensitivity are still key problems for researchers. An increasing number of reports show that gold nanoparticles (AuNPs) ca...