Functional characterization of PhoPR two component system and its implication in regulating phosphate homeostasis in Bacillus anthracis

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 1; pp. 2956 - 2970
• Main Authors: Aggarwal, Somya, Somani, Vikas Kumar, Gupta, Vatika, Kaur, Jaspreet, Singh, Damini, Grover, Abhinav, Bhatnagar, Rakesh
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2017
• Subjects: Amino Acid Sequence; Amino Acids - metabolism; Animals; anthrax; Bacillus anthracis; Bacillus anthracis - drug effects; Bacillus anthracis - genetics; Bacillus anthracis - metabolism; Bacillus anthracis - pathogenicity; Bacterial Proteins - chemistry; Bacterial Proteins - metabolism; Base Sequence; Computer Simulation; DNA, Bacterial - metabolism; drugs; Gene Expression Regulation, Bacterial - drug effects; genetic markers; Histidine kinase; homeostasis; Homeostasis - drug effects; Mice; Molecular Dynamics Simulation; Operon - genetics; pathogenesis; pathogens; PhoPR; Phosphate starvation; phosphates; Phosphates - metabolism; Phosphates - pharmacology; Phosphorylation - drug effects; prokaryotic cells; Promoter Regions, Genetic; Protein Binding - drug effects; protein phosphorylation; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction; Reproducibility of Results; Response regulator; reverse transcriptase polymerase chain reaction; Sequence Homology, Amino Acid; Signal Transduction - drug effects; starvation; Survival Analysis; Two component system; Up-Regulation - drug effects; virulence; Virulence - drug effects; Virulence - genetics; RR; Bacillus anthracis; PhoPR; HK; PS; TCS; HRP; Response regulator; AcP; AP; Histidine kinase; EMSA; Phosphate starvation; Two component system; MBP; MSA; ELISA

Recent report on importance of phosphate starvation (PS) in Bacillus anthracis (BA) pathogenesis warrants further investigation of the underlying regulatory mechanism. Potential role of PhoPR two component system (TCS) in phosphate homeostasis and virulence of several pathogens necessitates the stud...