Reaction dynamics and residue identification of haemoglobin modification by acrolein, a lipid-peroxidation by-product

• Published in: Biochimica et biophysica acta. General subjects Vol. 1865; no. 12; p. 130013
• Main Authors: Lassé, Moritz, Stampfli, Anja R., Orban, Thomas, Bothara, Roshit K., Gerrard, Juliet A., Fairbanks, Antony J., Pattinson, Neil R., Dobson, Renwick C.J.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2021
• Subjects: Acrolein; Acrolein - chemistry; Acrolein - metabolism; Biomarker; byproducts; crosslinking; Diabetes; Haemoglobin; hemoglobin; Hemoglobins - chemistry; Hemoglobins - metabolism; Humans; Lipid Peroxidation; lipid peroxides; Liquid chromatography-mass spectrometry (LC-MS/MS); lysine; Lysine - chemistry; Lysine - metabolism; mass spectrometry; Mass Spectrometry - methods; Oxidative Stress; risk assessment; Oxidative stress; Acrolein; Haemoglobin; Biomarker; Diabetes; Liquid chromatography-mass spectrometry (LC-MS/MS)

Abstract Lipid hydroperoxides decompose to reactive aldehydes, such as acrolein. Measurement of oxidative stress markers in the clinic could improve risk stratification for patients. To aid the development of diagnostic oxidative stress markers, we defined the acrolein modifications of haemoglobin u...