The challenges of using fluorescent probes to detect and quantify specific reactive oxygen species in living cells

• Published in: Biochimica et biophysica acta Vol. 1840; no. 2; pp. 730 - 738
• Main Author: Winterbourn, Christine C.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2014
• Subjects: Animals; biophysics; Boronate probe; catalysts; Cell Physiological Phenomena; cellular microenvironment; chemical interactions; Dichlorofluorescein; fluorescence; Fluorescent Dyes; Fluorescent probe; Humans; Hydrogen peroxide; membrane proteins; monitoring; oxidants; oxygen; Reactive Oxygen Species - analysis; Reactive oxygen species detection; Superoxide; GFP; Dichlorofluorescein; Hydrogen peroxide; DCF; Boronate probe; Fluorescent probe; SOD; Superoxide; Reactive oxygen species detection; superoxide dismutase; green fluorescent protein

Small molecule fluorescent probes are vital tools for monitoring reactive oxygen species in cells. The types of probe available, the extent to which they are specific or quantitative and complications in interpreting results are discussed. Most commonly used probes (e.g. dihydrodichlorofluorescein,...