Bioanalysis of underivatized amino acids in non-invasive exhaled breath condensate samples using liquid chromatography coupled with tandem mass spectrometry

• Published in: Journal of Chromatography A Vol. 1542; pp. 72 - 81
• Main Authors: Konieczna, Lucyna, Pyszka, Magdalena, Okońska, Magdalena, Niedźwiecki, Maciej, Bączek, Tomasz
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 23.03.2018
• Subjects: acetonitrile; Amino acids; ammonium; arginine; asparagine; biomarkers; children; clinical trials; condensates; cooling systems; derivatization; drug therapy; Exhaled breath condensate (EBC); glutamine; High-performance liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS); histidine; humans; hydroxyproline; ionization; Leukemia; liquid chromatography; Lyophilization; methionine; patients; proline; Sample preparation; tandem mass spectrometry; threonine; tyrosine; Vacuum evaporation; valine; Sample preparation; Leukemia; Amino acids; High-performance liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS); Lyophilization; Vacuum evaporation; Exhaled breath condensate (EBC)
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2018.02.019

•Pre-concentration step is necessary for amino acid analysis in EBC samples.•Vacuum evaporation is the best pre-concentration method for EBC samples.•The proposed LC-ESI–MS/MS method is sensitive, accurate, precise, and eliminates matrix interference in the simultaneous separation and quantification...